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Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation

BACKGROUND: Co-production of chemicals from lignocellulosic biomass alongside fuels holds promise for improving the economic outlook of integrated biorefineries. In current biochemical conversion processes that use thermochemical pretreatment and enzymatic hydrolysis, fractionation of hemicellulose-...

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Autores principales: Salvachúa, Davinia, Mohagheghi, Ali, Smith, Holly, Bradfield, Michael F. A., Nicol, Willie, Black, Brenna A., Biddy, Mary J., Dowe, Nancy, Beckham, Gregg T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736274/
https://www.ncbi.nlm.nih.gov/pubmed/26839591
http://dx.doi.org/10.1186/s13068-016-0425-1
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author Salvachúa, Davinia
Mohagheghi, Ali
Smith, Holly
Bradfield, Michael F. A.
Nicol, Willie
Black, Brenna A.
Biddy, Mary J.
Dowe, Nancy
Beckham, Gregg T.
author_facet Salvachúa, Davinia
Mohagheghi, Ali
Smith, Holly
Bradfield, Michael F. A.
Nicol, Willie
Black, Brenna A.
Biddy, Mary J.
Dowe, Nancy
Beckham, Gregg T.
author_sort Salvachúa, Davinia
collection PubMed
description BACKGROUND: Co-production of chemicals from lignocellulosic biomass alongside fuels holds promise for improving the economic outlook of integrated biorefineries. In current biochemical conversion processes that use thermochemical pretreatment and enzymatic hydrolysis, fractionation of hemicellulose-derived and cellulose-derived sugar streams is possible using hydrothermal or dilute acid pretreatment (DAP), which then offers a route to parallel trains for fuel and chemical production from xylose- and glucose-enriched streams. Succinic acid (SA) is a co-product of particular interest in biorefineries because it could potentially displace petroleum-derived chemicals and polymer precursors for myriad applications. However, SA production from biomass-derived hydrolysates has not yet been fully explored or developed. RESULTS: Here, we employ Actinobacillus succinogenes 130Z to produce succinate in batch fermentations from various substrates including (1) pure sugars to quantify substrate inhibition, (2) from mock hydrolysates similar to those from DAP containing single putative inhibitors, and (3) using the hydrolysate derived from two pilot-scale pretreatments: first, a mild alkaline wash (deacetylation) followed by DAP, and secondly a single DAP step, both with corn stover. These latter streams are both rich in xylose and contain different levels of inhibitors such as acetate, sugar dehydration products (furfural, 5-hydroxymethylfurfural), and lignin-derived products (ferulate, p-coumarate). In batch fermentations, we quantify succinate and co-product (acetate and formate) titers as well as succinate yields and productivities. We demonstrate yields of 0.74 g succinate/g sugars and 42.8 g/L succinate from deacetylated DAP hydrolysate, achieving maximum productivities of up to 1.27 g/L-h. Moreover, A. succinogenes is shown to detoxify furfural via reduction to furfuryl alcohol, although an initial lag in succinate production is observed when furans are present. Acetate seems to be the main inhibitor for this bacterium present in biomass hydrolysates. CONCLUSION: Overall, these results demonstrate that biomass-derived, xylose-enriched hydrolysates result in similar yields and titers but lower productivities compared to clean sugar streams, which can likely be improved via fermentation process developments and metabolic engineering. Overall, this study comprehensively examines the behavior of A. succinogenes on xylose-enriched hydrolysates on an industrially relevant, lignocellulosic feedstock, which will pave the way for future work toward eventual SA production in an integrated biorefinery. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-016-0425-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-47362742016-02-03 Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation Salvachúa, Davinia Mohagheghi, Ali Smith, Holly Bradfield, Michael F. A. Nicol, Willie Black, Brenna A. Biddy, Mary J. Dowe, Nancy Beckham, Gregg T. Biotechnol Biofuels Research BACKGROUND: Co-production of chemicals from lignocellulosic biomass alongside fuels holds promise for improving the economic outlook of integrated biorefineries. In current biochemical conversion processes that use thermochemical pretreatment and enzymatic hydrolysis, fractionation of hemicellulose-derived and cellulose-derived sugar streams is possible using hydrothermal or dilute acid pretreatment (DAP), which then offers a route to parallel trains for fuel and chemical production from xylose- and glucose-enriched streams. Succinic acid (SA) is a co-product of particular interest in biorefineries because it could potentially displace petroleum-derived chemicals and polymer precursors for myriad applications. However, SA production from biomass-derived hydrolysates has not yet been fully explored or developed. RESULTS: Here, we employ Actinobacillus succinogenes 130Z to produce succinate in batch fermentations from various substrates including (1) pure sugars to quantify substrate inhibition, (2) from mock hydrolysates similar to those from DAP containing single putative inhibitors, and (3) using the hydrolysate derived from two pilot-scale pretreatments: first, a mild alkaline wash (deacetylation) followed by DAP, and secondly a single DAP step, both with corn stover. These latter streams are both rich in xylose and contain different levels of inhibitors such as acetate, sugar dehydration products (furfural, 5-hydroxymethylfurfural), and lignin-derived products (ferulate, p-coumarate). In batch fermentations, we quantify succinate and co-product (acetate and formate) titers as well as succinate yields and productivities. We demonstrate yields of 0.74 g succinate/g sugars and 42.8 g/L succinate from deacetylated DAP hydrolysate, achieving maximum productivities of up to 1.27 g/L-h. Moreover, A. succinogenes is shown to detoxify furfural via reduction to furfuryl alcohol, although an initial lag in succinate production is observed when furans are present. Acetate seems to be the main inhibitor for this bacterium present in biomass hydrolysates. CONCLUSION: Overall, these results demonstrate that biomass-derived, xylose-enriched hydrolysates result in similar yields and titers but lower productivities compared to clean sugar streams, which can likely be improved via fermentation process developments and metabolic engineering. Overall, this study comprehensively examines the behavior of A. succinogenes on xylose-enriched hydrolysates on an industrially relevant, lignocellulosic feedstock, which will pave the way for future work toward eventual SA production in an integrated biorefinery. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-016-0425-1) contains supplementary material, which is available to authorized users. BioMed Central 2016-02-02 /pmc/articles/PMC4736274/ /pubmed/26839591 http://dx.doi.org/10.1186/s13068-016-0425-1 Text en © Salvachúa et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Salvachúa, Davinia
Mohagheghi, Ali
Smith, Holly
Bradfield, Michael F. A.
Nicol, Willie
Black, Brenna A.
Biddy, Mary J.
Dowe, Nancy
Beckham, Gregg T.
Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation
title Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation
title_full Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation
title_fullStr Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation
title_full_unstemmed Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation
title_short Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation
title_sort succinic acid production on xylose-enriched biorefinery streams by actinobacillus succinogenes in batch fermentation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736274/
https://www.ncbi.nlm.nih.gov/pubmed/26839591
http://dx.doi.org/10.1186/s13068-016-0425-1
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