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Development of a Reagentless Biosensor for Inorganic Phosphate, Applicable over a Wide Concentration Range
[Image: see text] A fluorescent reagentless biosensor for inorganic phosphate (P(i)), based on the E. coli PstS phosphate binding protein, was redesigned to allow measurements of higher P(i) concentrations and at low, substoichiometric concentrations of biosensor. This was achieved by weakening P(i)...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American
Chemical Society
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741387/ https://www.ncbi.nlm.nih.gov/pubmed/26199994 http://dx.doi.org/10.1021/acs.biochem.5b00449 |