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Circulating and tumor-infiltrating mucosal associated invariant T (MAIT) cells in colorectal cancer patients

Mucosal associated invariant T (MAIT) cells are important for immune defense against infectious pathogens and regulate the pathogenesis of various inflammatory diseases. However, their roles in the development of colorectal cancer (CRC) are still unclear. This study examined the phenotype, distribut...

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Autores principales: Ling, Limian, Lin, Yuyang, Zheng, Wenwen, Hong, Sen, Tang, Xiuqi, Zhao, Pingwei, Li, Ming, Ni, Jingsong, Li, Chenguang, Wang, Lei, Jiang, Yanfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738248/
https://www.ncbi.nlm.nih.gov/pubmed/26837580
http://dx.doi.org/10.1038/srep20358
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author Ling, Limian
Lin, Yuyang
Zheng, Wenwen
Hong, Sen
Tang, Xiuqi
Zhao, Pingwei
Li, Ming
Ni, Jingsong
Li, Chenguang
Wang, Lei
Jiang, Yanfang
author_facet Ling, Limian
Lin, Yuyang
Zheng, Wenwen
Hong, Sen
Tang, Xiuqi
Zhao, Pingwei
Li, Ming
Ni, Jingsong
Li, Chenguang
Wang, Lei
Jiang, Yanfang
author_sort Ling, Limian
collection PubMed
description Mucosal associated invariant T (MAIT) cells are important for immune defense against infectious pathogens and regulate the pathogenesis of various inflammatory diseases. However, their roles in the development of colorectal cancer (CRC) are still unclear. This study examined the phenotype, distribution, clinical relevance and potential function of MAIT cells in CRC patients. We found that the percentages of circulating memory CD8(+) MAIT cells were significantly reduced while tumor infiltrating MAIT cells were increased, especially in patients with advanced CRC. The serum CEA levels were positively correlated with the percentages of tumor infiltrating MAIT cells in CRC patients, but negatively correlated with the percentages of circulating MAIT in advanced CRC patients. Activated circulating MAIT cells from CRC patients produced lower IFN-γ, but higher IL-17. Furthermore, higher levels of Vα7.2-Jα33, IFN-γ and IL-17A were expressed in the CRC tissues. Co-culture of activated MAIT cells with HCT116 cells enhanced IL-17 expression and induced HCT116 cell cycle arrest at G2/M phase in a contact- and dose-dependent manner, which was abrogated by treatment with anti-MR1. Therefore, MAIT cells preferably infiltrate into the solid tumor in CRC patients and may participate in the immune surveillance of CRC.
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spelling pubmed-47382482016-02-09 Circulating and tumor-infiltrating mucosal associated invariant T (MAIT) cells in colorectal cancer patients Ling, Limian Lin, Yuyang Zheng, Wenwen Hong, Sen Tang, Xiuqi Zhao, Pingwei Li, Ming Ni, Jingsong Li, Chenguang Wang, Lei Jiang, Yanfang Sci Rep Article Mucosal associated invariant T (MAIT) cells are important for immune defense against infectious pathogens and regulate the pathogenesis of various inflammatory diseases. However, their roles in the development of colorectal cancer (CRC) are still unclear. This study examined the phenotype, distribution, clinical relevance and potential function of MAIT cells in CRC patients. We found that the percentages of circulating memory CD8(+) MAIT cells were significantly reduced while tumor infiltrating MAIT cells were increased, especially in patients with advanced CRC. The serum CEA levels were positively correlated with the percentages of tumor infiltrating MAIT cells in CRC patients, but negatively correlated with the percentages of circulating MAIT in advanced CRC patients. Activated circulating MAIT cells from CRC patients produced lower IFN-γ, but higher IL-17. Furthermore, higher levels of Vα7.2-Jα33, IFN-γ and IL-17A were expressed in the CRC tissues. Co-culture of activated MAIT cells with HCT116 cells enhanced IL-17 expression and induced HCT116 cell cycle arrest at G2/M phase in a contact- and dose-dependent manner, which was abrogated by treatment with anti-MR1. Therefore, MAIT cells preferably infiltrate into the solid tumor in CRC patients and may participate in the immune surveillance of CRC. Nature Publishing Group 2016-02-03 /pmc/articles/PMC4738248/ /pubmed/26837580 http://dx.doi.org/10.1038/srep20358 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ling, Limian
Lin, Yuyang
Zheng, Wenwen
Hong, Sen
Tang, Xiuqi
Zhao, Pingwei
Li, Ming
Ni, Jingsong
Li, Chenguang
Wang, Lei
Jiang, Yanfang
Circulating and tumor-infiltrating mucosal associated invariant T (MAIT) cells in colorectal cancer patients
title Circulating and tumor-infiltrating mucosal associated invariant T (MAIT) cells in colorectal cancer patients
title_full Circulating and tumor-infiltrating mucosal associated invariant T (MAIT) cells in colorectal cancer patients
title_fullStr Circulating and tumor-infiltrating mucosal associated invariant T (MAIT) cells in colorectal cancer patients
title_full_unstemmed Circulating and tumor-infiltrating mucosal associated invariant T (MAIT) cells in colorectal cancer patients
title_short Circulating and tumor-infiltrating mucosal associated invariant T (MAIT) cells in colorectal cancer patients
title_sort circulating and tumor-infiltrating mucosal associated invariant t (mait) cells in colorectal cancer patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738248/
https://www.ncbi.nlm.nih.gov/pubmed/26837580
http://dx.doi.org/10.1038/srep20358
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