ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model

BACKGROUND: In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene. RESULTS: We successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has...

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Detalles Bibliográficos
Autores principales: Liu, Min, Lu, Tiantian, Jing, Fang, Dang, Suying, Zhang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739331/
https://www.ncbi.nlm.nih.gov/pubmed/26841794
http://dx.doi.org/10.1186/s12952-016-0045-0
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author Liu, Min
Lu, Tiantian
Jing, Fang
Dang, Suying
Zhang, Wei
author_facet Liu, Min
Lu, Tiantian
Jing, Fang
Dang, Suying
Zhang, Wei
author_sort Liu, Min
collection PubMed
description BACKGROUND: In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene. RESULTS: We successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Orthotopic inoculation of 4 T1 cells within the mammary fat pad of host mice, we found no significant difference in tumor growth and metastasis between Adamts-18 knockout mice and widetype control. CONCLUSIONS: We did not confirm that ADAMTS-18 in the host tissues is relevant for breast tumor progress in a murine 4 T1 breast cancer model.
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spelling pubmed-47393312016-02-04 ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model Liu, Min Lu, Tiantian Jing, Fang Dang, Suying Zhang, Wei J Negat Results Biomed Brief Report BACKGROUND: In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene. RESULTS: We successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Orthotopic inoculation of 4 T1 cells within the mammary fat pad of host mice, we found no significant difference in tumor growth and metastasis between Adamts-18 knockout mice and widetype control. CONCLUSIONS: We did not confirm that ADAMTS-18 in the host tissues is relevant for breast tumor progress in a murine 4 T1 breast cancer model. BioMed Central 2016-02-03 /pmc/articles/PMC4739331/ /pubmed/26841794 http://dx.doi.org/10.1186/s12952-016-0045-0 Text en © Liu et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Brief Report
Liu, Min
Lu, Tiantian
Jing, Fang
Dang, Suying
Zhang, Wei
ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model
title ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model
title_full ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model
title_fullStr ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model
title_full_unstemmed ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model
title_short ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model
title_sort adamts-18 in the host tissues exerts little effect on breast tumor progress in a murine 4t1 breast cancer model
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739331/
https://www.ncbi.nlm.nih.gov/pubmed/26841794
http://dx.doi.org/10.1186/s12952-016-0045-0
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