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Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability
Increasing the success rate of rotator cuff healing remains tremendous challenge. Among many approaches, the possibility of activating resident stem cells in situ, without the need to isolate them from biopsies, could represent valuable therapeutic strategy. Along this line, it has been recently dem...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736573/ https://www.ncbi.nlm.nih.gov/pubmed/27057170 http://dx.doi.org/10.1155/2016/4373410 |
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author | Randelli, Pietro Menon, Alessandra Ragone, Vincenza Creo, Pasquale Bergante, Sonia Randelli, Filippo De Girolamo, Laura Alfieri Montrasio, Umberto Banfi, Giuseppe Cabitza, Paolo Tettamanti, Guido Anastasia, Luigi |
author_facet | Randelli, Pietro Menon, Alessandra Ragone, Vincenza Creo, Pasquale Bergante, Sonia Randelli, Filippo De Girolamo, Laura Alfieri Montrasio, Umberto Banfi, Giuseppe Cabitza, Paolo Tettamanti, Guido Anastasia, Luigi |
author_sort | Randelli, Pietro |
collection | PubMed |
description | Increasing the success rate of rotator cuff healing remains tremendous challenge. Among many approaches, the possibility of activating resident stem cells in situ, without the need to isolate them from biopsies, could represent valuable therapeutic strategy. Along this line, it has been recently demonstrated that lipoaspirate product, Lipogems, contains and produces growth-factors that may activate resident stem cells. In this study, human tendon stem cells (hTSCs) from the rotator cuff were cocultured in a transwell system with the Lipogems lipoaspirate product and compared to control untreated cells in terms of cell proliferation, morphology, stem cell marker and VEGF expression, and differentiation and migration capabilities. Results showed that the Lipogems product significantly increases the proliferation rate of hTSCs without altering their stemness and differentiation capability. Moreover, treated cells increase the expression of VEGF, which is crucial for the neovascularization of the tissue during the healing process. Overall, this study supports that directly activating hTSCs with the Lipogems lipoaspirate could represent a new practical therapeutic approach. In fact, obtaining a lipoaspirate is easier, safer, and more cost-effective than harvesting cells from tendon or bone marrow biopsies, expanding them in GMP facility and then reinjecting them in the patient. |
format | Online Article Text |
id | pubmed-4736573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-47365732016-04-07 Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability Randelli, Pietro Menon, Alessandra Ragone, Vincenza Creo, Pasquale Bergante, Sonia Randelli, Filippo De Girolamo, Laura Alfieri Montrasio, Umberto Banfi, Giuseppe Cabitza, Paolo Tettamanti, Guido Anastasia, Luigi Stem Cells Int Research Article Increasing the success rate of rotator cuff healing remains tremendous challenge. Among many approaches, the possibility of activating resident stem cells in situ, without the need to isolate them from biopsies, could represent valuable therapeutic strategy. Along this line, it has been recently demonstrated that lipoaspirate product, Lipogems, contains and produces growth-factors that may activate resident stem cells. In this study, human tendon stem cells (hTSCs) from the rotator cuff were cocultured in a transwell system with the Lipogems lipoaspirate product and compared to control untreated cells in terms of cell proliferation, morphology, stem cell marker and VEGF expression, and differentiation and migration capabilities. Results showed that the Lipogems product significantly increases the proliferation rate of hTSCs without altering their stemness and differentiation capability. Moreover, treated cells increase the expression of VEGF, which is crucial for the neovascularization of the tissue during the healing process. Overall, this study supports that directly activating hTSCs with the Lipogems lipoaspirate could represent a new practical therapeutic approach. In fact, obtaining a lipoaspirate is easier, safer, and more cost-effective than harvesting cells from tendon or bone marrow biopsies, expanding them in GMP facility and then reinjecting them in the patient. Hindawi Publishing Corporation 2016 2016-01-06 /pmc/articles/PMC4736573/ /pubmed/27057170 http://dx.doi.org/10.1155/2016/4373410 Text en Copyright © 2016 Pietro Randelli et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Randelli, Pietro Menon, Alessandra Ragone, Vincenza Creo, Pasquale Bergante, Sonia Randelli, Filippo De Girolamo, Laura Alfieri Montrasio, Umberto Banfi, Giuseppe Cabitza, Paolo Tettamanti, Guido Anastasia, Luigi Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability |
title | Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability |
title_full | Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability |
title_fullStr | Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability |
title_full_unstemmed | Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability |
title_short | Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability |
title_sort | lipogems product treatment increases the proliferation rate of human tendon stem cells without affecting their stemness and differentiation capability |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736573/ https://www.ncbi.nlm.nih.gov/pubmed/27057170 http://dx.doi.org/10.1155/2016/4373410 |
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