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Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis

BACKGROUND: The production and development of an effective fungicidal drug requires the identification of an essential fungal protein as a drug target. Aconitase (ACO) is a mitochondrial protein that plays a vital role in tricarboxylic acid (TCA) cycle and thus production of energy within the cell....

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Autores principales: Fateh, Roohollah, Zaini, Farideh, Kordbacheh, Parivash, Falahati, Mehraban, Rezaie, Sasan, Daie Ghazvini, Roshanak, Borhani, Nahid, Safara, Mahin, Fattahi, Azam, Kanani, Ali, Farahyar, Shirin, Bolhassani, Manzar, Heidari, Mansour
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736022/
https://www.ncbi.nlm.nih.gov/pubmed/26855741
http://dx.doi.org/10.5812/jjm.25218
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author Fateh, Roohollah
Zaini, Farideh
Kordbacheh, Parivash
Falahati, Mehraban
Rezaie, Sasan
Daie Ghazvini, Roshanak
Borhani, Nahid
Safara, Mahin
Fattahi, Azam
Kanani, Ali
Farahyar, Shirin
Bolhassani, Manzar
Heidari, Mansour
author_facet Fateh, Roohollah
Zaini, Farideh
Kordbacheh, Parivash
Falahati, Mehraban
Rezaie, Sasan
Daie Ghazvini, Roshanak
Borhani, Nahid
Safara, Mahin
Fattahi, Azam
Kanani, Ali
Farahyar, Shirin
Bolhassani, Manzar
Heidari, Mansour
author_sort Fateh, Roohollah
collection PubMed
description BACKGROUND: The production and development of an effective fungicidal drug requires the identification of an essential fungal protein as a drug target. Aconitase (ACO) is a mitochondrial protein that plays a vital role in tricarboxylic acid (TCA) cycle and thus production of energy within the cell. OBJECTIVES: The current study aimed to sequence Candida krusei ACO gene and determine any amino acid residue differences between human and fungal aconitases to obtain selective inhibition. MATERIALS AND METHODS: Candida krusei (ATCC: 6258) aconitase gene was determined by 5’Rapid Amplification of cDNA Ends (RACE) method and degenerate Polymerase Chain Reaction (PCR) and analyzed using bioinformatics softwares. RESULTS: One thousand-four hundred-nineteen nucleotide of C. krusei aconitase gene were clarified and submitted in Genbank as a partial sequence and then taxonomic location of C. krusei was determined by nucleotide and amino acid sequences of this gene. The comparison of nucleotide and amino acid sequences of Candida species ACO genes showed that C. krusei possessed characteristic sequences. No significant differences were observed between C. krusei and human aconitases within the active site amino acid residues. CONCLUSIONS: Results of the current study indicated that aconitase was not a suitable target to design new anti-fungal drugs that selectively block this enzyme.
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spelling pubmed-47360222016-02-05 Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis Fateh, Roohollah Zaini, Farideh Kordbacheh, Parivash Falahati, Mehraban Rezaie, Sasan Daie Ghazvini, Roshanak Borhani, Nahid Safara, Mahin Fattahi, Azam Kanani, Ali Farahyar, Shirin Bolhassani, Manzar Heidari, Mansour Jundishapur J Microbiol Research Article BACKGROUND: The production and development of an effective fungicidal drug requires the identification of an essential fungal protein as a drug target. Aconitase (ACO) is a mitochondrial protein that plays a vital role in tricarboxylic acid (TCA) cycle and thus production of energy within the cell. OBJECTIVES: The current study aimed to sequence Candida krusei ACO gene and determine any amino acid residue differences between human and fungal aconitases to obtain selective inhibition. MATERIALS AND METHODS: Candida krusei (ATCC: 6258) aconitase gene was determined by 5’Rapid Amplification of cDNA Ends (RACE) method and degenerate Polymerase Chain Reaction (PCR) and analyzed using bioinformatics softwares. RESULTS: One thousand-four hundred-nineteen nucleotide of C. krusei aconitase gene were clarified and submitted in Genbank as a partial sequence and then taxonomic location of C. krusei was determined by nucleotide and amino acid sequences of this gene. The comparison of nucleotide and amino acid sequences of Candida species ACO genes showed that C. krusei possessed characteristic sequences. No significant differences were observed between C. krusei and human aconitases within the active site amino acid residues. CONCLUSIONS: Results of the current study indicated that aconitase was not a suitable target to design new anti-fungal drugs that selectively block this enzyme. Kowsar 2015-11-21 /pmc/articles/PMC4736022/ /pubmed/26855741 http://dx.doi.org/10.5812/jjm.25218 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Fateh, Roohollah
Zaini, Farideh
Kordbacheh, Parivash
Falahati, Mehraban
Rezaie, Sasan
Daie Ghazvini, Roshanak
Borhani, Nahid
Safara, Mahin
Fattahi, Azam
Kanani, Ali
Farahyar, Shirin
Bolhassani, Manzar
Heidari, Mansour
Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis
title Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis
title_full Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis
title_fullStr Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis
title_full_unstemmed Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis
title_short Identification and Sequencing of Candida krusei Aconitate Hydratase Gene Using Rapid Amplification of cDNA Ends Method and Phylogenetic Analysis
title_sort identification and sequencing of candida krusei aconitate hydratase gene using rapid amplification of cdna ends method and phylogenetic analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736022/
https://www.ncbi.nlm.nih.gov/pubmed/26855741
http://dx.doi.org/10.5812/jjm.25218
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