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Pattern recognition receptor mediated downregulation of microRNA‐650 fine‐tunes MxA expression in dendritic cells infected with influenza A virus

MicroRNAs are important posttranscriptional regulators of gene expression, which have been shown to fine‐tune innate immune responses downstream of pattern recognition receptor (PRR) signaling. This study identifies miR‐650 as a novel PRR‐responsive microRNA that is downregulated upon stimulation of...

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Autores principales: Pichulik, Tica, Khatamzas, Elham, Liu, Xiao, Brain, Oliver, Delmiro Garcia, Magno, Leslie, Alasdair, Danis, Benedicte, Mayer, Alice, Baban, Dilair, Ragoussis, Jiannis, Weber, Alexander N. R., Simmons, Alison
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738369/
https://www.ncbi.nlm.nih.gov/pubmed/26460926
http://dx.doi.org/10.1002/eji.201444970
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author Pichulik, Tica
Khatamzas, Elham
Liu, Xiao
Brain, Oliver
Delmiro Garcia, Magno
Leslie, Alasdair
Danis, Benedicte
Mayer, Alice
Baban, Dilair
Ragoussis, Jiannis
Weber, Alexander N. R.
Simmons, Alison
author_facet Pichulik, Tica
Khatamzas, Elham
Liu, Xiao
Brain, Oliver
Delmiro Garcia, Magno
Leslie, Alasdair
Danis, Benedicte
Mayer, Alice
Baban, Dilair
Ragoussis, Jiannis
Weber, Alexander N. R.
Simmons, Alison
author_sort Pichulik, Tica
collection PubMed
description MicroRNAs are important posttranscriptional regulators of gene expression, which have been shown to fine‐tune innate immune responses downstream of pattern recognition receptor (PRR) signaling. This study identifies miR‐650 as a novel PRR‐responsive microRNA that is downregulated upon stimulation of primary human monocyte‐derived dendritic cells (MDDCs) with a variety of different microbe‐associated molecular patterns. A comprehensive target search combining in silico analysis, transcriptional profiling, and reporter assays reveals that miR‐650 regulates several well‐known interferon‐stimulated genes, including IFIT2 and MXA. In particular, downregulation of miR‐650 in influenza A infected MDDCs enhances the expression of MxA and may therefore contribute to the establishment of an antiviral state. Together these findings reveal a novel link between miR‐650 and the innate immune response in human MDDCs.
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spelling pubmed-47383692016-02-12 Pattern recognition receptor mediated downregulation of microRNA‐650 fine‐tunes MxA expression in dendritic cells infected with influenza A virus Pichulik, Tica Khatamzas, Elham Liu, Xiao Brain, Oliver Delmiro Garcia, Magno Leslie, Alasdair Danis, Benedicte Mayer, Alice Baban, Dilair Ragoussis, Jiannis Weber, Alexander N. R. Simmons, Alison Eur J Immunol Innate Immunity MicroRNAs are important posttranscriptional regulators of gene expression, which have been shown to fine‐tune innate immune responses downstream of pattern recognition receptor (PRR) signaling. This study identifies miR‐650 as a novel PRR‐responsive microRNA that is downregulated upon stimulation of primary human monocyte‐derived dendritic cells (MDDCs) with a variety of different microbe‐associated molecular patterns. A comprehensive target search combining in silico analysis, transcriptional profiling, and reporter assays reveals that miR‐650 regulates several well‐known interferon‐stimulated genes, including IFIT2 and MXA. In particular, downregulation of miR‐650 in influenza A infected MDDCs enhances the expression of MxA and may therefore contribute to the establishment of an antiviral state. Together these findings reveal a novel link between miR‐650 and the innate immune response in human MDDCs. John Wiley and Sons Inc. 2015-10-30 2016-01 /pmc/articles/PMC4738369/ /pubmed/26460926 http://dx.doi.org/10.1002/eji.201444970 Text en © 2015 The Authors. European Journal of Immunology published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Innate Immunity
Pichulik, Tica
Khatamzas, Elham
Liu, Xiao
Brain, Oliver
Delmiro Garcia, Magno
Leslie, Alasdair
Danis, Benedicte
Mayer, Alice
Baban, Dilair
Ragoussis, Jiannis
Weber, Alexander N. R.
Simmons, Alison
Pattern recognition receptor mediated downregulation of microRNA‐650 fine‐tunes MxA expression in dendritic cells infected with influenza A virus
title Pattern recognition receptor mediated downregulation of microRNA‐650 fine‐tunes MxA expression in dendritic cells infected with influenza A virus
title_full Pattern recognition receptor mediated downregulation of microRNA‐650 fine‐tunes MxA expression in dendritic cells infected with influenza A virus
title_fullStr Pattern recognition receptor mediated downregulation of microRNA‐650 fine‐tunes MxA expression in dendritic cells infected with influenza A virus
title_full_unstemmed Pattern recognition receptor mediated downregulation of microRNA‐650 fine‐tunes MxA expression in dendritic cells infected with influenza A virus
title_short Pattern recognition receptor mediated downregulation of microRNA‐650 fine‐tunes MxA expression in dendritic cells infected with influenza A virus
title_sort pattern recognition receptor mediated downregulation of microrna‐650 fine‐tunes mxa expression in dendritic cells infected with influenza a virus
topic Innate Immunity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4738369/
https://www.ncbi.nlm.nih.gov/pubmed/26460926
http://dx.doi.org/10.1002/eji.201444970
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