Growth and Quantification of MERS‐CoV Infection

Middle East respiratory syndrome coronavirus (MERS‐CoV) is an emerging highly pathogenic respiratory virus. Although MERS‐CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS‐CoV and RNA products of replication in vitro. MERS‐CoV is able to infect a rang...

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Detalles Bibliográficos
Autores principales: Coleman, Christopher M., Frieman, Matthew B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Unit
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735735/
https://www.ncbi.nlm.nih.gov/pubmed/26344219
http://dx.doi.org/10.1002/9780471729259.mc15e02s37
Descripción
Sumario:Middle East respiratory syndrome coronavirus (MERS‐CoV) is an emerging highly pathogenic respiratory virus. Although MERS‐CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS‐CoV and RNA products of replication in vitro. MERS‐CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS‐CoV are presented. © 2015 by John Wiley & Sons, Inc.

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