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The effects of high-fat feeding on physical function and skeletal muscle extracellular matrix

Skeletal muscle extracellular matrix (ECM) remodelling has been proposed as a feature of the pathogenic milieu associated with obesity and metabolic dysfunction. Whether muscle ECM is associated with impaired physical function in obese conditions is unknown. C57BL/6 mice were fed a high-fat diet (HF...

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Autores principales: Tam, C S, Power, J E, Markovic, T P, Yee, C, Morsch, M, McLennan, S V, Twigg, S M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735053/
https://www.ncbi.nlm.nih.gov/pubmed/26657013
http://dx.doi.org/10.1038/nutd.2015.39
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author Tam, C S
Power, J E
Markovic, T P
Yee, C
Morsch, M
McLennan, S V
Twigg, S M
author_facet Tam, C S
Power, J E
Markovic, T P
Yee, C
Morsch, M
McLennan, S V
Twigg, S M
author_sort Tam, C S
collection PubMed
description Skeletal muscle extracellular matrix (ECM) remodelling has been proposed as a feature of the pathogenic milieu associated with obesity and metabolic dysfunction. Whether muscle ECM is associated with impaired physical function in obese conditions is unknown. C57BL/6 mice were fed a high-fat diet (HFD) or chow for 5, 10 and 25 weeks. Non-invasive physiological tests (hang wire, hang mesh and grip strength) to assess neuromuscular function and motor co-ordination were performed. Genes related to ECM structure (COL1, COL3, COL6A2, SPARC), growth factors (TGFB1, TGFB2, CTGF, VEGF) and muscle function (DMD (Dp147), CPN3, DAG1) were measured in gastrocnemius muscle using real-time PCR and COL1, 3 and 6 protein were measured by western immunoblot. Compared with chow, HFD mice had two to six-fold lower muscle strength (hang wire test; raw data and multiplied by body weight) at all time-points (P<0.001) and two-fold lower hang mesh and grip strength at 10 weeks (P<0.05). At 5 weeks, COL1, COL3 and COL6 gene expression, but not protein levels were three to eight-fold lower in HFD compared with chow. In the HFD group at 5 weeks, greater COL3 and 6 gene expression were associated with poorer hang wire performance. For the first time, our results demonstrate links between muscle ECM structure and physical function in obesity.
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spelling pubmed-47350532016-02-05 The effects of high-fat feeding on physical function and skeletal muscle extracellular matrix Tam, C S Power, J E Markovic, T P Yee, C Morsch, M McLennan, S V Twigg, S M Nutr Diabetes Short Communication Skeletal muscle extracellular matrix (ECM) remodelling has been proposed as a feature of the pathogenic milieu associated with obesity and metabolic dysfunction. Whether muscle ECM is associated with impaired physical function in obese conditions is unknown. C57BL/6 mice were fed a high-fat diet (HFD) or chow for 5, 10 and 25 weeks. Non-invasive physiological tests (hang wire, hang mesh and grip strength) to assess neuromuscular function and motor co-ordination were performed. Genes related to ECM structure (COL1, COL3, COL6A2, SPARC), growth factors (TGFB1, TGFB2, CTGF, VEGF) and muscle function (DMD (Dp147), CPN3, DAG1) were measured in gastrocnemius muscle using real-time PCR and COL1, 3 and 6 protein were measured by western immunoblot. Compared with chow, HFD mice had two to six-fold lower muscle strength (hang wire test; raw data and multiplied by body weight) at all time-points (P<0.001) and two-fold lower hang mesh and grip strength at 10 weeks (P<0.05). At 5 weeks, COL1, COL3 and COL6 gene expression, but not protein levels were three to eight-fold lower in HFD compared with chow. In the HFD group at 5 weeks, greater COL3 and 6 gene expression were associated with poorer hang wire performance. For the first time, our results demonstrate links between muscle ECM structure and physical function in obesity. Nature Publishing Group 2015-12 2015-12-14 /pmc/articles/PMC4735053/ /pubmed/26657013 http://dx.doi.org/10.1038/nutd.2015.39 Text en Copyright © 2015 Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Short Communication
Tam, C S
Power, J E
Markovic, T P
Yee, C
Morsch, M
McLennan, S V
Twigg, S M
The effects of high-fat feeding on physical function and skeletal muscle extracellular matrix
title The effects of high-fat feeding on physical function and skeletal muscle extracellular matrix
title_full The effects of high-fat feeding on physical function and skeletal muscle extracellular matrix
title_fullStr The effects of high-fat feeding on physical function and skeletal muscle extracellular matrix
title_full_unstemmed The effects of high-fat feeding on physical function and skeletal muscle extracellular matrix
title_short The effects of high-fat feeding on physical function and skeletal muscle extracellular matrix
title_sort effects of high-fat feeding on physical function and skeletal muscle extracellular matrix
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735053/
https://www.ncbi.nlm.nih.gov/pubmed/26657013
http://dx.doi.org/10.1038/nutd.2015.39
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