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A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue

Fluorescence lifetime imaging (FLIM) has previously been shown to provide contrast between normal and diseased tissue. Here we present progress towards clinical and preclinical FLIM endoscopy of tissue autofluorescence, demonstrating a flexible wide‐field endoscope that utilised a low average power...

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Autores principales: Sparks, Hugh, Warren, Sean, Guedes, Joana, Yoshida, Nagisa, Charn, Tze Choong, Guerra, Nadia, Tatla, Taranjit, Dunsby, Christopher, French, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY‐VCH Verlag 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737404/
https://www.ncbi.nlm.nih.gov/pubmed/24573953
http://dx.doi.org/10.1002/jbio.201300203
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author Sparks, Hugh
Warren, Sean
Guedes, Joana
Yoshida, Nagisa
Charn, Tze Choong
Guerra, Nadia
Tatla, Taranjit
Dunsby, Christopher
French, Paul
author_facet Sparks, Hugh
Warren, Sean
Guedes, Joana
Yoshida, Nagisa
Charn, Tze Choong
Guerra, Nadia
Tatla, Taranjit
Dunsby, Christopher
French, Paul
author_sort Sparks, Hugh
collection PubMed
description Fluorescence lifetime imaging (FLIM) has previously been shown to provide contrast between normal and diseased tissue. Here we present progress towards clinical and preclinical FLIM endoscopy of tissue autofluorescence, demonstrating a flexible wide‐field endoscope that utilised a low average power blue picosecond laser diode excitation source and was able to acquire ∼mm‐scale spatial maps of autofluorescence lifetimes from fresh ex vivo diseased human larynx biopsies in ∼8 seconds using an average excitation power of ∼0.5 mW at the specimen. To illustrate its potential for FLIM at higher acquisition rates, a higher power mode‐locked frequency doubled Ti:Sapphire laser was used to demonstrate FLIM of ex vivo mouse bowel at up to 2.5 Hz using 10 mW of average excitation power at the specimen. (© 2014 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim)
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spelling pubmed-47374042016-02-12 A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue Sparks, Hugh Warren, Sean Guedes, Joana Yoshida, Nagisa Charn, Tze Choong Guerra, Nadia Tatla, Taranjit Dunsby, Christopher French, Paul J Biophotonics Editor's Choice Fluorescence lifetime imaging (FLIM) has previously been shown to provide contrast between normal and diseased tissue. Here we present progress towards clinical and preclinical FLIM endoscopy of tissue autofluorescence, demonstrating a flexible wide‐field endoscope that utilised a low average power blue picosecond laser diode excitation source and was able to acquire ∼mm‐scale spatial maps of autofluorescence lifetimes from fresh ex vivo diseased human larynx biopsies in ∼8 seconds using an average excitation power of ∼0.5 mW at the specimen. To illustrate its potential for FLIM at higher acquisition rates, a higher power mode‐locked frequency doubled Ti:Sapphire laser was used to demonstrate FLIM of ex vivo mouse bowel at up to 2.5 Hz using 10 mW of average excitation power at the specimen. (© 2014 WILEY‐VCH Verlag GmbH &Co. KGaA, Weinheim) WILEY‐VCH Verlag 2014-02-27 2015-01 /pmc/articles/PMC4737404/ /pubmed/24573953 http://dx.doi.org/10.1002/jbio.201300203 Text en © 2015 The Authors. Journal of Biophotonics published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Editor's Choice
Sparks, Hugh
Warren, Sean
Guedes, Joana
Yoshida, Nagisa
Charn, Tze Choong
Guerra, Nadia
Tatla, Taranjit
Dunsby, Christopher
French, Paul
A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue
title A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue
title_full A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue
title_fullStr A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue
title_full_unstemmed A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue
title_short A flexible wide‐field FLIM endoscope utilising blue excitation light for label‐free contrast of tissue
title_sort flexible wide‐field flim endoscope utilising blue excitation light for label‐free contrast of tissue
topic Editor's Choice
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737404/
https://www.ncbi.nlm.nih.gov/pubmed/24573953
http://dx.doi.org/10.1002/jbio.201300203
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