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Morphometric study of the testis and reproductive tract (including sperm granuloma) after vasectomy in mature rats

By utilizing the rabbit model, previous studies have found good evidence indicating that vasectomy-induced spermatogenic damage is pressure-mediated: the damage occurs when the occluded reproductive tract is unable to accommodate additional spermatozoa produced by the testis. More studies with the m...

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Autores principales: Ma, Li, Guo, Yang, Yuan, Yong, Li, Yu-Gen, Deng, Xian-Zhong, Yang, Zheng-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736359/
https://www.ncbi.nlm.nih.gov/pubmed/25791731
http://dx.doi.org/10.4103/1008-682X.150038
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author Ma, Li
Guo, Yang
Yuan, Yong
Li, Yu-Gen
Deng, Xian-Zhong
Yang, Zheng-Wei
author_facet Ma, Li
Guo, Yang
Yuan, Yong
Li, Yu-Gen
Deng, Xian-Zhong
Yang, Zheng-Wei
author_sort Ma, Li
collection PubMed
description By utilizing the rabbit model, previous studies have found good evidence indicating that vasectomy-induced spermatogenic damage is pressure-mediated: the damage occurs when the occluded reproductive tract is unable to accommodate additional spermatozoa produced by the testis. More studies with the more commonly used rat model have shown, however, controversial results on whether and why the damage occurs. In this study, 12 mature male Sprague-Dawley rats were subjected to unilateral vasectomy: double ligation (without severing) of the vas deferens exposed via a small inguinal incision; 37 days after the operation, the testes, epididymides, vasa deferentia (juxta-epididymal segments), and sperm granulomas (at the vasectomy site) were removed to obtain methacrylate resin-embedded sections and morphometric studies carried out with light microscopy. Marked spermatogenic damage with spermatids and spermatocytes depleted in the seminiferous epithelium in 43% of the seminiferous tubule profiles was demonstrated in 5 of the 12 testes on the vasectomized side, and the damage was associated with smaller or absent sperm granulomas; in the other 7 testes with essentially normal spermatogenesis, there was an increase (by 111% on average) in the volume of the tubule lumen, associated with larger granulomas or granulomas containing more spermatozoa. There was an overall increase (by 66%) in the thickness of the rete testis in the 12 testes; the epididymis or vas deferens showed no distension. It seems therefore that the spermatogenic damage induced by vasectomy in rats is pressure-mediated as well, and that variation in the damage depends mainly on the postoperative development of the sperm granuloma.
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spelling pubmed-47363592016-02-04 Morphometric study of the testis and reproductive tract (including sperm granuloma) after vasectomy in mature rats Ma, Li Guo, Yang Yuan, Yong Li, Yu-Gen Deng, Xian-Zhong Yang, Zheng-Wei Asian J Androl Original Article By utilizing the rabbit model, previous studies have found good evidence indicating that vasectomy-induced spermatogenic damage is pressure-mediated: the damage occurs when the occluded reproductive tract is unable to accommodate additional spermatozoa produced by the testis. More studies with the more commonly used rat model have shown, however, controversial results on whether and why the damage occurs. In this study, 12 mature male Sprague-Dawley rats were subjected to unilateral vasectomy: double ligation (without severing) of the vas deferens exposed via a small inguinal incision; 37 days after the operation, the testes, epididymides, vasa deferentia (juxta-epididymal segments), and sperm granulomas (at the vasectomy site) were removed to obtain methacrylate resin-embedded sections and morphometric studies carried out with light microscopy. Marked spermatogenic damage with spermatids and spermatocytes depleted in the seminiferous epithelium in 43% of the seminiferous tubule profiles was demonstrated in 5 of the 12 testes on the vasectomized side, and the damage was associated with smaller or absent sperm granulomas; in the other 7 testes with essentially normal spermatogenesis, there was an increase (by 111% on average) in the volume of the tubule lumen, associated with larger granulomas or granulomas containing more spermatozoa. There was an overall increase (by 66%) in the thickness of the rete testis in the 12 testes; the epididymis or vas deferens showed no distension. It seems therefore that the spermatogenic damage induced by vasectomy in rats is pressure-mediated as well, and that variation in the damage depends mainly on the postoperative development of the sperm granuloma. Medknow Publications & Media Pvt Ltd 2016 2015-03-17 /pmc/articles/PMC4736359/ /pubmed/25791731 http://dx.doi.org/10.4103/1008-682X.150038 Text en Copyright: © Asian Journal of Andrology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution NonCommercial ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Ma, Li
Guo, Yang
Yuan, Yong
Li, Yu-Gen
Deng, Xian-Zhong
Yang, Zheng-Wei
Morphometric study of the testis and reproductive tract (including sperm granuloma) after vasectomy in mature rats
title Morphometric study of the testis and reproductive tract (including sperm granuloma) after vasectomy in mature rats
title_full Morphometric study of the testis and reproductive tract (including sperm granuloma) after vasectomy in mature rats
title_fullStr Morphometric study of the testis and reproductive tract (including sperm granuloma) after vasectomy in mature rats
title_full_unstemmed Morphometric study of the testis and reproductive tract (including sperm granuloma) after vasectomy in mature rats
title_short Morphometric study of the testis and reproductive tract (including sperm granuloma) after vasectomy in mature rats
title_sort morphometric study of the testis and reproductive tract (including sperm granuloma) after vasectomy in mature rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736359/
https://www.ncbi.nlm.nih.gov/pubmed/25791731
http://dx.doi.org/10.4103/1008-682X.150038
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