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MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation

The Escherichia coli SMC complex, MukBEF, forms clusters of molecules that interact with the decatenase topisomerase IV and which are normally associated with the chromosome replication origin region (ori). Here we demonstrate an additional ATP-hydrolysis-dependent association of MukBEF with the rep...

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Autores principales: Nolivos, Sophie, Upton, Amy L., Badrinarayanan, Anjana, Müller, Julius, Zawadzka, Katarzyna, Wiktor, Jakub, Gill, Amber, Arciszewska, Lidia, Nicolas, Emilien, Sherratt, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738335/
https://www.ncbi.nlm.nih.gov/pubmed/26818444
http://dx.doi.org/10.1038/ncomms10466
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author Nolivos, Sophie
Upton, Amy L.
Badrinarayanan, Anjana
Müller, Julius
Zawadzka, Katarzyna
Wiktor, Jakub
Gill, Amber
Arciszewska, Lidia
Nicolas, Emilien
Sherratt, David
author_facet Nolivos, Sophie
Upton, Amy L.
Badrinarayanan, Anjana
Müller, Julius
Zawadzka, Katarzyna
Wiktor, Jakub
Gill, Amber
Arciszewska, Lidia
Nicolas, Emilien
Sherratt, David
author_sort Nolivos, Sophie
collection PubMed
description The Escherichia coli SMC complex, MukBEF, forms clusters of molecules that interact with the decatenase topisomerase IV and which are normally associated with the chromosome replication origin region (ori). Here we demonstrate an additional ATP-hydrolysis-dependent association of MukBEF with the replication termination region (ter). Consistent with this, MukBEF interacts with MatP, which binds matS sites in ter. MatP displaces wild-type MukBEF complexes from ter, thereby facilitating their association with ori, and limiting the availability of topoisomerase IV (TopoIV) at ter. Displacement of MukBEF is impaired when MukB ATP hydrolysis is compromised and when MatP is absent, leading to a stable association of ter and MukBEF. Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP. We propose that the interplay between MukBEF and MatP directs chromosome organization in relation to MukBEF clusters and associated topisomerase IV, thereby ensuring timely chromosome unlinking and segregation.
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spelling pubmed-47383352016-03-04 MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation Nolivos, Sophie Upton, Amy L. Badrinarayanan, Anjana Müller, Julius Zawadzka, Katarzyna Wiktor, Jakub Gill, Amber Arciszewska, Lidia Nicolas, Emilien Sherratt, David Nat Commun Article The Escherichia coli SMC complex, MukBEF, forms clusters of molecules that interact with the decatenase topisomerase IV and which are normally associated with the chromosome replication origin region (ori). Here we demonstrate an additional ATP-hydrolysis-dependent association of MukBEF with the replication termination region (ter). Consistent with this, MukBEF interacts with MatP, which binds matS sites in ter. MatP displaces wild-type MukBEF complexes from ter, thereby facilitating their association with ori, and limiting the availability of topoisomerase IV (TopoIV) at ter. Displacement of MukBEF is impaired when MukB ATP hydrolysis is compromised and when MatP is absent, leading to a stable association of ter and MukBEF. Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP. We propose that the interplay between MukBEF and MatP directs chromosome organization in relation to MukBEF clusters and associated topisomerase IV, thereby ensuring timely chromosome unlinking and segregation. Nature Publishing Group 2016-01-28 /pmc/articles/PMC4738335/ /pubmed/26818444 http://dx.doi.org/10.1038/ncomms10466 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Nolivos, Sophie
Upton, Amy L.
Badrinarayanan, Anjana
Müller, Julius
Zawadzka, Katarzyna
Wiktor, Jakub
Gill, Amber
Arciszewska, Lidia
Nicolas, Emilien
Sherratt, David
MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation
title MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation
title_full MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation
title_fullStr MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation
title_full_unstemmed MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation
title_short MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation
title_sort matp regulates the coordinated action of topoisomerase iv and mukbef in chromosome segregation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738335/
https://www.ncbi.nlm.nih.gov/pubmed/26818444
http://dx.doi.org/10.1038/ncomms10466
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