Cargando…

The expression of mouse CLEC‐2 on leucocyte subsets varies according to their anatomical location and inflammatory state

Expression of mouse C‐type lectin‐like receptor 2 (CLEC‐2) has been reported on circulating CD11b(high) Gr‐1(high) myeloid cells and dendritic cells (DCs) under basal conditions, as well as on a variety of leucocyte subsets following inflammatory stimuli or in vitro cell culture. However, previous s...

Descripción completa

Detalles Bibliográficos
Autores principales: Lowe, Kate L., Navarro‐Núñez, Leyre, Bénézech, Cécile, Nayar, Saba, Kingston, Bethany L., Nieswandt, Bernhard, Barone, Francesca, Watson, Steve P., Buckley, Christopher D., Desanti, Guillaume E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737233/
https://www.ncbi.nlm.nih.gov/pubmed/26173808
http://dx.doi.org/10.1002/eji.201445314
_version_ 1782413445389352960
author Lowe, Kate L.
Navarro‐Núñez, Leyre
Bénézech, Cécile
Nayar, Saba
Kingston, Bethany L.
Nieswandt, Bernhard
Barone, Francesca
Watson, Steve P.
Buckley, Christopher D.
Desanti, Guillaume E.
author_facet Lowe, Kate L.
Navarro‐Núñez, Leyre
Bénézech, Cécile
Nayar, Saba
Kingston, Bethany L.
Nieswandt, Bernhard
Barone, Francesca
Watson, Steve P.
Buckley, Christopher D.
Desanti, Guillaume E.
author_sort Lowe, Kate L.
collection PubMed
description Expression of mouse C‐type lectin‐like receptor 2 (CLEC‐2) has been reported on circulating CD11b(high) Gr‐1(high) myeloid cells and dendritic cells (DCs) under basal conditions, as well as on a variety of leucocyte subsets following inflammatory stimuli or in vitro cell culture. However, previous studies assessing CLEC‐2 expression failed to use CLEC‐2‐deficient mice as negative controls and instead relied heavily on single antibody clones. Here, we generated CLEC‐2‐deficient adult mice using two independent approaches and employed two anti‐mouse CLEC‐2 antibody clones to investigate surface expression on hematopoietic cells from peripheral blood and secondary lymphoid organs. We rule out constitutive CLEC‐2 expression on resting DCs and show that CLEC‐2 is upregulated in response to LPS‐induced systemic inflammation in a small subset of activated DCs isolated from the mesenteric lymph nodes but not the spleen. Moreover, we demonstrate for the first time that peripheral blood B lymphocytes present exogenously derived CLEC‐2 and suggest that both circulating B lymphocytes and CD11b(high) Gr‐1(high) myeloid cells lose CLEC‐2 following entry into secondary lymphoid organs. These results have significant implications for our understanding of CLEC‐2 physiological functions
format Online
Article
Text
id pubmed-4737233
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-47372332016-02-11 The expression of mouse CLEC‐2 on leucocyte subsets varies according to their anatomical location and inflammatory state Lowe, Kate L. Navarro‐Núñez, Leyre Bénézech, Cécile Nayar, Saba Kingston, Bethany L. Nieswandt, Bernhard Barone, Francesca Watson, Steve P. Buckley, Christopher D. Desanti, Guillaume E. Eur J Immunol Cellular Immune Response Expression of mouse C‐type lectin‐like receptor 2 (CLEC‐2) has been reported on circulating CD11b(high) Gr‐1(high) myeloid cells and dendritic cells (DCs) under basal conditions, as well as on a variety of leucocyte subsets following inflammatory stimuli or in vitro cell culture. However, previous studies assessing CLEC‐2 expression failed to use CLEC‐2‐deficient mice as negative controls and instead relied heavily on single antibody clones. Here, we generated CLEC‐2‐deficient adult mice using two independent approaches and employed two anti‐mouse CLEC‐2 antibody clones to investigate surface expression on hematopoietic cells from peripheral blood and secondary lymphoid organs. We rule out constitutive CLEC‐2 expression on resting DCs and show that CLEC‐2 is upregulated in response to LPS‐induced systemic inflammation in a small subset of activated DCs isolated from the mesenteric lymph nodes but not the spleen. Moreover, we demonstrate for the first time that peripheral blood B lymphocytes present exogenously derived CLEC‐2 and suggest that both circulating B lymphocytes and CD11b(high) Gr‐1(high) myeloid cells lose CLEC‐2 following entry into secondary lymphoid organs. These results have significant implications for our understanding of CLEC‐2 physiological functions John Wiley and Sons Inc. 2015-08-12 2015-09 /pmc/articles/PMC4737233/ /pubmed/26173808 http://dx.doi.org/10.1002/eji.201445314 Text en © 2015 The Authors. European Journal of Immunology published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cellular Immune Response
Lowe, Kate L.
Navarro‐Núñez, Leyre
Bénézech, Cécile
Nayar, Saba
Kingston, Bethany L.
Nieswandt, Bernhard
Barone, Francesca
Watson, Steve P.
Buckley, Christopher D.
Desanti, Guillaume E.
The expression of mouse CLEC‐2 on leucocyte subsets varies according to their anatomical location and inflammatory state
title The expression of mouse CLEC‐2 on leucocyte subsets varies according to their anatomical location and inflammatory state
title_full The expression of mouse CLEC‐2 on leucocyte subsets varies according to their anatomical location and inflammatory state
title_fullStr The expression of mouse CLEC‐2 on leucocyte subsets varies according to their anatomical location and inflammatory state
title_full_unstemmed The expression of mouse CLEC‐2 on leucocyte subsets varies according to their anatomical location and inflammatory state
title_short The expression of mouse CLEC‐2 on leucocyte subsets varies according to their anatomical location and inflammatory state
title_sort expression of mouse clec‐2 on leucocyte subsets varies according to their anatomical location and inflammatory state
topic Cellular Immune Response
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737233/
https://www.ncbi.nlm.nih.gov/pubmed/26173808
http://dx.doi.org/10.1002/eji.201445314
work_keys_str_mv AT lowekatel theexpressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT navarronunezleyre theexpressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT benezechcecile theexpressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT nayarsaba theexpressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT kingstonbethanyl theexpressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT nieswandtbernhard theexpressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT baronefrancesca theexpressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT watsonstevep theexpressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT buckleychristopherd theexpressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT desantiguillaumee theexpressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT lowekatel expressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT navarronunezleyre expressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT benezechcecile expressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT nayarsaba expressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT kingstonbethanyl expressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT nieswandtbernhard expressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT baronefrancesca expressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT watsonstevep expressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT buckleychristopherd expressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate
AT desantiguillaumee expressionofmouseclec2onleucocytesubsetsvariesaccordingtotheiranatomicallocationandinflammatorystate