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Abnormal expression of calcyphosine is associated with poor prognosis and cell biology function in colorectal cancer
The aim of this study was to investigate the calcyphosine (CAPS) expression in human colorectal cancer (CRC) and to explore its clinical and prognostic significances. CAPS expression was measured by Western blot, real-time polymerase chain reaction analysis, and immunohistochemistry. The relationshi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove Medical Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741367/ https://www.ncbi.nlm.nih.gov/pubmed/26889086 http://dx.doi.org/10.2147/OTT.S92226 |
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author | Shao, Weiwei Wang, Quhui Wang, Feiran Jiang, Yasu Xu, Meirong Xu, Junfei |
author_facet | Shao, Weiwei Wang, Quhui Wang, Feiran Jiang, Yasu Xu, Meirong Xu, Junfei |
author_sort | Shao, Weiwei |
collection | PubMed |
description | The aim of this study was to investigate the calcyphosine (CAPS) expression in human colorectal cancer (CRC) and to explore its clinical and prognostic significances. CAPS expression was measured by Western blot, real-time polymerase chain reaction analysis, and immunohistochemistry. The relationships between the CAPS expression levels and the clinicopathological factors were investigated. The Kaplan–Meier method and log-rank test were used to investigate the overall survival of the patients. Moreover, the effects of CAPS on biological roles of CRC cells were also evaluated by MTT assay, colony formation assay, and transwell assay. CAPS was significantly overexpressed in cancerous tissue and CRC cell lines compared with adjacent nontumor tissue and a normal human intestinal epithelial cell line. Overexpression of CAPS was significantly associated with histological grade (P=0.004), invasive depth (P<0.001), lymph node metastasis (P=0.003), tumor node metastasis stage (P=0.017), and distant metastasis (P=0.042). Furthermore, silencing of CAPS expression in CRC cells inhibited their proliferation, colony formation, migration, and invasion. Kaplan–Meier survival analysis showed that high CAPS expression might demonstrate poor prognosis in CRC patients. Cox regression analysis revealed that CAPS expression was an independent prognostic factor of CRC. Our data suggested that the upregulation of CAPS might play a role in the carcinogenesis and progression of CRC. CAPS could be used as a potential diagnostic factor and be an independent good prognostic indicator for CRC patients. |
format | Online Article Text |
id | pubmed-4741367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47413672016-02-17 Abnormal expression of calcyphosine is associated with poor prognosis and cell biology function in colorectal cancer Shao, Weiwei Wang, Quhui Wang, Feiran Jiang, Yasu Xu, Meirong Xu, Junfei Onco Targets Ther Original Research The aim of this study was to investigate the calcyphosine (CAPS) expression in human colorectal cancer (CRC) and to explore its clinical and prognostic significances. CAPS expression was measured by Western blot, real-time polymerase chain reaction analysis, and immunohistochemistry. The relationships between the CAPS expression levels and the clinicopathological factors were investigated. The Kaplan–Meier method and log-rank test were used to investigate the overall survival of the patients. Moreover, the effects of CAPS on biological roles of CRC cells were also evaluated by MTT assay, colony formation assay, and transwell assay. CAPS was significantly overexpressed in cancerous tissue and CRC cell lines compared with adjacent nontumor tissue and a normal human intestinal epithelial cell line. Overexpression of CAPS was significantly associated with histological grade (P=0.004), invasive depth (P<0.001), lymph node metastasis (P=0.003), tumor node metastasis stage (P=0.017), and distant metastasis (P=0.042). Furthermore, silencing of CAPS expression in CRC cells inhibited their proliferation, colony formation, migration, and invasion. Kaplan–Meier survival analysis showed that high CAPS expression might demonstrate poor prognosis in CRC patients. Cox regression analysis revealed that CAPS expression was an independent prognostic factor of CRC. Our data suggested that the upregulation of CAPS might play a role in the carcinogenesis and progression of CRC. CAPS could be used as a potential diagnostic factor and be an independent good prognostic indicator for CRC patients. Dove Medical Press 2016-01-25 /pmc/articles/PMC4741367/ /pubmed/26889086 http://dx.doi.org/10.2147/OTT.S92226 Text en © 2016 Shao et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Shao, Weiwei Wang, Quhui Wang, Feiran Jiang, Yasu Xu, Meirong Xu, Junfei Abnormal expression of calcyphosine is associated with poor prognosis and cell biology function in colorectal cancer |
title | Abnormal expression of calcyphosine is associated with poor prognosis and cell biology function in colorectal cancer |
title_full | Abnormal expression of calcyphosine is associated with poor prognosis and cell biology function in colorectal cancer |
title_fullStr | Abnormal expression of calcyphosine is associated with poor prognosis and cell biology function in colorectal cancer |
title_full_unstemmed | Abnormal expression of calcyphosine is associated with poor prognosis and cell biology function in colorectal cancer |
title_short | Abnormal expression of calcyphosine is associated with poor prognosis and cell biology function in colorectal cancer |
title_sort | abnormal expression of calcyphosine is associated with poor prognosis and cell biology function in colorectal cancer |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741367/ https://www.ncbi.nlm.nih.gov/pubmed/26889086 http://dx.doi.org/10.2147/OTT.S92226 |
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