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A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene
Plasmids play critical roles in bacterial fitness and evolution of Pseudomonas aeruginosa. Here two plasmids found in a drug-resistant P. aeruginosa clinical isolate HS87 were completely sequenced. The pHS87b plasmid (11.2 kb) carries phage-related genes and function-unknown genes. Notably, pHS87b e...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739549/ https://www.ncbi.nlm.nih.gov/pubmed/26841043 http://dx.doi.org/10.1371/journal.pone.0148367 |
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author | Bi, Dexi Xie, Yingzhou Tai, Cui Jiang, Xiaofei Zhang, Jie Harrison, Ewan M. Jia, Shiru Deng, Zixin Rajakumar, Kumar Ou, Hong-Yu |
author_facet | Bi, Dexi Xie, Yingzhou Tai, Cui Jiang, Xiaofei Zhang, Jie Harrison, Ewan M. Jia, Shiru Deng, Zixin Rajakumar, Kumar Ou, Hong-Yu |
author_sort | Bi, Dexi |
collection | PubMed |
description | Plasmids play critical roles in bacterial fitness and evolution of Pseudomonas aeruginosa. Here two plasmids found in a drug-resistant P. aeruginosa clinical isolate HS87 were completely sequenced. The pHS87b plasmid (11.2 kb) carries phage-related genes and function-unknown genes. Notably, pHS87b encodes an integrase and has an adjacent tRNA(Thr)-associated attachment site. A corresponding integrated form of pHS87b at the tRNA(Thr) locus was identified on the chromosome of P. aeruginosa, showing that pHS87b is able to site-specifically integrate into the 3’-end of the tRNA(Thr) gene. The pHS87a plasmid (26.8 kb) displays a plastic structure containing a putative replication module, stability factors and a variable region. The RepA of pHS87a shows significant similarity to the replication proteins of pPT23A-family plasmids. pHS87a carries a transposon Tn6049, a truncated insertion sequence ΔIS1071 and a Tn402-like class 1 integron which contains an aacA4 cassette that may confer aminoglycoside resistance. Thus, pHS87b is a site-specific integrative plasmid whereas pHS87a is a plastic antibiotic resistance plasmid. The two native plasmids may promote the fitness and evolution of P. aeruginosa. |
format | Online Article Text |
id | pubmed-4739549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47395492016-02-11 A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene Bi, Dexi Xie, Yingzhou Tai, Cui Jiang, Xiaofei Zhang, Jie Harrison, Ewan M. Jia, Shiru Deng, Zixin Rajakumar, Kumar Ou, Hong-Yu PLoS One Research Article Plasmids play critical roles in bacterial fitness and evolution of Pseudomonas aeruginosa. Here two plasmids found in a drug-resistant P. aeruginosa clinical isolate HS87 were completely sequenced. The pHS87b plasmid (11.2 kb) carries phage-related genes and function-unknown genes. Notably, pHS87b encodes an integrase and has an adjacent tRNA(Thr)-associated attachment site. A corresponding integrated form of pHS87b at the tRNA(Thr) locus was identified on the chromosome of P. aeruginosa, showing that pHS87b is able to site-specifically integrate into the 3’-end of the tRNA(Thr) gene. The pHS87a plasmid (26.8 kb) displays a plastic structure containing a putative replication module, stability factors and a variable region. The RepA of pHS87a shows significant similarity to the replication proteins of pPT23A-family plasmids. pHS87a carries a transposon Tn6049, a truncated insertion sequence ΔIS1071 and a Tn402-like class 1 integron which contains an aacA4 cassette that may confer aminoglycoside resistance. Thus, pHS87b is a site-specific integrative plasmid whereas pHS87a is a plastic antibiotic resistance plasmid. The two native plasmids may promote the fitness and evolution of P. aeruginosa. Public Library of Science 2016-02-03 /pmc/articles/PMC4739549/ /pubmed/26841043 http://dx.doi.org/10.1371/journal.pone.0148367 Text en © 2016 Bi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bi, Dexi Xie, Yingzhou Tai, Cui Jiang, Xiaofei Zhang, Jie Harrison, Ewan M. Jia, Shiru Deng, Zixin Rajakumar, Kumar Ou, Hong-Yu A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene |
title | A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene |
title_full | A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene |
title_fullStr | A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene |
title_full_unstemmed | A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene |
title_short | A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene |
title_sort | site-specific integrative plasmid found in pseudomonas aeruginosa clinical isolate hs87 along with a plasmid carrying an aminoglycoside-resistant gene |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739549/ https://www.ncbi.nlm.nih.gov/pubmed/26841043 http://dx.doi.org/10.1371/journal.pone.0148367 |
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