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Crosstalk between adipose-derived stem cells and chondrocytes: when growth factors matter

Adipose-derived stem cells (ASCs) and mesenchymal stem cells are promising for tissue repair because of their multilineage differentiation capacity. Our previous data confirmed that the implantation of mixed ASCs and chondrocytes into cartilage defects induced desirable in vivo healing outcomes. How...

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Autores principales: Zhong, Juan, Guo, Bin, Xie, Jing, Deng, Shuwen, Fu, Na, Lin, Shiyu, Li, Guo, Lin, Yunfeng, Cai, Xiaoxiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738199/
https://www.ncbi.nlm.nih.gov/pubmed/26848404
http://dx.doi.org/10.1038/boneres.2015.36
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author Zhong, Juan
Guo, Bin
Xie, Jing
Deng, Shuwen
Fu, Na
Lin, Shiyu
Li, Guo
Lin, Yunfeng
Cai, Xiaoxiao
author_facet Zhong, Juan
Guo, Bin
Xie, Jing
Deng, Shuwen
Fu, Na
Lin, Shiyu
Li, Guo
Lin, Yunfeng
Cai, Xiaoxiao
author_sort Zhong, Juan
collection PubMed
description Adipose-derived stem cells (ASCs) and mesenchymal stem cells are promising for tissue repair because of their multilineage differentiation capacity. Our previous data confirmed that the implantation of mixed ASCs and chondrocytes into cartilage defects induced desirable in vivo healing outcomes. However, the paracrine action of ASCs on chondrocytes needs to be further elucidated. In this study, we established a co-culture system to achieve cell-to-cell and cell-to-tissue crosstalk and explored the soluble growth factors in both ASCs and chondrocytes supplemented with 1% fetal bovine serum to mimic the physiological microenvironment. In ASCs, we screened for growth factors by semi-quantitative PCR and quantitative real-time PCR and found that the expression of bone morphogenetic protein 2 (BMP-2), vascular endothelial growth factor B (VEGFB), hypoxia inducible factor-1α (HIF-1α), fibroblast growth factor-2 (FGF-2), and transforming growth factor-β1 significantly increased after co-culture in comparison with mono-culture. In chondrocytes, VEGFA was significantly enhanced after co-culture. Unexpectedly, the expression of collagen II and aggrecan was significantly down-regulated in the co-culture group compared with the mono-culture group. Meanwhile, among all the growth factors screened, we found that the BMP family members BMP-2, BMP-4, and BMP-5 were down-regulated and that VEGFB, HIF-1α, FGF-2, and PDGF were significantly decreased after co-culture. These results suggest that crosstalk between ASCs and chondrocytes is a pathway through the regulated growth factors that might have potential in cartilage repair and regeneration and could be useful for tissue engineering.
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spelling pubmed-47381992016-02-04 Crosstalk between adipose-derived stem cells and chondrocytes: when growth factors matter Zhong, Juan Guo, Bin Xie, Jing Deng, Shuwen Fu, Na Lin, Shiyu Li, Guo Lin, Yunfeng Cai, Xiaoxiao Bone Res Article Adipose-derived stem cells (ASCs) and mesenchymal stem cells are promising for tissue repair because of their multilineage differentiation capacity. Our previous data confirmed that the implantation of mixed ASCs and chondrocytes into cartilage defects induced desirable in vivo healing outcomes. However, the paracrine action of ASCs on chondrocytes needs to be further elucidated. In this study, we established a co-culture system to achieve cell-to-cell and cell-to-tissue crosstalk and explored the soluble growth factors in both ASCs and chondrocytes supplemented with 1% fetal bovine serum to mimic the physiological microenvironment. In ASCs, we screened for growth factors by semi-quantitative PCR and quantitative real-time PCR and found that the expression of bone morphogenetic protein 2 (BMP-2), vascular endothelial growth factor B (VEGFB), hypoxia inducible factor-1α (HIF-1α), fibroblast growth factor-2 (FGF-2), and transforming growth factor-β1 significantly increased after co-culture in comparison with mono-culture. In chondrocytes, VEGFA was significantly enhanced after co-culture. Unexpectedly, the expression of collagen II and aggrecan was significantly down-regulated in the co-culture group compared with the mono-culture group. Meanwhile, among all the growth factors screened, we found that the BMP family members BMP-2, BMP-4, and BMP-5 were down-regulated and that VEGFB, HIF-1α, FGF-2, and PDGF were significantly decreased after co-culture. These results suggest that crosstalk between ASCs and chondrocytes is a pathway through the regulated growth factors that might have potential in cartilage repair and regeneration and could be useful for tissue engineering. Nature Publishing Group 2016-02-03 /pmc/articles/PMC4738199/ /pubmed/26848404 http://dx.doi.org/10.1038/boneres.2015.36 Text en Copyright © 2016 Sichuan University http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhong, Juan
Guo, Bin
Xie, Jing
Deng, Shuwen
Fu, Na
Lin, Shiyu
Li, Guo
Lin, Yunfeng
Cai, Xiaoxiao
Crosstalk between adipose-derived stem cells and chondrocytes: when growth factors matter
title Crosstalk between adipose-derived stem cells and chondrocytes: when growth factors matter
title_full Crosstalk between adipose-derived stem cells and chondrocytes: when growth factors matter
title_fullStr Crosstalk between adipose-derived stem cells and chondrocytes: when growth factors matter
title_full_unstemmed Crosstalk between adipose-derived stem cells and chondrocytes: when growth factors matter
title_short Crosstalk between adipose-derived stem cells and chondrocytes: when growth factors matter
title_sort crosstalk between adipose-derived stem cells and chondrocytes: when growth factors matter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738199/
https://www.ncbi.nlm.nih.gov/pubmed/26848404
http://dx.doi.org/10.1038/boneres.2015.36
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