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Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats
Background. The incidence of metabolic syndrome co-existing with diabetes mellitus is on the rise globally. Objective. The present study was designed to develop a unique animal model that will mimic the pathological features seen in individuals with diabetes and metabolic syndrome, suitable for phar...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736803/ https://www.ncbi.nlm.nih.gov/pubmed/26880906 http://dx.doi.org/10.1155/2016/9463476 |
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author | Suman, Rajesh Kumar Ray Mohanty, Ipseeta Borde, Manjusha K. Maheshwari, Ujwala Deshmukh, Y. A. |
author_facet | Suman, Rajesh Kumar Ray Mohanty, Ipseeta Borde, Manjusha K. Maheshwari, Ujwala Deshmukh, Y. A. |
author_sort | Suman, Rajesh Kumar |
collection | PubMed |
description | Background. The incidence of metabolic syndrome co-existing with diabetes mellitus is on the rise globally. Objective. The present study was designed to develop a unique animal model that will mimic the pathological features seen in individuals with diabetes and metabolic syndrome, suitable for pharmacological screening of drugs. Materials and Methods. A combination of High-Fat Diet (HFD) and low dose of streptozotocin (STZ) at 30, 35, and 40 mg/kg was used to induce metabolic syndrome in the setting of diabetes mellitus in Wistar rats. Results. The 40 mg/kg STZ produced sustained hyperglycemia and the dose was thus selected for the study to induce diabetes mellitus. Various components of metabolic syndrome such as dyslipidemia {(increased triglyceride, total cholesterol, LDL cholesterol, and decreased HDL cholesterol)}, diabetes mellitus (blood glucose, HbA1c, serum insulin, and C-peptide), and hypertension {systolic blood pressure} were mimicked in the developed model of metabolic syndrome co-existing with diabetes mellitus. In addition to significant cardiac injury, atherogenic index, inflammation (hs-CRP), decline in hepatic and renal function were observed in the HF-DC group when compared to NC group rats. The histopathological assessment confirmed presence of edema, necrosis, and inflammation in heart, pancreas, liver, and kidney of HF-DC group as compared to NC. Conclusion. The present study has developed a unique rodent model of metabolic syndrome, with diabetes as an essential component. |
format | Online Article Text |
id | pubmed-4736803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-47368032016-02-15 Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats Suman, Rajesh Kumar Ray Mohanty, Ipseeta Borde, Manjusha K. Maheshwari, Ujwala Deshmukh, Y. A. Adv Pharmacol Sci Research Article Background. The incidence of metabolic syndrome co-existing with diabetes mellitus is on the rise globally. Objective. The present study was designed to develop a unique animal model that will mimic the pathological features seen in individuals with diabetes and metabolic syndrome, suitable for pharmacological screening of drugs. Materials and Methods. A combination of High-Fat Diet (HFD) and low dose of streptozotocin (STZ) at 30, 35, and 40 mg/kg was used to induce metabolic syndrome in the setting of diabetes mellitus in Wistar rats. Results. The 40 mg/kg STZ produced sustained hyperglycemia and the dose was thus selected for the study to induce diabetes mellitus. Various components of metabolic syndrome such as dyslipidemia {(increased triglyceride, total cholesterol, LDL cholesterol, and decreased HDL cholesterol)}, diabetes mellitus (blood glucose, HbA1c, serum insulin, and C-peptide), and hypertension {systolic blood pressure} were mimicked in the developed model of metabolic syndrome co-existing with diabetes mellitus. In addition to significant cardiac injury, atherogenic index, inflammation (hs-CRP), decline in hepatic and renal function were observed in the HF-DC group when compared to NC group rats. The histopathological assessment confirmed presence of edema, necrosis, and inflammation in heart, pancreas, liver, and kidney of HF-DC group as compared to NC. Conclusion. The present study has developed a unique rodent model of metabolic syndrome, with diabetes as an essential component. Hindawi Publishing Corporation 2016 2016-01-06 /pmc/articles/PMC4736803/ /pubmed/26880906 http://dx.doi.org/10.1155/2016/9463476 Text en Copyright © 2016 Rajesh Kumar Suman et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Suman, Rajesh Kumar Ray Mohanty, Ipseeta Borde, Manjusha K. Maheshwari, Ujwala Deshmukh, Y. A. Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats |
title | Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats |
title_full | Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats |
title_fullStr | Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats |
title_full_unstemmed | Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats |
title_short | Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats |
title_sort | development of an experimental model of diabetes co-existing with metabolic syndrome in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736803/ https://www.ncbi.nlm.nih.gov/pubmed/26880906 http://dx.doi.org/10.1155/2016/9463476 |
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