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A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis

Experimental autoimmune encephalomyelitis (EAE) is a well-established mouse model for multiple sclerosis and is characterized by infiltration of mononuclear cells and demyelination within the central nervous system along with the clinical symptoms of paralysis. EAE is a multifocal and random disease...

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Autores principales: Gibson-Corley, Katherine N., Boyden, Alexander W., Leidinger, Mariah R., Lambertz, Allyn M., Ofori-Amanfo, Georgina, Naumann, Paul W., Goeken, J. Adam, Karandikar, Nitin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741089/
https://www.ncbi.nlm.nih.gov/pubmed/26855861
http://dx.doi.org/10.7717/peerj.1600
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author Gibson-Corley, Katherine N.
Boyden, Alexander W.
Leidinger, Mariah R.
Lambertz, Allyn M.
Ofori-Amanfo, Georgina
Naumann, Paul W.
Goeken, J. Adam
Karandikar, Nitin J.
author_facet Gibson-Corley, Katherine N.
Boyden, Alexander W.
Leidinger, Mariah R.
Lambertz, Allyn M.
Ofori-Amanfo, Georgina
Naumann, Paul W.
Goeken, J. Adam
Karandikar, Nitin J.
author_sort Gibson-Corley, Katherine N.
collection PubMed
description Experimental autoimmune encephalomyelitis (EAE) is a well-established mouse model for multiple sclerosis and is characterized by infiltration of mononuclear cells and demyelination within the central nervous system along with the clinical symptoms of paralysis. EAE is a multifocal and random disease, which sometimes makes histopathologic analysis of lesions difficult as it may not be possible to predict where lesions will occur, especially when evaluating cross sections of spinal cord. Consequently, lesions may be easily missed due to limited sampling in traditional approaches. To evaluate the entire length of the spinal cord while maintaining anatomic integrity, we have developed a method to section the cord within the decalcified spinal column, which allows for the study of the multifocal nature of this disease and also minimizes handling artifact. HE and Luxol fast blue staining of these spinal cord sections revealed a paucity of lesions in some areas, while others showed marked inflammation and demyelination. The percentage of spinal cord affected by EAE was evaluated at four separate areas of longitudinally sectioned cord and it varied greatly within each animal. Immunohistochemical staining of in situ spinal cords which had undergone decalcification was successful for key immuno-markers used in EAE research including CD3 for T cells, B220 for B cells and F4/80 for murine macrophages. This method will allow investigators to look at the entire spinal cord on a single slide and evaluate the spinal cord with and without classic EAE lesions.
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spelling pubmed-47410892016-02-05 A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis Gibson-Corley, Katherine N. Boyden, Alexander W. Leidinger, Mariah R. Lambertz, Allyn M. Ofori-Amanfo, Georgina Naumann, Paul W. Goeken, J. Adam Karandikar, Nitin J. PeerJ Neurology Experimental autoimmune encephalomyelitis (EAE) is a well-established mouse model for multiple sclerosis and is characterized by infiltration of mononuclear cells and demyelination within the central nervous system along with the clinical symptoms of paralysis. EAE is a multifocal and random disease, which sometimes makes histopathologic analysis of lesions difficult as it may not be possible to predict where lesions will occur, especially when evaluating cross sections of spinal cord. Consequently, lesions may be easily missed due to limited sampling in traditional approaches. To evaluate the entire length of the spinal cord while maintaining anatomic integrity, we have developed a method to section the cord within the decalcified spinal column, which allows for the study of the multifocal nature of this disease and also minimizes handling artifact. HE and Luxol fast blue staining of these spinal cord sections revealed a paucity of lesions in some areas, while others showed marked inflammation and demyelination. The percentage of spinal cord affected by EAE was evaluated at four separate areas of longitudinally sectioned cord and it varied greatly within each animal. Immunohistochemical staining of in situ spinal cords which had undergone decalcification was successful for key immuno-markers used in EAE research including CD3 for T cells, B220 for B cells and F4/80 for murine macrophages. This method will allow investigators to look at the entire spinal cord on a single slide and evaluate the spinal cord with and without classic EAE lesions. PeerJ Inc. 2016-01-26 /pmc/articles/PMC4741089/ /pubmed/26855861 http://dx.doi.org/10.7717/peerj.1600 Text en © 2016 Gibson-Corley et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Neurology
Gibson-Corley, Katherine N.
Boyden, Alexander W.
Leidinger, Mariah R.
Lambertz, Allyn M.
Ofori-Amanfo, Georgina
Naumann, Paul W.
Goeken, J. Adam
Karandikar, Nitin J.
A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis
title A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis
title_full A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis
title_fullStr A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis
title_full_unstemmed A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis
title_short A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis
title_sort method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis
topic Neurology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741089/
https://www.ncbi.nlm.nih.gov/pubmed/26855861
http://dx.doi.org/10.7717/peerj.1600
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