Robust enumeration of cell subsets from tissue expression profiles

We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen, and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with r...

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Detalles Bibliográficos
Autores principales: Newman, Aaron M., Liu, Chih Long, Green, Michael R., Gentles, Andrew J., Feng, Weiguo, Xu, Yue, Hoang, Chuong D., Diehn, Maximilian, Alizadeh, Ash A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739640/
https://www.ncbi.nlm.nih.gov/pubmed/25822800
http://dx.doi.org/10.1038/nmeth.3337
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author Newman, Aaron M.
Liu, Chih Long
Green, Michael R.
Gentles, Andrew J.
Feng, Weiguo
Xu, Yue
Hoang, Chuong D.
Diehn, Maximilian
Alizadeh, Ash A.
author_facet Newman, Aaron M.
Liu, Chih Long
Green, Michael R.
Gentles, Andrew J.
Feng, Weiguo
Xu, Yue
Hoang, Chuong D.
Diehn, Maximilian
Alizadeh, Ash A.
author_sort Newman, Aaron M.
collection PubMed
description We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen, and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with respect to noise, unknown mixture content, and closely related cell types. CIBERSORT should enable large-scale analysis of RNA mixtures for cellular biomarkers and therapeutic targets (http://cibersort.stanford.edu).
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spelling pubmed-47396402016-02-03 Robust enumeration of cell subsets from tissue expression profiles Newman, Aaron M. Liu, Chih Long Green, Michael R. Gentles, Andrew J. Feng, Weiguo Xu, Yue Hoang, Chuong D. Diehn, Maximilian Alizadeh, Ash A. Nat Methods Article We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen, and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with respect to noise, unknown mixture content, and closely related cell types. CIBERSORT should enable large-scale analysis of RNA mixtures for cellular biomarkers and therapeutic targets (http://cibersort.stanford.edu). 2015-03-30 2015-05 /pmc/articles/PMC4739640/ /pubmed/25822800 http://dx.doi.org/10.1038/nmeth.3337 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Newman, Aaron M.
Liu, Chih Long
Green, Michael R.
Gentles, Andrew J.
Feng, Weiguo
Xu, Yue
Hoang, Chuong D.
Diehn, Maximilian
Alizadeh, Ash A.
Robust enumeration of cell subsets from tissue expression profiles
title Robust enumeration of cell subsets from tissue expression profiles
title_full Robust enumeration of cell subsets from tissue expression profiles
title_fullStr Robust enumeration of cell subsets from tissue expression profiles
title_full_unstemmed Robust enumeration of cell subsets from tissue expression profiles
title_short Robust enumeration of cell subsets from tissue expression profiles
title_sort robust enumeration of cell subsets from tissue expression profiles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739640/
https://www.ncbi.nlm.nih.gov/pubmed/25822800
http://dx.doi.org/10.1038/nmeth.3337
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