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Reconstruction of the insulin-like signalling pathway of Haemonchus contortus
BACKGROUND: In the present study, we reconstructed the insulin/insulin-like growth factor 1 signalling (IIS) pathway for Haemonchus contortus, which is one of the most important eukaryotic pathogens of livestock worldwide and is related to the free-living nematode Caenorhabditis elegans. METHODS: We...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741068/ https://www.ncbi.nlm.nih.gov/pubmed/26842675 http://dx.doi.org/10.1186/s13071-016-1341-8 |
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author | Mohandas, Namitha Hu, Min Stroehlein, Andreas J. Young, Neil D. Sternberg, Paul W. Lok, James B. Gasser, Robin B. |
author_facet | Mohandas, Namitha Hu, Min Stroehlein, Andreas J. Young, Neil D. Sternberg, Paul W. Lok, James B. Gasser, Robin B. |
author_sort | Mohandas, Namitha |
collection | PubMed |
description | BACKGROUND: In the present study, we reconstructed the insulin/insulin-like growth factor 1 signalling (IIS) pathway for Haemonchus contortus, which is one of the most important eukaryotic pathogens of livestock worldwide and is related to the free-living nematode Caenorhabditis elegans. METHODS: We curated full-length open-reading frames from assembled transcripts, defined the complement of genes that encode proteins involved in this pathway and then investigated the transcription profiles of these genes for all key developmental stages of H. contortus. RESULTS: The core components of the IIS pathway are similar to their respective homologs in C. elegans. However, there is considerable variation in the numbers of isoforms between H. contortus and C. elegans and an absence of AKT-2 and DDL-2 homologs from H. contortus. Interestingly, DAF-16 has a single isoform in H. contortus compared with 12 in C. elegans, suggesting novel functional roles in the parasitic nematode. Some IIS proteins, such as DAF-18 and SGK-1, vary in their functional domains, indicating distinct roles from their homologs in C. elegans. CONCLUSIONS: This study paves the way for the further characterization of key signalling pathways in other socioeconomically important parasites and should help understand the complex mechanisms involved in developmental processes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1341-8) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4741068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47410682016-02-05 Reconstruction of the insulin-like signalling pathway of Haemonchus contortus Mohandas, Namitha Hu, Min Stroehlein, Andreas J. Young, Neil D. Sternberg, Paul W. Lok, James B. Gasser, Robin B. Parasit Vectors Research BACKGROUND: In the present study, we reconstructed the insulin/insulin-like growth factor 1 signalling (IIS) pathway for Haemonchus contortus, which is one of the most important eukaryotic pathogens of livestock worldwide and is related to the free-living nematode Caenorhabditis elegans. METHODS: We curated full-length open-reading frames from assembled transcripts, defined the complement of genes that encode proteins involved in this pathway and then investigated the transcription profiles of these genes for all key developmental stages of H. contortus. RESULTS: The core components of the IIS pathway are similar to their respective homologs in C. elegans. However, there is considerable variation in the numbers of isoforms between H. contortus and C. elegans and an absence of AKT-2 and DDL-2 homologs from H. contortus. Interestingly, DAF-16 has a single isoform in H. contortus compared with 12 in C. elegans, suggesting novel functional roles in the parasitic nematode. Some IIS proteins, such as DAF-18 and SGK-1, vary in their functional domains, indicating distinct roles from their homologs in C. elegans. CONCLUSIONS: This study paves the way for the further characterization of key signalling pathways in other socioeconomically important parasites and should help understand the complex mechanisms involved in developmental processes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1341-8) contains supplementary material, which is available to authorized users. BioMed Central 2016-02-03 /pmc/articles/PMC4741068/ /pubmed/26842675 http://dx.doi.org/10.1186/s13071-016-1341-8 Text en © Mohandas et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Mohandas, Namitha Hu, Min Stroehlein, Andreas J. Young, Neil D. Sternberg, Paul W. Lok, James B. Gasser, Robin B. Reconstruction of the insulin-like signalling pathway of Haemonchus contortus |
title | Reconstruction of the insulin-like signalling pathway of Haemonchus contortus |
title_full | Reconstruction of the insulin-like signalling pathway of Haemonchus contortus |
title_fullStr | Reconstruction of the insulin-like signalling pathway of Haemonchus contortus |
title_full_unstemmed | Reconstruction of the insulin-like signalling pathway of Haemonchus contortus |
title_short | Reconstruction of the insulin-like signalling pathway of Haemonchus contortus |
title_sort | reconstruction of the insulin-like signalling pathway of haemonchus contortus |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741068/ https://www.ncbi.nlm.nih.gov/pubmed/26842675 http://dx.doi.org/10.1186/s13071-016-1341-8 |
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