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Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia

Engineered synthetic biological devices have been designed to perform a variety of functions from sensing molecules and bioremediation to energy production and biomedicine. Notwithstanding, a major limitation of in vivo circuit implementation is the constraint associated to the use of standard metho...

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Detalles Bibliográficos
Autores principales: Macia, Javier, Manzoni, Romilde, Conde, Núria, Urrios, Arturo, de Nadal, Eulàlia, Solé, Ricard, Posas, Francesc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734778/
https://www.ncbi.nlm.nih.gov/pubmed/26829588
http://dx.doi.org/10.1371/journal.pcbi.1004685
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author Macia, Javier
Manzoni, Romilde
Conde, Núria
Urrios, Arturo
de Nadal, Eulàlia
Solé, Ricard
Posas, Francesc
author_facet Macia, Javier
Manzoni, Romilde
Conde, Núria
Urrios, Arturo
de Nadal, Eulàlia
Solé, Ricard
Posas, Francesc
author_sort Macia, Javier
collection PubMed
description Engineered synthetic biological devices have been designed to perform a variety of functions from sensing molecules and bioremediation to energy production and biomedicine. Notwithstanding, a major limitation of in vivo circuit implementation is the constraint associated to the use of standard methodologies for circuit design. Thus, future success of these devices depends on obtaining circuits with scalable complexity and reusable parts. Here we show how to build complex computational devices using multicellular consortia and space as key computational elements. This spatial modular design grants scalability since its general architecture is independent of the circuit’s complexity, minimizes wiring requirements and allows component reusability with minimal genetic engineering. The potential use of this approach is demonstrated by implementation of complex logical functions with up to six inputs, thus demonstrating the scalability and flexibility of this method. The potential implications of our results are outlined.
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spelling pubmed-47347782016-02-04 Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia Macia, Javier Manzoni, Romilde Conde, Núria Urrios, Arturo de Nadal, Eulàlia Solé, Ricard Posas, Francesc PLoS Comput Biol Research Article Engineered synthetic biological devices have been designed to perform a variety of functions from sensing molecules and bioremediation to energy production and biomedicine. Notwithstanding, a major limitation of in vivo circuit implementation is the constraint associated to the use of standard methodologies for circuit design. Thus, future success of these devices depends on obtaining circuits with scalable complexity and reusable parts. Here we show how to build complex computational devices using multicellular consortia and space as key computational elements. This spatial modular design grants scalability since its general architecture is independent of the circuit’s complexity, minimizes wiring requirements and allows component reusability with minimal genetic engineering. The potential use of this approach is demonstrated by implementation of complex logical functions with up to six inputs, thus demonstrating the scalability and flexibility of this method. The potential implications of our results are outlined. Public Library of Science 2016-02-01 /pmc/articles/PMC4734778/ /pubmed/26829588 http://dx.doi.org/10.1371/journal.pcbi.1004685 Text en © 2016 Macia et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Macia, Javier
Manzoni, Romilde
Conde, Núria
Urrios, Arturo
de Nadal, Eulàlia
Solé, Ricard
Posas, Francesc
Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia
title Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia
title_full Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia
title_fullStr Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia
title_full_unstemmed Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia
title_short Implementation of Complex Biological Logic Circuits Using Spatially Distributed Multicellular Consortia
title_sort implementation of complex biological logic circuits using spatially distributed multicellular consortia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734778/
https://www.ncbi.nlm.nih.gov/pubmed/26829588
http://dx.doi.org/10.1371/journal.pcbi.1004685
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