Cargando…
Single Nucleotide Polymorphisms in IL17A and IL6 Are Associated with Decreased Risk for Pulmonary Tuberculosis in Southern Brazilian Population
In Mycobacterium tuberculosis (MTB) infection, the complex interaction of host immune system and the mycobacteria is associated with levels of cytokines production that play a major role in determining the outcome of the disease. Several single-nucleotide polymorphisms (SNPs) in cytokine genes have...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4740512/ https://www.ncbi.nlm.nih.gov/pubmed/26840977 http://dx.doi.org/10.1371/journal.pone.0147814 |
_version_ | 1782413861679267840 |
---|---|
author | Milano, Mariana Moraes, Milton Ozório Rodenbusch, Rodrigo Carvalho, Caroline Xavier Delcroix, Melaine Mousquer, Gabriel Laux da Costa, Lucas Unis, Gisela Dalla Costa, Elis Regina Rossetti, Maria Lucia Rosa |
author_facet | Milano, Mariana Moraes, Milton Ozório Rodenbusch, Rodrigo Carvalho, Caroline Xavier Delcroix, Melaine Mousquer, Gabriel Laux da Costa, Lucas Unis, Gisela Dalla Costa, Elis Regina Rossetti, Maria Lucia Rosa |
author_sort | Milano, Mariana |
collection | PubMed |
description | In Mycobacterium tuberculosis (MTB) infection, the complex interaction of host immune system and the mycobacteria is associated with levels of cytokines production that play a major role in determining the outcome of the disease. Several single-nucleotide polymorphisms (SNPs) in cytokine genes have been associated with tuberculosis (TB) outcome. The aim of this study was to evaluate the association between previously reported SNPs IL2–330 T>G (rs2069762); IL4–590 C>T (rs2243250); IL6–174 G>C (rs1800795); IL10–592 A>C (rs1800872); IL10–1082 G>A (rs1800896); IL17A -692 C>T (rs8193036); IL17A -197 G>A (rs2275913); TNF -238 G>A (rs361525); TNF -308 G>A (rs1800629) and IFNG +874 T>A (rs2430561) and pulmonary TB (PTB) susceptibility. We conducted a case-control study in individuals from Southern Brazil who were recruited between February 2012 and October 2013 in a high incidence TB city. We performed a multiplex genotyping assay in 191 patients with PTB and 175 healthy subjects. Our results suggest a decreased risk for PTB development associated with the IL17A -197A allele (OR = 0.29; p = 0.04), AA genotype (OR = 0.12; p = 0.04) and A carrier (AG/AA) (OR = 0.29; p = 0.004) and IL6 -174C carrier (CC/CG) (OR = 0.46; p = 0.04). We could not properly analyze IL17A -692 C>T (rs8193036) and IFNG +874T>A due to genotypic inconsistencies and found no evidence of association for the IL2, IL4, IL10 and TNF polymorphisms and PTB. In conclusion, our results show a protective effect of IL17 and IL6 polymorphisms on PTB outcome in Southern Brazilian population. |
format | Online Article Text |
id | pubmed-4740512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47405122016-02-11 Single Nucleotide Polymorphisms in IL17A and IL6 Are Associated with Decreased Risk for Pulmonary Tuberculosis in Southern Brazilian Population Milano, Mariana Moraes, Milton Ozório Rodenbusch, Rodrigo Carvalho, Caroline Xavier Delcroix, Melaine Mousquer, Gabriel Laux da Costa, Lucas Unis, Gisela Dalla Costa, Elis Regina Rossetti, Maria Lucia Rosa PLoS One Research Article In Mycobacterium tuberculosis (MTB) infection, the complex interaction of host immune system and the mycobacteria is associated with levels of cytokines production that play a major role in determining the outcome of the disease. Several single-nucleotide polymorphisms (SNPs) in cytokine genes have been associated with tuberculosis (TB) outcome. The aim of this study was to evaluate the association between previously reported SNPs IL2–330 T>G (rs2069762); IL4–590 C>T (rs2243250); IL6–174 G>C (rs1800795); IL10–592 A>C (rs1800872); IL10–1082 G>A (rs1800896); IL17A -692 C>T (rs8193036); IL17A -197 G>A (rs2275913); TNF -238 G>A (rs361525); TNF -308 G>A (rs1800629) and IFNG +874 T>A (rs2430561) and pulmonary TB (PTB) susceptibility. We conducted