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Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis
AREB (ABA response element binding) proteins in plants play direct regulatory roles in response to multiple stresses, but their functions in wheat (Triticum aestivum L.) are not clear. In the present study, TaAREB3, a new member of the AREB transcription factor family, was isolated from wheat. Seque...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737681/ https://www.ncbi.nlm.nih.gov/pubmed/26884722 http://dx.doi.org/10.7150/ijbs.13538 |
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author | Wang, Jingyi Li, Qian Mao, Xinguo Li, Ang Jing, Ruilian |
author_facet | Wang, Jingyi Li, Qian Mao, Xinguo Li, Ang Jing, Ruilian |
author_sort | Wang, Jingyi |
collection | PubMed |
description | AREB (ABA response element binding) proteins in plants play direct regulatory roles in response to multiple stresses, but their functions in wheat (Triticum aestivum L.) are not clear. In the present study, TaAREB3, a new member of the AREB transcription factor family, was isolated from wheat. Sequence analysis showed that the TaAREB3 protein is composed of three parts, a conserved N-terminal, a variable M region, and a conserved C-terminal with a bZIP domain. It belongs to the group A subfamily of bZIP transcription factors. TaAREB3 was constitutively expressed in stems, leaves, florets, anthers, pistils, seeds, and most highly, in roots. TaAREB3 gene expression was induced with abscisic acid (ABA) and low temperature stress, and its protein was localized in the nucleus when transiently expressed in tobacco epidermal cells and stably expressed in transgenic Arabidopsis. TaAREB3 protein has transcriptional activation activity, and can bind to the ABRE cis-element in vitro. Overexpression of TaAREB3 in Arabidopsis not only enhanced ABA sensitivity, but also strengthened drought and freezing tolerances. TaAREB3 also activated RD29A, RD29B, COR15A, and COR47 by binding to their promoter regions in transgenic Arabidopsis. These results demonstrated that TaAREB3 plays an important role in drought and freezing tolerances in Arabidopsis. |
format | Online Article Text |
id | pubmed-4737681 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-47376812016-02-16 Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis Wang, Jingyi Li, Qian Mao, Xinguo Li, Ang Jing, Ruilian Int J Biol Sci Research Paper AREB (ABA response element binding) proteins in plants play direct regulatory roles in response to multiple stresses, but their functions in wheat (Triticum aestivum L.) are not clear. In the present study, TaAREB3, a new member of the AREB transcription factor family, was isolated from wheat. Sequence analysis showed that the TaAREB3 protein is composed of three parts, a conserved N-terminal, a variable M region, and a conserved C-terminal with a bZIP domain. It belongs to the group A subfamily of bZIP transcription factors. TaAREB3 was constitutively expressed in stems, leaves, florets, anthers, pistils, seeds, and most highly, in roots. TaAREB3 gene expression was induced with abscisic acid (ABA) and low temperature stress, and its protein was localized in the nucleus when transiently expressed in tobacco epidermal cells and stably expressed in transgenic Arabidopsis. TaAREB3 protein has transcriptional activation activity, and can bind to the ABRE cis-element in vitro. Overexpression of TaAREB3 in Arabidopsis not only enhanced ABA sensitivity, but also strengthened drought and freezing tolerances. TaAREB3 also activated RD29A, RD29B, COR15A, and COR47 by binding to their promoter regions in transgenic Arabidopsis. These results demonstrated that TaAREB3 plays an important role in drought and freezing tolerances in Arabidopsis. Ivyspring International Publisher 2016-01-01 /pmc/articles/PMC4737681/ /pubmed/26884722 http://dx.doi.org/10.7150/ijbs.13538 Text en © Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. |
spellingShingle | Research Paper Wang, Jingyi Li, Qian Mao, Xinguo Li, Ang Jing, Ruilian Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis |
title | Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis |
title_full | Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis |
title_fullStr | Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis |
title_full_unstemmed | Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis |
title_short | Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis |
title_sort | wheat transcription factor taareb3 participates in drought and freezing tolerances in arabidopsis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737681/ https://www.ncbi.nlm.nih.gov/pubmed/26884722 http://dx.doi.org/10.7150/ijbs.13538 |
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