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In vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography

Muscle atrophy is a widespread ill condition occurring in many diseases, which can reduce quality of life and increase morbidity and mortality. We developed a new method using non-invasive ultrasonography to measure soleus and gastrocnemius lateralis muscle atrophy in the hindlimb-unloaded rat, a we...

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Autores principales: Mele, Antonietta, Fonzino, Adriano, Rana, Francesco, Camerino, Giulia Maria, De Bellis, Michela, Conte, Elena, Giustino, Arcangela, Conte Camerino, Diana, Desaphy, Jean-François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735519/
https://www.ncbi.nlm.nih.gov/pubmed/26832124
http://dx.doi.org/10.1038/srep20061
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author Mele, Antonietta
Fonzino, Adriano
Rana, Francesco
Camerino, Giulia Maria
De Bellis, Michela
Conte, Elena
Giustino, Arcangela
Conte Camerino, Diana
Desaphy, Jean-François
author_facet Mele, Antonietta
Fonzino, Adriano
Rana, Francesco
Camerino, Giulia Maria
De Bellis, Michela
Conte, Elena
Giustino, Arcangela
Conte Camerino, Diana
Desaphy, Jean-François
author_sort Mele, Antonietta
collection PubMed
description Muscle atrophy is a widespread ill condition occurring in many diseases, which can reduce quality of life and increase morbidity and mortality. We developed a new method using non-invasive ultrasonography to measure soleus and gastrocnemius lateralis muscle atrophy in the hindlimb-unloaded rat, a well-accepted model of muscle disuse. Soleus and gastrocnemius volumes were calculated using the conventional truncated-cone method and a newly-designed sinusoidal method. For Soleus muscle, the ultrasonographic volume determined in vivo with either method was linearly correlated to the volume determined ex-vivo from excised muscles as muscle weight-to-density ratio. For both soleus and gastrocnemius muscles, a strong linear correlation was obtained between the ultrasonographic volume and the muscle fiber cross-sectional area determined ex-vivo on muscle cryosections. Thus ultrasonography allowed the longitudinal in vivo evaluation of muscle atrophy progression during hindlimb unloading. This study validates ultrasonography as a powerful method for the evaluation of rodent muscle atrophy in vivo, which would prove useful in disease models and therapeutic trials.
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spelling pubmed-47355192016-02-05 In vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography Mele, Antonietta Fonzino, Adriano Rana, Francesco Camerino, Giulia Maria De Bellis, Michela Conte, Elena Giustino, Arcangela Conte Camerino, Diana Desaphy, Jean-François Sci Rep Article Muscle atrophy is a widespread ill condition occurring in many diseases, which can reduce quality of life and increase morbidity and mortality. We developed a new method using non-invasive ultrasonography to measure soleus and gastrocnemius lateralis muscle atrophy in the hindlimb-unloaded rat, a well-accepted model of muscle disuse. Soleus and gastrocnemius volumes were calculated using the conventional truncated-cone method and a newly-designed sinusoidal method. For Soleus muscle, the ultrasonographic volume determined in vivo with either method was linearly correlated to the volume determined ex-vivo from excised muscles as muscle weight-to-density ratio. For both soleus and gastrocnemius muscles, a strong linear correlation was obtained between the ultrasonographic volume and the muscle fiber cross-sectional area determined ex-vivo on muscle cryosections. Thus ultrasonography allowed the longitudinal in vivo evaluation of muscle atrophy progression during hindlimb unloading. This study validates ultrasonography as a powerful method for the evaluation of rodent muscle atrophy in vivo, which would prove useful in disease models and therapeutic trials. Nature Publishing Group 2016-02-01 /pmc/articles/PMC4735519/ /pubmed/26832124 http://dx.doi.org/10.1038/srep20061 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mele, Antonietta
Fonzino, Adriano
Rana, Francesco
Camerino, Giulia Maria
De Bellis, Michela
Conte, Elena
Giustino, Arcangela
Conte Camerino, Diana
Desaphy, Jean-François
In vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography
title In vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography
title_full In vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography
title_fullStr In vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography
title_full_unstemmed In vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography
title_short In vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography
title_sort in vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735519/
https://www.ncbi.nlm.nih.gov/pubmed/26832124
http://dx.doi.org/10.1038/srep20061
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