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Metabolism and Disposition of Aditoprim in Swine, Broilers, Carp and Rats

Aditoprim (ADP) is a newly developed antibacterial agent in veterinary medicine. The metabolism and disposition of ADP in swine, broilers, carp and rats were investigated by using a radio tracer method combined with a radioactivity detector and a liquid chromatography/ion trap/time-of-flight mass sp...

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Autores principales: Wang, Liye, Huang, Lingli, Pan, Yuanhu, Kuča, Kamil, Klímová, Blanka, Wu, Qinghua, Xie, Shuyu, Ahmad, Ijaz, Chen, Dongmei, Tao, Yanfei, Wan, Dan, Liu, Zhenli, Yuan, Zonghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738305/
https://www.ncbi.nlm.nih.gov/pubmed/26838160
http://dx.doi.org/10.1038/srep20370
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author Wang, Liye
Huang, Lingli
Pan, Yuanhu
Kuča, Kamil
Klímová, Blanka
Wu, Qinghua
Xie, Shuyu
Ahmad, Ijaz
Chen, Dongmei
Tao, Yanfei
Wan, Dan
Liu, Zhenli
Yuan, Zonghui
author_facet Wang, Liye
Huang, Lingli
Pan, Yuanhu
Kuča, Kamil
Klímová, Blanka
Wu, Qinghua
Xie, Shuyu
Ahmad, Ijaz
Chen, Dongmei
Tao, Yanfei
Wan, Dan
Liu, Zhenli
Yuan, Zonghui
author_sort Wang, Liye
collection PubMed
description Aditoprim (ADP) is a newly developed antibacterial agent in veterinary medicine. The metabolism and disposition of ADP in swine, broilers, carp and rats were investigated by using a radio tracer method combined with a radioactivity detector and a liquid chromatography/ion trap/time-of-flight mass spectrometry. After a single oral administration, more than 94% of the dose was recovered within 14 d in the four species. The urine excretion was dominant in swine and rats, making up 78% of the dose. N-monodesmethyl-ADP, N-didesmethyl-ADP, and 10 new metabolites were characterized. These metabolites were biotransformed from the process of demethylation, α-hydroxylation, N-oxidation, and NH(2)-glucuronidation. After an oral dose for 7 d, ADP-derived radioactivity was widely distributed in tissues, and high concentrations were especially observed in bile, liver, kidney, lung, and spleen. The radioactivity in the liver was eliminated much more slowly than in other tissues, with a half-life of 4.26, 3.38, 6.69, and 5.21 d in swine, broilers, carp, and rats, respectively. ADP, N-monodesmethyl-ADP, and N-didesmethyl-ADP were the major metabolites in edible tissues. Notably, ADP was detected with the highest concentration and the longest duration in these tissues. These findings indicated that ADP is the marker residue and the liver is the residue target tissue.
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spelling pubmed-47383052016-02-09 Metabolism and Disposition of Aditoprim in Swine, Broilers, Carp and Rats Wang, Liye Huang, Lingli Pan, Yuanhu Kuča, Kamil Klímová, Blanka Wu, Qinghua Xie, Shuyu Ahmad, Ijaz Chen, Dongmei Tao, Yanfei Wan, Dan Liu, Zhenli Yuan, Zonghui Sci Rep Article Aditoprim (ADP) is a newly developed antibacterial agent in veterinary medicine. The metabolism and disposition of ADP in swine, broilers, carp and rats were investigated by using a radio tracer method combined with a radioactivity detector and a liquid chromatography/ion trap/time-of-flight mass spectrometry. After a single oral administration, more than 94% of the dose was recovered within 14 d in the four species. The urine excretion was dominant in swine and rats, making up 78% of the dose. N-monodesmethyl-ADP, N-didesmethyl-ADP, and 10 new metabolites were characterized. These metabolites were biotransformed from the process of demethylation, α-hydroxylation, N-oxidation, and NH(2)-glucuronidation. After an oral dose for 7 d, ADP-derived radioactivity was widely distributed in tissues, and high concentrations were especially observed in bile, liver, kidney, lung, and spleen. The radioactivity in the liver was eliminated much more slowly than in other tissues, with a half-life of 4.26, 3.38, 6.69, and 5.21 d in swine, broilers, carp, and rats, respectively. ADP, N-monodesmethyl-ADP, and N-didesmethyl-ADP were the major metabolites in edible tissues. Notably, ADP was detected with the highest concentration and the longest duration in these tissues. These findings indicated that ADP is the marker residue and the liver is the residue target tissue. Nature Publishing Group 2016-02-03 /pmc/articles/PMC4738305/ /pubmed/26838160 http://dx.doi.org/10.1038/srep20370 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Liye
Huang, Lingli
Pan, Yuanhu
Kuča, Kamil
Klímová, Blanka
Wu, Qinghua
Xie, Shuyu
Ahmad, Ijaz
Chen, Dongmei
Tao, Yanfei
Wan, Dan
Liu, Zhenli
Yuan, Zonghui
Metabolism and Disposition of Aditoprim in Swine, Broilers, Carp and Rats
title Metabolism and Disposition of Aditoprim in Swine, Broilers, Carp and Rats
title_full Metabolism and Disposition of Aditoprim in Swine, Broilers, Carp and Rats
title_fullStr Metabolism and Disposition of Aditoprim in Swine, Broilers, Carp and Rats
title_full_unstemmed Metabolism and Disposition of Aditoprim in Swine, Broilers, Carp and Rats
title_short Metabolism and Disposition of Aditoprim in Swine, Broilers, Carp and Rats
title_sort metabolism and disposition of aditoprim in swine, broilers, carp and rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738305/
https://www.ncbi.nlm.nih.gov/pubmed/26838160
http://dx.doi.org/10.1038/srep20370
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