Cargando…
MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation
The Escherichia coli SMC complex, MukBEF, forms clusters of molecules that interact with the decatenase topisomerase IV and which are normally associated with the chromosome replication origin region (ori). Here we demonstrate an additional ATP-hydrolysis-dependent association of MukBEF with the rep...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738335/ https://www.ncbi.nlm.nih.gov/pubmed/26818444 http://dx.doi.org/10.1038/ncomms10466 |
_version_ | 1782413592282267648 |
---|---|
author | Nolivos, Sophie Upton, Amy L. Badrinarayanan, Anjana Müller, Julius Zawadzka, Katarzyna Wiktor, Jakub Gill, Amber Arciszewska, Lidia Nicolas, Emilien Sherratt, David |
author_facet | Nolivos, Sophie Upton, Amy L. Badrinarayanan, Anjana Müller, Julius Zawadzka, Katarzyna Wiktor, Jakub Gill, Amber Arciszewska, Lidia Nicolas, Emilien Sherratt, David |
author_sort | Nolivos, Sophie |
collection | PubMed |
description | The Escherichia coli SMC complex, MukBEF, forms clusters of molecules that interact with the decatenase topisomerase IV and which are normally associated with the chromosome replication origin region (ori). Here we demonstrate an additional ATP-hydrolysis-dependent association of MukBEF with the replication termination region (ter). Consistent with this, MukBEF interacts with MatP, which binds matS sites in ter. MatP displaces wild-type MukBEF complexes from ter, thereby facilitating their association with ori, and limiting the availability of topoisomerase IV (TopoIV) at ter. Displacement of MukBEF is impaired when MukB ATP hydrolysis is compromised and when MatP is absent, leading to a stable association of ter and MukBEF. Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP. We propose that the interplay between MukBEF and MatP directs chromosome organization in relation to MukBEF clusters and associated topisomerase IV, thereby ensuring timely chromosome unlinking and segregation. |
format | Online Article Text |
id | pubmed-4738335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47383352016-03-04 MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation Nolivos, Sophie Upton, Amy L. Badrinarayanan, Anjana Müller, Julius Zawadzka, Katarzyna Wiktor, Jakub Gill, Amber Arciszewska, Lidia Nicolas, Emilien Sherratt, David Nat Commun Article The Escherichia coli SMC complex, MukBEF, forms clusters of molecules that interact with the decatenase topisomerase IV and which are normally associated with the chromosome replication origin region (ori). Here we demonstrate an additional ATP-hydrolysis-dependent association of MukBEF with the replication termination region (ter). Consistent with this, MukBEF interacts with MatP, which binds matS sites in ter. MatP displaces wild-type MukBEF complexes from ter, thereby facilitating their association with ori, and limiting the availability of topoisomerase IV (TopoIV) at ter. Displacement of MukBEF is impaired when MukB ATP hydrolysis is compromised and when MatP is absent, leading to a stable association of ter and MukBEF. Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP. We propose that the interplay between MukBEF and MatP directs chromosome organization in relation to MukBEF clusters and associated topisomerase IV, thereby ensuring timely chromosome unlinking and segregation. Nature Publishing Group 2016-01-28 /pmc/articles/PMC4738335/ /pubmed/26818444 http://dx.doi.org/10.1038/ncomms10466 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Nolivos, Sophie Upton, Amy L. Badrinarayanan, Anjana Müller, Julius Zawadzka, Katarzyna Wiktor, Jakub Gill, Amber Arciszewska, Lidia Nicolas, Emilien Sherratt, David MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
title | MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
title_full | MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
title_fullStr | MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
title_full_unstemmed | MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
title_short | MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation |
title_sort | matp regulates the coordinated action of topoisomerase iv and mukbef in chromosome segregation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738335/ https://www.ncbi.nlm.nih.gov/pubmed/26818444 http://dx.doi.org/10.1038/ncomms10466 |
work_keys_str_mv | AT nolivossophie matpregulatesthecoordinatedactionoftopoisomeraseivandmukbefinchromosomesegregation AT uptonamyl matpregulatesthecoordinatedactionoftopoisomeraseivandmukbefinchromosomesegregation AT badrinarayanananjana matpregulatesthecoordinatedactionoftopoisomeraseivandmukbefinchromosomesegregation AT mullerjulius matpregulatesthecoordinatedactionoftopoisomeraseivandmukbefinchromosomesegregation AT zawadzkakatarzyna matpregulatesthecoordinatedactionoftopoisomeraseivandmukbefinchromosomesegregation AT wiktorjakub matpregulatesthecoordinatedactionoftopoisomeraseivandmukbefinchromosomesegregation AT gillamber matpregulatesthecoordinatedactionoftopoisomeraseivandmukbefinchromosomesegregation AT arciszewskalidia matpregulatesthecoordinatedactionoftopoisomeraseivandmukbefinchromosomesegregation AT nicolasemilien matpregulatesthecoordinatedactionoftopoisomeraseivandmukbefinchromosomesegregation AT sherrattdavid matpregulatesthecoordinatedactionoftopoisomeraseivandmukbefinchromosomesegregation |