a case-control study in individuals from Southern Brazil who were recruited between February 2012 and October 2013 in a high incidence TB city. We performed a multiplex genotyping assay in 191 patients with PTB and 175 healthy subjects. Our results suggest a decreased risk for PTB development associated with the IL17A -197A allele (OR = 0.29; p = 0.04), AA genotype (OR = 0.12; p = 0.04) and A carrier (AG/AA) (OR = 0.29; p = 0.004) and IL6 -174C carrier (CC/CG) (OR = 0.46; p = 0.04). We could not properly analyze IL17A -692 C>T (rs8193036) and IFNG +874T>A due to genotypic inconsistencies and found no evidence of association for the IL2, IL4, IL10 and TNF polymorphisms and PTB. In conclusion, our results show a protective effect of IL17 and IL6 polymorphisms on PTB outcome in Southern Brazilian population. Public Library of Science 2016-02-03 /pmc/articles/PMC4740512/ /pubmed/26840977 http://dx.doi.org/10.1371/journal.pone.0147814 Text en © 2016 Milano et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Milano, Mariana Moraes, Milton Ozório Rodenbusch, Rodrigo Carvalho, Caroline Xavier Delcroix, Melaine Mousquer, Gabriel Laux da Costa, Lucas Unis, Gisela Dalla Costa, Elis Regina Rossetti, Maria Lucia Rosa Single Nucleotide Polymorphisms in IL17A and IL6 Are Associated with Decreased Risk for Pulmonary Tuberculosis in Southern Brazilian Population |
title | Single Nucleotide Polymorphisms in IL17A and IL6 Are Associated with Decreased Risk for Pulmonary Tuberculosis in Southern Brazilian Population |
title_full | Single Nucleotide Polymorphisms in IL17A and IL6 Are Associated with Decreased Risk for Pulmonary Tuberculosis in Southern Brazilian Population |
title_fullStr | Single Nucleotide Polymorphisms in IL17A and IL6 Are Associated with Decreased Risk for Pulmonary Tuberculosis in Southern Brazilian Population |
title_full_unstemmed | Single Nucleotide Polymorphisms in IL17A and IL6 Are Associated with Decreased Risk for Pulmonary Tuberculosis in Southern Brazilian Population |
title_short | Single Nucleotide Polymorphisms in IL17A and IL6 Are Associated with Decreased Risk for Pulmonary Tuberculosis in Southern Brazilian Population |
title_sort | single nucleotide polymorphisms in il17a and il6 are associated with decreased risk for pulmonary tuberculosis in southern brazilian population |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4740512/ https://www.ncbi.nlm.nih.gov/pubmed/26840977 http://dx.doi.org/10.1371/journal.pone.0147814 |
work_keys_str_mv | AT milanomariana singlenucleotidepolymorphismsinil17aandil6areassociatedwithdecreasedriskforpulmonarytuberculosisinsouthernbrazilianpopulation AT moraesmiltonozorio singlenucleotidepolymorphismsinil17aandil6areassociatedwithdecreasedriskforpulmonarytuberculosisinsouthernbrazilianpopulation AT rodenbuschrodrigo singlenucleotidepolymorphismsinil17aandil6areassociatedwithdecreasedriskforpulmonarytuberculosisinsouthernbrazilianpopulation AT carvalhocarolinexavier singlenucleotidepolymorphismsinil17aandil6areassociatedwithdecreasedriskforpulmonarytuberculosisinsouthernbrazilianpopulation AT delcroixmelaine singlenucleotidepolymorphismsinil17aandil6areassociatedwithdecreasedriskforpulmonarytuberculosisinsouthernbrazilianpopulation AT mousquergabriel singlenucleotidepolymorphismsinil17aandil6areassociatedwithdecreasedriskforpulmonarytuberculosisinsouthernbrazilianpopulation AT lauxdacostalucas singlenucleotidepolymorphismsinil17aandil6areassociatedwithdecreasedriskforpulmonarytuberculosisinsouthernbrazilianpopulation AT unisgisela singlenucleotidepolymorphismsinil17aandil6areassociatedwithdecreasedriskforpulmonarytuberculosisinsouthernbrazilianpopulation AT dallacostaelisregina singlenucleotidepolymorphismsinil17aandil6areassociatedwithdecreasedriskforpulmonarytuberculosisinsouthernbrazilianpopulation AT rossettimarialuciarosa singlenucleotidepolymorphismsinil17aandil6areassociatedwithdecreasedriskforpulmonarytuberculosisinsouthernbrazilianpopulation |