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ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First,...

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Detalles Bibliográficos
Autores principales:	Yoshimi, Kazuto, Kunihiro, Yayoi, Kaneko, Takehito, Nagahora, Hitoshi, Voigt, Birger, Mashimo, Tomoji
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736110/ https://www.ncbi.nlm.nih.gov/pubmed/26786405 http://dx.doi.org/10.1038/ncomms10431

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