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Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation
The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed 'Opto-...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737651/ https://www.ncbi.nlm.nih.gov/pubmed/26646180 http://dx.doi.org/10.7554/eLife.10024 |
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author | He, Lian Zhang, Yuanwei Ma, Guolin Tan, Peng Li, Zhanjun Zang, Shengbing Wu, Xiang Jing, Ji Fang, Shaohai Zhou, Lijuan Wang, Youjun Huang, Yun Hogan, Patrick G Han, Gang Zhou, Yubin |
author_facet | He, Lian Zhang, Yuanwei Ma, Guolin Tan, Peng Li, Zhanjun Zang, Shengbing Wu, Xiang Jing, Ji Fang, Shaohai Zhou, Lijuan Wang, Youjun Huang, Yun Hogan, Patrick G Han, Gang Zhou, Yubin |
author_sort | He, Lian |
collection | PubMed |
description | The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed 'Opto-CRAC') that selectively and remotely controls Ca(2+) oscillations and Ca(2+)-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca(2+)-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded 'photoactivatable adjuvant' to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote and wireless control of Ca(2+)-modulated activities with tailored function. DOI: http://dx.doi.org/10.7554/eLife.10024.001 |
format | Online Article Text |
id | pubmed-4737651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-47376512016-02-04 Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation He, Lian Zhang, Yuanwei Ma, Guolin Tan, Peng Li, Zhanjun Zang, Shengbing Wu, Xiang Jing, Ji Fang, Shaohai Zhou, Lijuan Wang, Youjun Huang, Yun Hogan, Patrick G Han, Gang Zhou, Yubin eLife Biochemistry The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed 'Opto-CRAC') that selectively and remotely controls Ca(2+) oscillations and Ca(2+)-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca(2+)-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded 'photoactivatable adjuvant' to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote and wireless control of Ca(2+)-modulated activities with tailored function. DOI: http://dx.doi.org/10.7554/eLife.10024.001 eLife Sciences Publications, Ltd 2015-12-08 /pmc/articles/PMC4737651/ /pubmed/26646180 http://dx.doi.org/10.7554/eLife.10024 Text en © 2015, He et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry He, Lian Zhang, Yuanwei Ma, Guolin Tan, Peng Li, Zhanjun Zang, Shengbing Wu, Xiang Jing, Ji Fang, Shaohai Zhou, Lijuan Wang, Youjun Huang, Yun Hogan, Patrick G Han, Gang Zhou, Yubin Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation |
title | Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation |
title_full | Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation |
title_fullStr | Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation |
title_full_unstemmed | Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation |
title_short | Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation |
title_sort | near-infrared photoactivatable control of ca(2+) signaling and optogenetic immunomodulation |
topic | Biochemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737651/ https://www.ncbi.nlm.nih.gov/pubmed/26646180 http://dx.doi.org/10.7554/eLife.10024 |
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