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A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis
Experimental autoimmune encephalomyelitis (EAE) is a well-established mouse model for multiple sclerosis and is characterized by infiltration of mononuclear cells and demyelination within the central nervous system along with the clinical symptoms of paralysis. EAE is a multifocal and random disease...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741089/ https://www.ncbi.nlm.nih.gov/pubmed/26855861 http://dx.doi.org/10.7717/peerj.1600 |
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author | Gibson-Corley, Katherine N. Boyden, Alexander W. Leidinger, Mariah R. Lambertz, Allyn M. Ofori-Amanfo, Georgina Naumann, Paul W. Goeken, J. Adam Karandikar, Nitin J. |
author_facet | Gibson-Corley, Katherine N. Boyden, Alexander W. Leidinger, Mariah R. Lambertz, Allyn M. Ofori-Amanfo, Georgina Naumann, Paul W. Goeken, J. Adam Karandikar, Nitin J. |
author_sort | Gibson-Corley, Katherine N. |
collection | PubMed |
description | Experimental autoimmune encephalomyelitis (EAE) is a well-established mouse model for multiple sclerosis and is characterized by infiltration of mononuclear cells and demyelination within the central nervous system along with the clinical symptoms of paralysis. EAE is a multifocal and random disease, which sometimes makes histopathologic analysis of lesions difficult as it may not be possible to predict where lesions will occur, especially when evaluating cross sections of spinal cord. Consequently, lesions may be easily missed due to limited sampling in traditional approaches. To evaluate the entire length of the spinal cord while maintaining anatomic integrity, we have developed a method to section the cord within the decalcified spinal column, which allows for the study of the multifocal nature of this disease and also minimizes handling artifact. HE and Luxol fast blue staining of these spinal cord sections revealed a paucity of lesions in some areas, while others showed marked inflammation and demyelination. The percentage of spinal cord affected by EAE was evaluated at four separate areas of longitudinally sectioned cord and it varied greatly within each animal. Immunohistochemical staining of in situ spinal cords which had undergone decalcification was successful for key immuno-markers used in EAE research including CD3 for T cells, B220 for B cells and F4/80 for murine macrophages. This method will allow investigators to look at the entire spinal cord on a single slide and evaluate the spinal cord with and without classic EAE lesions. |
format | Online Article Text |
id | pubmed-4741089 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47410892016-02-05 A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis Gibson-Corley, Katherine N. Boyden, Alexander W. Leidinger, Mariah R. Lambertz, Allyn M. Ofori-Amanfo, Georgina Naumann, Paul W. Goeken, J. Adam Karandikar, Nitin J. PeerJ Neurology Experimental autoimmune encephalomyelitis (EAE) is a well-established mouse model for multiple sclerosis and is characterized by infiltration of mononuclear cells and demyelination within the central nervous system along with the clinical symptoms of paralysis. EAE is a multifocal and random disease, which sometimes makes histopathologic analysis of lesions difficult as it may not be possible to predict where lesions will occur, especially when evaluating cross sections of spinal cord. Consequently, lesions may be easily missed due to limited sampling in traditional approaches. To evaluate the entire length of the spinal cord while maintaining anatomic integrity, we have developed a method to section the cord within the decalcified spinal column, which allows for the study of the multifocal nature of this disease and also minimizes handling artifact. HE and Luxol fast blue staining of these spinal cord sections revealed a paucity of lesions in some areas, while others showed marked inflammation and demyelination. The percentage of spinal cord affected by EAE was evaluated at four separate areas of longitudinally sectioned cord and it varied greatly within each animal. Immunohistochemical staining of in situ spinal cords which had undergone decalcification was successful for key immuno-markers used in EAE research including CD3 for T cells, B220 for B cells and F4/80 for murine macrophages. This method will allow investigators to look at the entire spinal cord on a single slide and evaluate the spinal cord with and without classic EAE lesions. PeerJ Inc. 2016-01-26 /pmc/articles/PMC4741089/ /pubmed/26855861 http://dx.doi.org/10.7717/peerj.1600 Text en © 2016 Gibson-Corley et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Neurology Gibson-Corley, Katherine N. Boyden, Alexander W. Leidinger, Mariah R. Lambertz, Allyn M. Ofori-Amanfo, Georgina Naumann, Paul W. Goeken, J. Adam Karandikar, Nitin J. A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis |
title | A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis |
title_full | A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis |
title_fullStr | A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis |
title_full_unstemmed | A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis |
title_short | A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis |
title_sort | method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis |
topic | Neurology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741089/ https://www.ncbi.nlm.nih.gov/pubmed/26855861 http://dx.doi.org/10.7717/peerj.1600 |
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