Cargando…

A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene

Plasmids play critical roles in bacterial fitness and evolution of Pseudomonas aeruginosa. Here two plasmids found in a drug-resistant P. aeruginosa clinical isolate HS87 were completely sequenced. The pHS87b plasmid (11.2 kb) carries phage-related genes and function-unknown genes. Notably, pHS87b e...

Descripción completa

Detalles Bibliográficos
Autores principales: Bi, Dexi, Xie, Yingzhou, Tai, Cui, Jiang, Xiaofei, Zhang, Jie, Harrison, Ewan M., Jia, Shiru, Deng, Zixin, Rajakumar, Kumar, Ou, Hong-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739549/
https://www.ncbi.nlm.nih.gov/pubmed/26841043
http://dx.doi.org/10.1371/journal.pone.0148367
_version_ 1782413765670600704
author Bi, Dexi
Xie, Yingzhou
Tai, Cui
Jiang, Xiaofei
Zhang, Jie
Harrison, Ewan M.
Jia, Shiru
Deng, Zixin
Rajakumar, Kumar
Ou, Hong-Yu
author_facet Bi, Dexi
Xie, Yingzhou
Tai, Cui
Jiang, Xiaofei
Zhang, Jie
Harrison, Ewan M.
Jia, Shiru
Deng, Zixin
Rajakumar, Kumar
Ou, Hong-Yu
author_sort Bi, Dexi
collection PubMed
description Plasmids play critical roles in bacterial fitness and evolution of Pseudomonas aeruginosa. Here two plasmids found in a drug-resistant P. aeruginosa clinical isolate HS87 were completely sequenced. The pHS87b plasmid (11.2 kb) carries phage-related genes and function-unknown genes. Notably, pHS87b encodes an integrase and has an adjacent tRNA(Thr)-associated attachment site. A corresponding integrated form of pHS87b at the tRNA(Thr) locus was identified on the chromosome of P. aeruginosa, showing that pHS87b is able to site-specifically integrate into the 3’-end of the tRNA(Thr) gene. The pHS87a plasmid (26.8 kb) displays a plastic structure containing a putative replication module, stability factors and a variable region. The RepA of pHS87a shows significant similarity to the replication proteins of pPT23A-family plasmids. pHS87a carries a transposon Tn6049, a truncated insertion sequence ΔIS1071 and a Tn402-like class 1 integron which contains an aacA4 cassette that may confer aminoglycoside resistance. Thus, pHS87b is a site-specific integrative plasmid whereas pHS87a is a plastic antibiotic resistance plasmid. The two native plasmids may promote the fitness and evolution of P. aeruginosa.
format Online
Article
Text
id pubmed-4739549
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-47395492016-02-11 A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene Bi, Dexi Xie, Yingzhou Tai, Cui Jiang, Xiaofei Zhang, Jie Harrison, Ewan M. Jia, Shiru Deng, Zixin Rajakumar, Kumar Ou, Hong-Yu PLoS One Research Article Plasmids play critical roles in bacterial fitness and evolution of Pseudomonas aeruginosa. Here two plasmids found in a drug-resistant P. aeruginosa clinical isolate HS87 were completely sequenced. The pHS87b plasmid (11.2 kb) carries phage-related genes and function-unknown genes. Notably, pHS87b encodes an integrase and has an adjacent tRNA(Thr)-associated attachment site. A corresponding integrated form of pHS87b at the tRNA(Thr) locus was identified on the chromosome of P. aeruginosa, showing that pHS87b is able to site-specifically integrate into the 3’-end of the tRNA(Thr) gene. The pHS87a plasmid (26.8 kb) displays a plastic structure containing a putative replication module, stability factors and a variable region. The RepA of pHS87a shows significant similarity to the replication proteins of pPT23A-family plasmids. pHS87a carries a transposon Tn6049, a truncated insertion sequence ΔIS1071 and a Tn402-like class 1 integron which contains an aacA4 cassette that may confer aminoglycoside resistance. Thus, pHS87b is a site-specific integrative plasmid whereas pHS87a is a plastic antibiotic resistance plasmid. The two native plasmids may promote the fitness and evolution of P. aeruginosa. Public Library of Science 2016-02-03 /pmc/articles/PMC4739549/ /pubmed/26841043 http://dx.doi.org/10.1371/journal.pone.0148367 Text en © 2016 Bi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bi, Dexi
Xie, Yingzhou
Tai, Cui
Jiang, Xiaofei
Zhang, Jie
Harrison, Ewan M.
Jia, Shiru
Deng, Zixin
Rajakumar, Kumar
Ou, Hong-Yu
A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene
title A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene
title_full A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene
title_fullStr A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene
title_full_unstemmed A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene
title_short A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene
title_sort site-specific integrative plasmid found in pseudomonas aeruginosa clinical isolate hs87 along with a plasmid carrying an aminoglycoside-resistant gene
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739549/
https://www.ncbi.nlm.nih.gov/pubmed/26841043
http://dx.doi.org/10.1371/journal.pone.0148367
work_keys_str_mv AT bidexi asitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT xieyingzhou asitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT taicui asitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT jiangxiaofei asitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT zhangjie asitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT harrisonewanm asitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT jiashiru asitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT dengzixin asitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT rajakumarkumar asitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT ouhongyu asitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT bidexi sitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT xieyingzhou sitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT taicui sitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT jiangxiaofei sitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT zhangjie sitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT harrisonewanm sitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT jiashiru sitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT dengzixin sitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT rajakumarkumar sitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene
AT ouhongyu sitespecificintegrativeplasmidfoundinpseudomonasaeruginosaclinicalisolatehs87alongwithaplasmidcarryinganaminoglycosideresistantgene