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Neuroprotective Effect of Compound Anisodine in a Mouse Model with Chronic Ocular Hypertension
BACKGROUND: Compound anisodine (CA) is a compound preparation made from hydrobromide anisodine and procaine hydrochloride. The former is an M-choline receptor blocker with the function of regulating the vegetative nervous system, improving microcirculation, and so on. The latter is an antioxidant wi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736849/ https://www.ncbi.nlm.nih.gov/pubmed/26415805 http://dx.doi.org/10.4103/0366-6999.166043 |
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author | Liu, Wen-Dong Chen, Lan-Lan Shen, Ce-Ying Jiang, Li-Bin |
author_facet | Liu, Wen-Dong Chen, Lan-Lan Shen, Ce-Ying Jiang, Li-Bin |
author_sort | Liu, Wen-Dong |
collection | PubMed |
description | BACKGROUND: Compound anisodine (CA) is a compound preparation made from hydrobromide anisodine and procaine hydrochloride. The former is an M-choline receptor blocker with the function of regulating the vegetative nervous system, improving microcirculation, and so on. The latter is an antioxidant with the activities of neuroprotection. This study aimed to investigate the potential neuroprotection of CA, which affects the degeneration of the retinal ganglion cells (RGCs) in an animal model with chronic ocular hypertension. METHODS: Female C57BL/6J mice (n = 24) were divided randomly into four groups: Normal control group without any treatment (Group A, n = 6); CA control group with feeding the CA solution (Group B, n = 6); microbeads (MBs) control group with injecting MB into the anterior chamber (Group C, n = 6); CA study group with MB injection and with feeding the CA solution (Group D, n = 6). Intraocular pressure (IOP) was measured every 3 days after MB injection. At the 21(st) day, neurons were retrograde-labeled by Fluoro-Gold (FG). Animals were sacrificed on the 27(th) day. Retinal flat mounts were stained immunohistologically by β-III-tubulin. FG-retrograde-labeled RGCs, β-III-tubulin-positive RGCs, and β-III-tubulin-positive nerve fibers were quantified. RESULTS: Mice of Groups C and D expressed the incidence of consistent IOP elevation, which is above the IOP level of Group A with the normal one. There is no significant difference in IOP between Groups A and B (P > 0.05). On the 27(th) day, there were distinct loss in stained RGCs and nerve fibers from Groups C and D compared with Group A (all P < 0.001). The quantity was significantly higher in Group D as compared to Group C (all P < 0.001) but lower than Group A (all P < 0.001). There was no significant difference in the quantity of RGCs and nerve fibers between Groups A and B (all P > 0.05). CONCLUSIONS: These findings suggest that CA plays an importantly neuroprotective role on RGCs in a mouse model with chronic ocular hypertension. |
format | Online Article Text |
id | pubmed-4736849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-47368492016-04-04 Neuroprotective Effect of Compound Anisodine in a Mouse Model with Chronic Ocular Hypertension Liu, Wen-Dong Chen, Lan-Lan Shen, Ce-Ying Jiang, Li-Bin Chin Med J (Engl) Original Article BACKGROUND: Compound anisodine (CA) is a compound preparation made from hydrobromide anisodine and procaine hydrochloride. The former is an M-choline receptor blocker with the function of regulating the vegetative nervous system, improving microcirculation, and so on. The latter is an antioxidant with the activities of neuroprotection. This study aimed to investigate the potential neuroprotection of CA, which affects the degeneration of the retinal ganglion cells (RGCs) in an animal model with chronic ocular hypertension. METHODS: Female C57BL/6J mice (n = 24) were divided randomly into four groups: Normal control group without any treatment (Group A, n = 6); CA control group with feeding the CA solution (Group B, n = 6); microbeads (MBs) control group with injecting MB into the anterior chamber (Group C, n = 6); CA study group with MB injection and with feeding the CA solution (Group D, n = 6). Intraocular pressure (IOP) was measured every 3 days after MB injection. At the 21(st) day, neurons were retrograde-labeled by Fluoro-Gold (FG). Animals were sacrificed on the 27(th) day. Retinal flat mounts were stained immunohistologically by β-III-tubulin. FG-retrograde-labeled RGCs, β-III-tubulin-positive RGCs, and β-III-tubulin-positive nerve fibers were quantified. RESULTS: Mice of Groups C and D expressed the incidence of consistent IOP elevation, which is above the IOP level of Group A with the normal one. There is no significant difference in IOP between Groups A and B (P > 0.05). On the 27(th) day, there were distinct loss in stained RGCs and nerve fibers from Groups C and D compared with Group A (all P < 0.001). The quantity was significantly higher in Group D as compared to Group C (all P < 0.001) but lower than Group A (all P < 0.001). There was no significant difference in the quantity of RGCs and nerve fibers between Groups A and B (all P > 0.05). CONCLUSIONS: These findings suggest that CA plays an importantly neuroprotective role on RGCs in a mouse model with chronic ocular hypertension. Medknow Publications & Media Pvt Ltd 2015-10-05 /pmc/articles/PMC4736849/ /pubmed/26415805 http://dx.doi.org/10.4103/0366-6999.166043 Text en Copyright: © 2015 Chinese Medical Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Liu, Wen-Dong Chen, Lan-Lan Shen, Ce-Ying Jiang, Li-Bin Neuroprotective Effect of Compound Anisodine in a Mouse Model with Chronic Ocular Hypertension |
title | Neuroprotective Effect of Compound Anisodine in a Mouse Model with Chronic Ocular Hypertension |
title_full | Neuroprotective Effect of Compound Anisodine in a Mouse Model with Chronic Ocular Hypertension |
title_fullStr | Neuroprotective Effect of Compound Anisodine in a Mouse Model with Chronic Ocular Hypertension |
title_full_unstemmed | Neuroprotective Effect of Compound Anisodine in a Mouse Model with Chronic Ocular Hypertension |
title_short | Neuroprotective Effect of Compound Anisodine in a Mouse Model with Chronic Ocular Hypertension |
title_sort | neuroprotective effect of compound anisodine in a mouse model with chronic ocular hypertension |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736849/ https://www.ncbi.nlm.nih.gov/pubmed/26415805 http://dx.doi.org/10.4103/0366-6999.166043 |
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