Cargando…
End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis
BACKGROUND: Synthetic biology aims to engineer biological systems for desired behaviors. The construction of these systems can be complex, often requiring genetic reprogramming, extensive de novo DNA synthesis, and functional screening. RESULTS: Herein, we present a programmable, multipurpose microf...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736182/ https://www.ncbi.nlm.nih.gov/pubmed/26839585 http://dx.doi.org/10.1186/s13036-016-0024-5 |
_version_ | 1782413227174395904 |
---|---|
author | Linshiz, Gregory Jensen, Erik Stawski, Nina Bi, Changhao Elsbree, Nick Jiao, Hong Kim, Jungkyu Mathies, Richard Keasling, Jay D. Hillson, Nathan J. |
author_facet | Linshiz, Gregory Jensen, Erik Stawski, Nina Bi, Changhao Elsbree, Nick Jiao, Hong Kim, Jungkyu Mathies, Richard Keasling, Jay D. Hillson, Nathan J. |
author_sort | Linshiz, Gregory |
collection | PubMed |
description | BACKGROUND: Synthetic biology aims to engineer biological systems for desired behaviors. The construction of these systems can be complex, often requiring genetic reprogramming, extensive de novo DNA synthesis, and functional screening. RESULTS: Herein, we present a programmable, multipurpose microfluidic platform and associated software and apply the platform to major steps of the synthetic biology research cycle: design, construction, testing, and analysis. We show the platform’s capabilities for multiple automated DNA assembly methods, including a new method for Isothermal Hierarchical DNA Construction, and for Escherichia coli and Saccharomyces cerevisiae transformation. The platform enables the automated control of cellular growth, gene expression induction, and proteogenic and metabolic output analysis. CONCLUSIONS: Taken together, we demonstrate the microfluidic platform’s potential to provide end-to-end solutions for synthetic biology research, from design to functional analysis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13036-016-0024-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4736182 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47361822016-02-03 End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis Linshiz, Gregory Jensen, Erik Stawski, Nina Bi, Changhao Elsbree, Nick Jiao, Hong Kim, Jungkyu Mathies, Richard Keasling, Jay D. Hillson, Nathan J. J Biol Eng Research BACKGROUND: Synthetic biology aims to engineer biological systems for desired behaviors. The construction of these systems can be complex, often requiring genetic reprogramming, extensive de novo DNA synthesis, and functional screening. RESULTS: Herein, we present a programmable, multipurpose microfluidic platform and associated software and apply the platform to major steps of the synthetic biology research cycle: design, construction, testing, and analysis. We show the platform’s capabilities for multiple automated DNA assembly methods, including a new method for Isothermal Hierarchical DNA Construction, and for Escherichia coli and Saccharomyces cerevisiae transformation. The platform enables the automated control of cellular growth, gene expression induction, and proteogenic and metabolic output analysis. CONCLUSIONS: Taken together, we demonstrate the microfluidic platform’s potential to provide end-to-end solutions for synthetic biology research, from design to functional analysis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13036-016-0024-5) contains supplementary material, which is available to authorized users. BioMed Central 2016-02-02 /pmc/articles/PMC4736182/ /pubmed/26839585 http://dx.doi.org/10.1186/s13036-016-0024-5 Text en © Linshiz et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Linshiz, Gregory Jensen, Erik Stawski, Nina Bi, Changhao Elsbree, Nick Jiao, Hong Kim, Jungkyu Mathies, Richard Keasling, Jay D. Hillson, Nathan J. End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis |
title | End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis |
title_full | End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis |
title_fullStr | End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis |
title_full_unstemmed | End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis |
title_short | End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis |
title_sort | end-to-end automated microfluidic platform for synthetic biology: from design to functional analysis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736182/ https://www.ncbi.nlm.nih.gov/pubmed/26839585 http://dx.doi.org/10.1186/s13036-016-0024-5 |
work_keys_str_mv | AT linshizgregory endtoendautomatedmicrofluidicplatformforsyntheticbiologyfromdesigntofunctionalanalysis AT jensenerik endtoendautomatedmicrofluidicplatformforsyntheticbiologyfromdesigntofunctionalanalysis AT stawskinina endtoendautomatedmicrofluidicplatformforsyntheticbiologyfromdesigntofunctionalanalysis AT bichanghao endtoendautomatedmicrofluidicplatformforsyntheticbiologyfromdesigntofunctionalanalysis AT elsbreenick endtoendautomatedmicrofluidicplatformforsyntheticbiologyfromdesigntofunctionalanalysis AT jiaohong endtoendautomatedmicrofluidicplatformforsyntheticbiologyfromdesigntofunctionalanalysis AT kimjungkyu endtoendautomatedmicrofluidicplatformforsyntheticbiologyfromdesigntofunctionalanalysis AT mathiesrichard endtoendautomatedmicrofluidicplatformforsyntheticbiologyfromdesigntofunctionalanalysis AT keaslingjayd endtoendautomatedmicrofluidicplatformforsyntheticbiologyfromdesigntofunctionalanalysis AT hillsonnathanj endtoendautomatedmicrofluidicplatformforsyntheticbiologyfromdesigntofunctionalanalysis |