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Subattomole detection of adiponectin in urine by ultrasensitive ELISA coupled with thio-NAD cycling
Adiponectin is a hormone secreted from adipocytes, and it demonstrates antidiabetic, anti-atherosclerotic, antiobesity and anti-inflammatory effects. However, the patterns of change in urinary adiponectin levels in various diseases remain unknown, because only trace amounts of the hormone are presen...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Biophysical Society of Japan (BSJ)
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736831/ https://www.ncbi.nlm.nih.gov/pubmed/27493857 http://dx.doi.org/10.2142/biophysico.12.0_79 |
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author | Morikawa, Mika Naito, Rina Mita, Koichi Watabe, Satoshi Nakaishi, Kazunari Yoshimura, Teruki Miura, Toshiaki Hashida, Seiichi Ito, Etsuro |
author_facet | Morikawa, Mika Naito, Rina Mita, Koichi Watabe, Satoshi Nakaishi, Kazunari Yoshimura, Teruki Miura, Toshiaki Hashida, Seiichi Ito, Etsuro |
author_sort | Morikawa, Mika |
collection | PubMed |
description | Adiponectin is a hormone secreted from adipocytes, and it demonstrates antidiabetic, anti-atherosclerotic, antiobesity and anti-inflammatory effects. However, the patterns of change in urinary adiponectin levels in various diseases remain unknown, because only trace amounts of the hormone are present in urine. In the present study, we applied an ultrasensitive ELISA coupled with thio-NAD cycling to measure urinary adiponectin levels. Spikeand-recovery tests using urine confirmed the reliability of our ultrasensitive ELISA. The limit of detection for adiponectin in urine was 2.3×10(−19) moles/assay (1.4 pg/mL). The urinary adiponectin concentration ranged between 0.04 and 5.82 ng/mL in healthy subjects. The pilot study showed that the urinary adiponectin levels, which were corrected by the creatinine concentration, were 0.73±0.50 (ng/mg creatinine, N=6) for healthy subjects, versus 12.02±3.85 (ng/mg creatinine, N=3) for patients with diabetes mellitus (DM). That is, the urinary adiponectin levels were higher (P<0.05) in DM patients than in healthy subjects. Further, these urinary adiponectin levels tended to increase with the progression of DM accompanied with nephropathy. Our method is thus expected to provide a simple, rapid and reasonably priced test for noninvasive monitoring of the progression of DM without the requirement of special tools. |
format | Online Article Text |
id | pubmed-4736831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Biophysical Society of Japan (BSJ) |
record_format | MEDLINE/PubMed |
spelling | pubmed-47368312016-08-04 Subattomole detection of adiponectin in urine by ultrasensitive ELISA coupled with thio-NAD cycling Morikawa, Mika Naito, Rina Mita, Koichi Watabe, Satoshi Nakaishi, Kazunari Yoshimura, Teruki Miura, Toshiaki Hashida, Seiichi Ito, Etsuro Biophys Physicobiol Regular Article Adiponectin is a hormone secreted from adipocytes, and it demonstrates antidiabetic, anti-atherosclerotic, antiobesity and anti-inflammatory effects. However, the patterns of change in urinary adiponectin levels in various diseases remain unknown, because only trace amounts of the hormone are present in urine. In the present study, we applied an ultrasensitive ELISA coupled with thio-NAD cycling to measure urinary adiponectin levels. Spikeand-recovery tests using urine confirmed the reliability of our ultrasensitive ELISA. The limit of detection for adiponectin in urine was 2.3×10(−19) moles/assay (1.4 pg/mL). The urinary adiponectin concentration ranged between 0.04 and 5.82 ng/mL in healthy subjects. The pilot study showed that the urinary adiponectin levels, which were corrected by the creatinine concentration, were 0.73±0.50 (ng/mg creatinine, N=6) for healthy subjects, versus 12.02±3.85 (ng/mg creatinine, N=3) for patients with diabetes mellitus (DM). That is, the urinary adiponectin levels were higher (P<0.05) in DM patients than in healthy subjects. Further, these urinary adiponectin levels tended to increase with the progression of DM accompanied with nephropathy. Our method is thus expected to provide a simple, rapid and reasonably priced test for noninvasive monitoring of the progression of DM without the requirement of special tools. The Biophysical Society of Japan (BSJ) 2015-11-12 /pmc/articles/PMC4736831/ /pubmed/27493857 http://dx.doi.org/10.2142/biophysico.12.0_79 Text en 2015 © The Biophysical Society of Japan This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Article Morikawa, Mika Naito, Rina Mita, Koichi Watabe, Satoshi Nakaishi, Kazunari Yoshimura, Teruki Miura, Toshiaki Hashida, Seiichi Ito, Etsuro Subattomole detection of adiponectin in urine by ultrasensitive ELISA coupled with thio-NAD cycling |
title | Subattomole detection of adiponectin in urine by ultrasensitive ELISA coupled with thio-NAD cycling |
title_full | Subattomole detection of adiponectin in urine by ultrasensitive ELISA coupled with thio-NAD cycling |
title_fullStr | Subattomole detection of adiponectin in urine by ultrasensitive ELISA coupled with thio-NAD cycling |
title_full_unstemmed | Subattomole detection of adiponectin in urine by ultrasensitive ELISA coupled with thio-NAD cycling |
title_short | Subattomole detection of adiponectin in urine by ultrasensitive ELISA coupled with thio-NAD cycling |
title_sort | subattomole detection of adiponectin in urine by ultrasensitive elisa coupled with thio-nad cycling |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736831/ https://www.ncbi.nlm.nih.gov/pubmed/27493857 http://dx.doi.org/10.2142/biophysico.12.0_79 |
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