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Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats

Background. The incidence of metabolic syndrome co-existing with diabetes mellitus is on the rise globally. Objective. The present study was designed to develop a unique animal model that will mimic the pathological features seen in individuals with diabetes and metabolic syndrome, suitable for phar...

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Autores principales: Suman, Rajesh Kumar, Ray Mohanty, Ipseeta, Borde, Manjusha K., Maheshwari, Ujwala, Deshmukh, Y. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736803/
https://www.ncbi.nlm.nih.gov/pubmed/26880906
http://dx.doi.org/10.1155/2016/9463476
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author Suman, Rajesh Kumar
Ray Mohanty, Ipseeta
Borde, Manjusha K.
Maheshwari, Ujwala
Deshmukh, Y. A.
author_facet Suman, Rajesh Kumar
Ray Mohanty, Ipseeta
Borde, Manjusha K.
Maheshwari, Ujwala
Deshmukh, Y. A.
author_sort Suman, Rajesh Kumar
collection PubMed
description Background. The incidence of metabolic syndrome co-existing with diabetes mellitus is on the rise globally. Objective. The present study was designed to develop a unique animal model that will mimic the pathological features seen in individuals with diabetes and metabolic syndrome, suitable for pharmacological screening of drugs. Materials and Methods. A combination of High-Fat Diet (HFD) and low dose of streptozotocin (STZ) at 30, 35, and 40 mg/kg was used to induce metabolic syndrome in the setting of diabetes mellitus in Wistar rats. Results. The 40 mg/kg STZ produced sustained hyperglycemia and the dose was thus selected for the study to induce diabetes mellitus. Various components of metabolic syndrome such as dyslipidemia {(increased triglyceride, total cholesterol, LDL cholesterol, and decreased HDL cholesterol)}, diabetes mellitus (blood glucose, HbA1c, serum insulin, and C-peptide), and hypertension {systolic blood pressure} were mimicked in the developed model of metabolic syndrome co-existing with diabetes mellitus. In addition to significant cardiac injury, atherogenic index, inflammation (hs-CRP), decline in hepatic and renal function were observed in the HF-DC group when compared to NC group rats. The histopathological assessment confirmed presence of edema, necrosis, and inflammation in heart, pancreas, liver, and kidney of HF-DC group as compared to NC. Conclusion. The present study has developed a unique rodent model of metabolic syndrome, with diabetes as an essential component.
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spelling pubmed-47368032016-02-15 Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats Suman, Rajesh Kumar Ray Mohanty, Ipseeta Borde, Manjusha K. Maheshwari, Ujwala Deshmukh, Y. A. Adv Pharmacol Sci Research Article Background. The incidence of metabolic syndrome co-existing with diabetes mellitus is on the rise globally. Objective. The present study was designed to develop a unique animal model that will mimic the pathological features seen in individuals with diabetes and metabolic syndrome, suitable for pharmacological screening of drugs. Materials and Methods. A combination of High-Fat Diet (HFD) and low dose of streptozotocin (STZ) at 30, 35, and 40 mg/kg was used to induce metabolic syndrome in the setting of diabetes mellitus in Wistar rats. Results. The 40 mg/kg STZ produced sustained hyperglycemia and the dose was thus selected for the study to induce diabetes mellitus. Various components of metabolic syndrome such as dyslipidemia {(increased triglyceride, total cholesterol, LDL cholesterol, and decreased HDL cholesterol)}, diabetes mellitus (blood glucose, HbA1c, serum insulin, and C-peptide), and hypertension {systolic blood pressure} were mimicked in the developed model of metabolic syndrome co-existing with diabetes mellitus. In addition to significant cardiac injury, atherogenic index, inflammation (hs-CRP), decline in hepatic and renal function were observed in the HF-DC group when compared to NC group rats. The histopathological assessment confirmed presence of edema, necrosis, and inflammation in heart, pancreas, liver, and kidney of HF-DC group as compared to NC. Conclusion. The present study has developed a unique rodent model of metabolic syndrome, with diabetes as an essential component. Hindawi Publishing Corporation 2016 2016-01-06 /pmc/articles/PMC4736803/ /pubmed/26880906 http://dx.doi.org/10.1155/2016/9463476 Text en Copyright © 2016 Rajesh Kumar Suman et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Suman, Rajesh Kumar
Ray Mohanty, Ipseeta
Borde, Manjusha K.
Maheshwari, Ujwala
Deshmukh, Y. A.
Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats
title Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats
title_full Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats
title_fullStr Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats
title_full_unstemmed Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats
title_short Development of an Experimental Model of Diabetes Co-Existing with Metabolic Syndrome in Rats
title_sort development of an experimental model of diabetes co-existing with metabolic syndrome in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736803/
https://www.ncbi.nlm.nih.gov/pubmed/26880906
http://dx.doi.org/10.1155/2016/9463476
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