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Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis

AREB (ABA response element binding) proteins in plants play direct regulatory roles in response to multiple stresses, but their functions in wheat (Triticum aestivum L.) are not clear. In the present study, TaAREB3, a new member of the AREB transcription factor family, was isolated from wheat. Seque...

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Autores principales: Wang, Jingyi, Li, Qian, Mao, Xinguo, Li, Ang, Jing, Ruilian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737681/
https://www.ncbi.nlm.nih.gov/pubmed/26884722
http://dx.doi.org/10.7150/ijbs.13538
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author Wang, Jingyi
Li, Qian
Mao, Xinguo
Li, Ang
Jing, Ruilian
author_facet Wang, Jingyi
Li, Qian
Mao, Xinguo
Li, Ang
Jing, Ruilian
author_sort Wang, Jingyi
collection PubMed
description AREB (ABA response element binding) proteins in plants play direct regulatory roles in response to multiple stresses, but their functions in wheat (Triticum aestivum L.) are not clear. In the present study, TaAREB3, a new member of the AREB transcription factor family, was isolated from wheat. Sequence analysis showed that the TaAREB3 protein is composed of three parts, a conserved N-terminal, a variable M region, and a conserved C-terminal with a bZIP domain. It belongs to the group A subfamily of bZIP transcription factors. TaAREB3 was constitutively expressed in stems, leaves, florets, anthers, pistils, seeds, and most highly, in roots. TaAREB3 gene expression was induced with abscisic acid (ABA) and low temperature stress, and its protein was localized in the nucleus when transiently expressed in tobacco epidermal cells and stably expressed in transgenic Arabidopsis. TaAREB3 protein has transcriptional activation activity, and can bind to the ABRE cis-element in vitro. Overexpression of TaAREB3 in Arabidopsis not only enhanced ABA sensitivity, but also strengthened drought and freezing tolerances. TaAREB3 also activated RD29A, RD29B, COR15A, and COR47 by binding to their promoter regions in transgenic Arabidopsis. These results demonstrated that TaAREB3 plays an important role in drought and freezing tolerances in Arabidopsis.
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spelling pubmed-47376812016-02-16 Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis Wang, Jingyi Li, Qian Mao, Xinguo Li, Ang Jing, Ruilian Int J Biol Sci Research Paper AREB (ABA response element binding) proteins in plants play direct regulatory roles in response to multiple stresses, but their functions in wheat (Triticum aestivum L.) are not clear. In the present study, TaAREB3, a new member of the AREB transcription factor family, was isolated from wheat. Sequence analysis showed that the TaAREB3 protein is composed of three parts, a conserved N-terminal, a variable M region, and a conserved C-terminal with a bZIP domain. It belongs to the group A subfamily of bZIP transcription factors. TaAREB3 was constitutively expressed in stems, leaves, florets, anthers, pistils, seeds, and most highly, in roots. TaAREB3 gene expression was induced with abscisic acid (ABA) and low temperature stress, and its protein was localized in the nucleus when transiently expressed in tobacco epidermal cells and stably expressed in transgenic Arabidopsis. TaAREB3 protein has transcriptional activation activity, and can bind to the ABRE cis-element in vitro. Overexpression of TaAREB3 in Arabidopsis not only enhanced ABA sensitivity, but also strengthened drought and freezing tolerances. TaAREB3 also activated RD29A, RD29B, COR15A, and COR47 by binding to their promoter regions in transgenic Arabidopsis. These results demonstrated that TaAREB3 plays an important role in drought and freezing tolerances in Arabidopsis. Ivyspring International Publisher 2016-01-01 /pmc/articles/PMC4737681/ /pubmed/26884722 http://dx.doi.org/10.7150/ijbs.13538 Text en © Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions.
spellingShingle Research Paper
Wang, Jingyi
Li, Qian
Mao, Xinguo
Li, Ang
Jing, Ruilian
Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis
title Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis
title_full Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis
title_fullStr Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis
title_full_unstemmed Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis
title_short Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis
title_sort wheat transcription factor taareb3 participates in drought and freezing tolerances in arabidopsis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737681/
https://www.ncbi.nlm.nih.gov/pubmed/26884722
http://dx.doi.org/10.7150/ijbs.13538
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