Growth and Quantification of MERS‐CoV Infection

Middle East respiratory syndrome coronavirus (MERS‐CoV) is an emerging highly pathogenic respiratory virus. Although MERS‐CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS‐CoV and RNA products of replication in vitro. MERS‐CoV is able to infect a rang...

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Detalles Bibliográficos
Autores principales: Coleman, Christopher M., Frieman, Matthew B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Unit
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735735/
https://www.ncbi.nlm.nih.gov/pubmed/26344219
http://dx.doi.org/10.1002/9780471729259.mc15e02s37
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author Coleman, Christopher M.
Frieman, Matthew B.
author_facet Coleman, Christopher M.
Frieman, Matthew B.
author_sort Coleman, Christopher M.
collection PubMed
description Middle East respiratory syndrome coronavirus (MERS‐CoV) is an emerging highly pathogenic respiratory virus. Although MERS‐CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS‐CoV and RNA products of replication in vitro. MERS‐CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS‐CoV are presented. © 2015 by John Wiley & Sons, Inc.
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spelling pubmed-47357352016-05-01 Growth and Quantification of MERS‐CoV Infection Coleman, Christopher M. Frieman, Matthew B. Curr Protoc Microbiol Unit Middle East respiratory syndrome coronavirus (MERS‐CoV) is an emerging highly pathogenic respiratory virus. Although MERS‐CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS‐CoV and RNA products of replication in vitro. MERS‐CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS‐CoV are presented. © 2015 by John Wiley & Sons, Inc. John Wiley and Sons Inc. 2015-05-01 2015-11 /pmc/articles/PMC4735735/ /pubmed/26344219 http://dx.doi.org/10.1002/9780471729259.mc15e02s37 Text en Copyright © 2015 John Wiley & Sons, Inc. This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
spellingShingle Unit
Coleman, Christopher M.
Frieman, Matthew B.
Growth and Quantification of MERS‐CoV Infection
title Growth and Quantification of MERS‐CoV Infection
title_full Growth and Quantification of MERS‐CoV Infection
title_fullStr Growth and Quantification of MERS‐CoV Infection
title_full_unstemmed Growth and Quantification of MERS‐CoV Infection
title_short Growth and Quantification of MERS‐CoV Infection
title_sort growth and quantification of mers‐cov infection
topic Unit
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735735/
https://www.ncbi.nlm.nih.gov/pubmed/26344219
http://dx.doi.org/10.1002/9780471729259.mc15e02s37
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