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Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability

Increasing the success rate of rotator cuff healing remains tremendous challenge. Among many approaches, the possibility of activating resident stem cells in situ, without the need to isolate them from biopsies, could represent valuable therapeutic strategy. Along this line, it has been recently dem...

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Autores principales: Randelli, Pietro, Menon, Alessandra, Ragone, Vincenza, Creo, Pasquale, Bergante, Sonia, Randelli, Filippo, De Girolamo, Laura, Alfieri Montrasio, Umberto, Banfi, Giuseppe, Cabitza, Paolo, Tettamanti, Guido, Anastasia, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736573/
https://www.ncbi.nlm.nih.gov/pubmed/27057170
http://dx.doi.org/10.1155/2016/4373410
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author Randelli, Pietro
Menon, Alessandra
Ragone, Vincenza
Creo, Pasquale
Bergante, Sonia
Randelli, Filippo
De Girolamo, Laura
Alfieri Montrasio, Umberto
Banfi, Giuseppe
Cabitza, Paolo
Tettamanti, Guido
Anastasia, Luigi
author_facet Randelli, Pietro
Menon, Alessandra
Ragone, Vincenza
Creo, Pasquale
Bergante, Sonia
Randelli, Filippo
De Girolamo, Laura
Alfieri Montrasio, Umberto
Banfi, Giuseppe
Cabitza, Paolo
Tettamanti, Guido
Anastasia, Luigi
author_sort Randelli, Pietro
collection PubMed
description Increasing the success rate of rotator cuff healing remains tremendous challenge. Among many approaches, the possibility of activating resident stem cells in situ, without the need to isolate them from biopsies, could represent valuable therapeutic strategy. Along this line, it has been recently demonstrated that lipoaspirate product, Lipogems, contains and produces growth-factors that may activate resident stem cells. In this study, human tendon stem cells (hTSCs) from the rotator cuff were cocultured in a transwell system with the Lipogems lipoaspirate product and compared to control untreated cells in terms of cell proliferation, morphology, stem cell marker and VEGF expression, and differentiation and migration capabilities. Results showed that the Lipogems product significantly increases the proliferation rate of hTSCs without altering their stemness and differentiation capability. Moreover, treated cells increase the expression of VEGF, which is crucial for the neovascularization of the tissue during the healing process. Overall, this study supports that directly activating hTSCs with the Lipogems lipoaspirate could represent a new practical therapeutic approach. In fact, obtaining a lipoaspirate is easier, safer, and more cost-effective than harvesting cells from tendon or bone marrow biopsies, expanding them in GMP facility and then reinjecting them in the patient.
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spelling pubmed-47365732016-04-07 Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability Randelli, Pietro Menon, Alessandra Ragone, Vincenza Creo, Pasquale Bergante, Sonia Randelli, Filippo De Girolamo, Laura Alfieri Montrasio, Umberto Banfi, Giuseppe Cabitza, Paolo Tettamanti, Guido Anastasia, Luigi Stem Cells Int Research Article Increasing the success rate of rotator cuff healing remains tremendous challenge. Among many approaches, the possibility of activating resident stem cells in situ, without the need to isolate them from biopsies, could represent valuable therapeutic strategy. Along this line, it has been recently demonstrated that lipoaspirate product, Lipogems, contains and produces growth-factors that may activate resident stem cells. In this study, human tendon stem cells (hTSCs) from the rotator cuff were cocultured in a transwell system with the Lipogems lipoaspirate product and compared to control untreated cells in terms of cell proliferation, morphology, stem cell marker and VEGF expression, and differentiation and migration capabilities. Results showed that the Lipogems product significantly increases the proliferation rate of hTSCs without altering their stemness and differentiation capability. Moreover, treated cells increase the expression of VEGF, which is crucial for the neovascularization of the tissue during the healing process. Overall, this study supports that directly activating hTSCs with the Lipogems lipoaspirate could represent a new practical therapeutic approach. In fact, obtaining a lipoaspirate is easier, safer, and more cost-effective than harvesting cells from tendon or bone marrow biopsies, expanding them in GMP facility and then reinjecting them in the patient. Hindawi Publishing Corporation 2016 2016-01-06 /pmc/articles/PMC4736573/ /pubmed/27057170 http://dx.doi.org/10.1155/2016/4373410 Text en Copyright © 2016 Pietro Randelli et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Randelli, Pietro
Menon, Alessandra
Ragone, Vincenza
Creo, Pasquale
Bergante, Sonia
Randelli, Filippo
De Girolamo, Laura
Alfieri Montrasio, Umberto
Banfi, Giuseppe
Cabitza, Paolo
Tettamanti, Guido
Anastasia, Luigi
Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability
title Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability
title_full Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability
title_fullStr Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability
title_full_unstemmed Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability
title_short Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability
title_sort lipogems product treatment increases the proliferation rate of human tendon stem cells without affecting their stemness and differentiation capability
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736573/
https://www.ncbi.nlm.nih.gov/pubmed/27057170
http://dx.doi.org/10.1155/2016/4373410
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