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Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies

Helicobacter (H.) suis causes gastric pathologies in both pigs and humans. Very little is known on the metabolism of this bacterium and its impact on the host. In this study, we have revealed the importance of the glutamate-generating metabolism, as shown by a complete depletion of glutamine (Gln) i...

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Autores principales: De Bruyne, Ellen, Ducatelle, Richard, Foss, Dennis, Sanchez, Margaret, Joosten, Myrthe, Zhang, Guangzhi, Smet, Annemieke, Pasmans, Frank, Haesebrouck, Freddy, Flahou, Bram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735851/
https://www.ncbi.nlm.nih.gov/pubmed/26833404
http://dx.doi.org/10.1038/srep20169
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author De Bruyne, Ellen
Ducatelle, Richard
Foss, Dennis
Sanchez, Margaret
Joosten, Myrthe
Zhang, Guangzhi
Smet, Annemieke
Pasmans, Frank
Haesebrouck, Freddy
Flahou, Bram
author_facet De Bruyne, Ellen
Ducatelle, Richard
Foss, Dennis
Sanchez, Margaret
Joosten, Myrthe
Zhang, Guangzhi
Smet, Annemieke
Pasmans, Frank
Haesebrouck, Freddy
Flahou, Bram
author_sort De Bruyne, Ellen
collection PubMed
description Helicobacter (H.) suis causes gastric pathologies in both pigs and humans. Very little is known on the metabolism of this bacterium and its impact on the host. In this study, we have revealed the importance of the glutamate-generating metabolism, as shown by a complete depletion of glutamine (Gln) in the medium during H. suis culture. Besides Gln, H. suis can also convert glutathione (GSH) to glutamate, and both reactions are catalyzed by the H. suis γ-glutamyltranspeptidase (GGT). Both for H. pylori and H. suis, it has been hypothesized that the degradation of Gln and GSH may lead to a deficiency for the host, possibly initiating or promoting several pathologies. Therefore the in vivo effect of oral supplementation with Gln and GSH was assessed. Oral supplementation with Gln was shown to temper H. suis induced gastritis and epithelial (hyper)proliferation in Mongolian gerbils. Astonishingly, supplementation of the feed with GSH, another GGT substrate, resulted in inflammation and epithelial proliferation levels returning to baseline levels of uninfected controls. This indicates that Gln and GSH supplementation may help reducing tissue damage caused by Helicobacter infection in both humans and pigs, highlighting their potential as a supportive therapy during and after Helicobacter eradication therapy.
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spelling pubmed-47358512016-02-05 Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies De Bruyne, Ellen Ducatelle, Richard Foss, Dennis Sanchez, Margaret Joosten, Myrthe Zhang, Guangzhi Smet, Annemieke Pasmans, Frank Haesebrouck, Freddy Flahou, Bram Sci Rep Article Helicobacter (H.) suis causes gastric pathologies in both pigs and humans. Very little is known on the metabolism of this bacterium and its impact on the host. In this study, we have revealed the importance of the glutamate-generating metabolism, as shown by a complete depletion of glutamine (Gln) in the medium during H. suis culture. Besides Gln, H. suis can also convert glutathione (GSH) to glutamate, and both reactions are catalyzed by the H. suis γ-glutamyltranspeptidase (GGT). Both for H. pylori and H. suis, it has been hypothesized that the degradation of Gln and GSH may lead to a deficiency for the host, possibly initiating or promoting several pathologies. Therefore the in vivo effect of oral supplementation with Gln and GSH was assessed. Oral supplementation with Gln was shown to temper H. suis induced gastritis and epithelial (hyper)proliferation in Mongolian gerbils. Astonishingly, supplementation of the feed with GSH, another GGT substrate, resulted in inflammation and epithelial proliferation levels returning to baseline levels of uninfected controls. This indicates that Gln and GSH supplementation may help reducing tissue damage caused by Helicobacter infection in both humans and pigs, highlighting their potential as a supportive therapy during and after Helicobacter eradication therapy. Nature Publishing Group 2016-02-02 /pmc/articles/PMC4735851/ /pubmed/26833404 http://dx.doi.org/10.1038/srep20169 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
De Bruyne, Ellen
Ducatelle, Richard
Foss, Dennis
Sanchez, Margaret
Joosten, Myrthe
Zhang, Guangzhi
Smet, Annemieke
Pasmans, Frank
Haesebrouck, Freddy
Flahou, Bram
Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies
title Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies
title_full Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies
title_fullStr Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies
title_full_unstemmed Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies
title_short Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies
title_sort oral glutathione supplementation drastically reduces helicobacter-induced gastric pathologies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735851/
https://www.ncbi.nlm.nih.gov/pubmed/26833404
http://dx.doi.org/10.1038/srep20169
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