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A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard
BACKGROUND: The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the construction of transcriptional gene circuits, many of which depend on the technical abili...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736081/ https://www.ncbi.nlm.nih.gov/pubmed/26839579 http://dx.doi.org/10.1186/s13007-016-0101-2 |