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Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils

BACKGROUND: Psammomys obesus gerbils are particularly prone to develop diabetes and obesity after brief period of abundant food intake. A hypercaloric high fat diet has been shown to affect cardiac function. Here, we sought to determine whether a short period of high fat feeding might alter myocardi...

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Autores principales: Sahraoui, Abdelhamid, Dewachter, Céline, de Medina, Geoffrey, Naeije, Robert, Aouichat Bouguerra, Souhila, Dewachter, Laurence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4740502/
https://www.ncbi.nlm.nih.gov/pubmed/26840416
http://dx.doi.org/10.1371/journal.pone.0148117
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author Sahraoui, Abdelhamid
Dewachter, Céline
de Medina, Geoffrey
Naeije, Robert
Aouichat Bouguerra, Souhila
Dewachter, Laurence
author_facet Sahraoui, Abdelhamid
Dewachter, Céline
de Medina, Geoffrey
Naeije, Robert
Aouichat Bouguerra, Souhila
Dewachter, Laurence
author_sort Sahraoui, Abdelhamid
collection PubMed
description BACKGROUND: Psammomys obesus gerbils are particularly prone to develop diabetes and obesity after brief period of abundant food intake. A hypercaloric high fat diet has been shown to affect cardiac function. Here, we sought to determine whether a short period of high fat feeding might alter myocardial structure and expression of calcium handling proteins in this particular strain of gerbils. METHODS: Twenty Psammomys obesus gerbils were randomly assigned to receive a normal plant diet (controls) or a high fat diet. At baseline and 16-week later, body weight, plasma biochemical parameters (including lipid and carbohydrate levels) were evaluated. Myocardial samples were collected for pathobiological evaluation. RESULTS: Sixteen-week high fat dieting resulted in body weight gain and hyperlipidemia, while levels of carbohydrates remained unchanged. At myocardial level, high fat diet induced structural disorganization, including cardiomyocyte hypertrophy, lipid accumulation, interstitial and perivascular fibrosis and increased number of infiltrating neutrophils. Myocardial expressions of pro-apoptotic Bax-to-Bcl-2 ratio, pro-inflammatory cytokines [interleukin (IL)-1β and tumor necrosis factor (TNF)-α], intercellular (ICAM1) and vascular adhesion molecules (VCAM1) increased, while gene encoding cardiac muscle protein, the alpha myosin heavy polypeptide (MYH6), was downregulated. Myocardial expressions of sarco(endo)plasmic calcium-ATPase (SERCA2) and voltage-dependent calcium channel (Cacna1c) decreased, while protein kinase A (PKA) and calcium-calmodulin-dependent protein kinase (CaMK2D) expressions increased. Myocardial expressions of ryanodine receptor, phospholamban and sodium/calcium exchanger (Slc8a1) did not change. CONCLUSIONS: We conclude that a relative short period of high fat diet in Psammomys obesus results in severe alterations of cardiac structure, activation of inflammatory and apoptotic processes, and altered expression of calcium-cycling determinants.
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spelling pubmed-47405022016-02-11 Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils Sahraoui, Abdelhamid Dewachter, Céline de Medina, Geoffrey Naeije, Robert Aouichat Bouguerra, Souhila Dewachter, Laurence PLoS One Research Article BACKGROUND: Psammomys obesus gerbils are particularly prone to develop diabetes and obesity after brief period of abundant food intake. A hypercaloric high fat diet has been shown to affect cardiac function. Here, we sought to determine whether a short period of high fat feeding might alter myocardial structure and expression of calcium handling proteins in this particular strain of gerbils. METHODS: Twenty Psammomys obesus gerbils were randomly assigned to receive a normal plant diet (controls) or a high fat diet. At baseline and 16-week later, body weight, plasma biochemical parameters (including lipid and carbohydrate levels) were evaluated. Myocardial samples were collected for pathobiological evaluation. RESULTS: Sixteen-week high fat dieting resulted in body weight gain and hyperlipidemia, while levels of carbohydrates remained unchanged. At myocardial level, high fat diet induced structural disorganization, including cardiomyocyte hypertrophy, lipid accumulation, interstitial and perivascular fibrosis and increased number of infiltrating neutrophils. Myocardial expressions of pro-apoptotic Bax-to-Bcl-2 ratio, pro-inflammatory cytokines [interleukin (IL)-1β and tumor necrosis factor (TNF)-α], intercellular (ICAM1) and vascular adhesion molecules (VCAM1) increased, while gene encoding cardiac muscle protein, the alpha myosin heavy polypeptide (MYH6), was downregulated. Myocardial expressions of sarco(endo)plasmic calcium-ATPase (SERCA2) and voltage-dependent calcium channel (Cacna1c) decreased, while protein kinase A (PKA) and calcium-calmodulin-dependent protein kinase (CaMK2D) expressions increased. Myocardial expressions of ryanodine receptor, phospholamban and sodium/calcium exchanger (Slc8a1) did not change. CONCLUSIONS: We conclude that a relative short period of high fat diet in Psammomys obesus results in severe alterations of cardiac structure, activation of inflammatory and apoptotic processes, and altered expression of calcium-cycling determinants. Public Library of Science 2016-02-03 /pmc/articles/PMC4740502/ /pubmed/26840416 http://dx.doi.org/10.1371/journal.pone.0148117 Text en © 2016 Sahraoui et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sahraoui, Abdelhamid
Dewachter, Céline
de Medina, Geoffrey
Naeije, Robert
Aouichat Bouguerra, Souhila
Dewachter, Laurence
Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils
title Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils
title_full Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils
title_fullStr Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils
title_full_unstemmed Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils
title_short Myocardial Structural and Biological Anomalies Induced by High Fat Diet in Psammomys obesus Gerbils
title_sort myocardial structural and biological anomalies induced by high fat diet in psammomys obesus gerbils
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4740502/
https://www.ncbi.nlm.nih.gov/pubmed/26840416
http://dx.doi.org/10.1371/journal.pone.0148117
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