Serum apolipoprotein A-1 quantification by LC–MS with a SILAC internal standard reveals reduced levels in smokers

BACKGROUND: Absolute quantification of protein biomarkers such as serum apolipoprotein A1 by both immunoassays and LC–MS can provide misleading results. RESULTS: Recombinant ApoA-1 internal standard was prepared using stable isotope labeling by amino acids in cell culture with [(13)C(6)(15)N(2)]-lys...

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Detalles Bibliográficos
Autores principales: Wang, Qingqing, Zhang, Suhong, Guo, Lili, Busch, Christine M, Jian, Wenying, Weng, Naidong, Snyder, Nathaniel W, Rangiah, Kannan, Mesaros, Clementina, Blair, Ian A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737526/
https://www.ncbi.nlm.nih.gov/pubmed/26394123
http://dx.doi.org/10.4155/bio.15.195
Descripción
Sumario:BACKGROUND: Absolute quantification of protein biomarkers such as serum apolipoprotein A1 by both immunoassays and LC–MS can provide misleading results. RESULTS: Recombinant ApoA-1 internal standard was prepared using stable isotope labeling by amino acids in cell culture with [(13)C(6)(15)N(2)]-lysine and [(13)C(9)(15)N(1)]-tyrosine in human cells. A stable isotope dilution LC–MS method for serum ApoA-1 was validated and levels analyzed for 50 nonsmokers and 50 smokers. CONCLUSION: The concentration of ApoA-1 in nonsmokers was 169.4 mg/dl with an 18.4% reduction to 138.2 mg/dl in smokers. The validated assay will have clinical utility for assessing effects of smoking cessation and therapeutic or dietary interventions in high-risk populations.

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