Cargando…

Cell wall as a target for bacteria inactivation by pulsed electric fields

The integrity and morphology of bacteria is sustained by the cell wall, the target of the main microbial inactivation processes. One promising approach to inactivation is based on the use of pulsed electric fields (PEF). The current dogma is that irreversible cell membrane electro-permeabilisation c...

Descripción completa

Detalles Bibliográficos
Autores principales: Pillet, Flavien, Formosa-Dague, Cécile, Baaziz, Houda, Dague, Etienne, Rols, Marie-Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735277/
https://www.ncbi.nlm.nih.gov/pubmed/26830154
http://dx.doi.org/10.1038/srep19778
_version_ 1782413051766505472
author Pillet, Flavien
Formosa-Dague, Cécile
Baaziz, Houda
Dague, Etienne
Rols, Marie-Pierre
author_facet Pillet, Flavien
Formosa-Dague, Cécile
Baaziz, Houda
Dague, Etienne
Rols, Marie-Pierre
author_sort Pillet, Flavien
collection PubMed
description The integrity and morphology of bacteria is sustained by the cell wall, the target of the main microbial inactivation processes. One promising approach to inactivation is based on the use of pulsed electric fields (PEF). The current dogma is that irreversible cell membrane electro-permeabilisation causes the death of the bacteria. However, the actual effect on the cell-wall architecture has been poorly explored. Here we combine atomic force microscopy and electron microscopy to study the cell-wall organization of living Bacillus pumilus bacteria at the nanoscale. For vegetative bacteria, exposure to PEF led to structural disorganization correlated with morphological and mechanical alterations of the cell wall. For spores, PEF exposure led to the partial destruction of coat protein nanostructures, associated with internal alterations of cortex and core. Our findings reveal for the first time that the cell wall and coat architecture are directly involved in the electro-eradication of bacteria.
format Online
Article
Text
id pubmed-4735277
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-47352772016-02-05 Cell wall as a target for bacteria inactivation by pulsed electric fields Pillet, Flavien Formosa-Dague, Cécile Baaziz, Houda Dague, Etienne Rols, Marie-Pierre Sci Rep Article The integrity and morphology of bacteria is sustained by the cell wall, the target of the main microbial inactivation processes. One promising approach to inactivation is based on the use of pulsed electric fields (PEF). The current dogma is that irreversible cell membrane electro-permeabilisation causes the death of the bacteria. However, the actual effect on the cell-wall architecture has been poorly explored. Here we combine atomic force microscopy and electron microscopy to study the cell-wall organization of living Bacillus pumilus bacteria at the nanoscale. For vegetative bacteria, exposure to PEF led to structural disorganization correlated with morphological and mechanical alterations of the cell wall. For spores, PEF exposure led to the partial destruction of coat protein nanostructures, associated with internal alterations of cortex and core. Our findings reveal for the first time that the cell wall and coat architecture are directly involved in the electro-eradication of bacteria. Nature Publishing Group 2016-02-01 /pmc/articles/PMC4735277/ /pubmed/26830154 http://dx.doi.org/10.1038/srep19778 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Pillet, Flavien
Formosa-Dague, Cécile
Baaziz, Houda
Dague, Etienne
Rols, Marie-Pierre
Cell wall as a target for bacteria inactivation by pulsed electric fields
title Cell wall as a target for bacteria inactivation by pulsed electric fields
title_full Cell wall as a target for bacteria inactivation by pulsed electric fields
title_fullStr Cell wall as a target for bacteria inactivation by pulsed electric fields
title_full_unstemmed Cell wall as a target for bacteria inactivation by pulsed electric fields
title_short Cell wall as a target for bacteria inactivation by pulsed electric fields
title_sort cell wall as a target for bacteria inactivation by pulsed electric fields
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735277/
https://www.ncbi.nlm.nih.gov/pubmed/26830154
http://dx.doi.org/10.1038/srep19778
work_keys_str_mv AT pilletflavien cellwallasatargetforbacteriainactivationbypulsedelectricfields
AT formosadaguececile cellwallasatargetforbacteriainactivationbypulsedelectricfields
AT baazizhouda cellwallasatargetforbacteriainactivationbypulsedelectricfields
AT dagueetienne cellwallasatargetforbacteriainactivationbypulsedelectricfields
AT rolsmariepierre cellwallasatargetforbacteriainactivationbypulsedelectricfields