Metal ion transport quantified by ICP-MS in intact cells

The use of ICP-MS to measure metal ion content in biological tissues offers a highly sensitive means to study metal-dependent physiological processes. Here we describe the application of ICP-MS to measure membrane transport of Rb and K ions by the Na,K-ATPase in mouse skeletal muscles and human red...

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Autores principales: Figueroa, Julio A. Landero, Stiner, Cory A., Radzyukevich, Tatiana L., Heiny, Judith A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738345/
https://www.ncbi.nlm.nih.gov/pubmed/26838181
http://dx.doi.org/10.1038/srep20551
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author Figueroa, Julio A. Landero
Stiner, Cory A.
Radzyukevich, Tatiana L.
Heiny, Judith A.
author_facet Figueroa, Julio A. Landero
Stiner, Cory A.
Radzyukevich, Tatiana L.
Heiny, Judith A.
author_sort Figueroa, Julio A. Landero
collection PubMed
description The use of ICP-MS to measure metal ion content in biological tissues offers a highly sensitive means to study metal-dependent physiological processes. Here we describe the application of ICP-MS to measure membrane transport of Rb and K ions by the Na,K-ATPase in mouse skeletal muscles and human red blood cells. The ICP-MS method provides greater precision and statistical power than possible with conventional tracer flux methods. The method is widely applicable to studies of other metal ion transporters and metal-dependent processes in a range of cell types and conditions.
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spelling pubmed-47383452016-02-09 Metal ion transport quantified by ICP-MS in intact cells Figueroa, Julio A. Landero Stiner, Cory A. Radzyukevich, Tatiana L. Heiny, Judith A. Sci Rep Article The use of ICP-MS to measure metal ion content in biological tissues offers a highly sensitive means to study metal-dependent physiological processes. Here we describe the application of ICP-MS to measure membrane transport of Rb and K ions by the Na,K-ATPase in mouse skeletal muscles and human red blood cells. The ICP-MS method provides greater precision and statistical power than possible with conventional tracer flux methods. The method is widely applicable to studies of other metal ion transporters and metal-dependent processes in a range of cell types and conditions. Nature Publishing Group 2016-02-03 /pmc/articles/PMC4738345/ /pubmed/26838181 http://dx.doi.org/10.1038/srep20551 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Figueroa, Julio A. Landero
Stiner, Cory A.
Radzyukevich, Tatiana L.
Heiny, Judith A.
Metal ion transport quantified by ICP-MS in intact cells
title Metal ion transport quantified by ICP-MS in intact cells
title_full Metal ion transport quantified by ICP-MS in intact cells
title_fullStr Metal ion transport quantified by ICP-MS in intact cells
title_full_unstemmed Metal ion transport quantified by ICP-MS in intact cells
title_short Metal ion transport quantified by ICP-MS in intact cells
title_sort metal ion transport quantified by icp-ms in intact cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738345/
https://www.ncbi.nlm.nih.gov/pubmed/26838181
http://dx.doi.org/10.1038/srep20551
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