Cargando…
CREB1-driven expression of miR-320a promotes mitophagy by down-regulating VDAC1 expression during serum starvation in cervical cancer cells
The altered expression of miRNAs in response to stresses contributes to cancer pathogenesis. However, little is known regarding the mechanism by which cellular stresses drive alterations in miRNA expression. Here, we found that serum starvation enhanced mitophagy by downregulating the mitophagy-asso...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741499/ https://www.ncbi.nlm.nih.gov/pubmed/26472185 |
_version_ | 1782414005064695808 |
---|---|
author | Li, Qin-qin Zhang, Le Wan, Hai-ying Liu, Min Li, Xin Tang, Hua |
author_facet | Li, Qin-qin Zhang, Le Wan, Hai-ying Liu, Min Li, Xin Tang, Hua |
author_sort | Li, Qin-qin |
collection | PubMed |
description | The altered expression of miRNAs in response to stresses contributes to cancer pathogenesis. However, little is known regarding the mechanism by which cellular stresses drive alterations in miRNA expression. Here, we found that serum starvation enhanced mitophagy by downregulating the mitophagy-associated protein voltage-dependent anion channel 1 (VDAC1) and by inducing the expression of miR-320a and the transcription factor cAMP responsive element binding protein 1(CREB1). Furthermore, we cloned the promoter of miR-320a and identified the core promoter of miR-320a in the upstream −16 to −130 region of pre-miR-320a. Moreover, CREB1 was found to bind to the promoter of miR-320a to activate its expression and to induce mitophagy during serum starvation. Collectively, our results reveal a new mechanism underlying serum starvation-induced mitophagy in which serum starvation induces CREB1 expression, in turn activating miR-320a expression, which then down-regulates VDAC1 expression to facilitate mitophagy. These findings may provide new insights into cancer cell survival in response to environmental stresses. |
format | Online Article Text |
id | pubmed-4741499 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-47414992016-03-15 CREB1-driven expression of miR-320a promotes mitophagy by down-regulating VDAC1 expression during serum starvation in cervical cancer cells Li, Qin-qin Zhang, Le Wan, Hai-ying Liu, Min Li, Xin Tang, Hua Oncotarget Research Paper The altered expression of miRNAs in response to stresses contributes to cancer pathogenesis. However, little is known regarding the mechanism by which cellular stresses drive alterations in miRNA expression. Here, we found that serum starvation enhanced mitophagy by downregulating the mitophagy-associated protein voltage-dependent anion channel 1 (VDAC1) and by inducing the expression of miR-320a and the transcription factor cAMP responsive element binding protein 1(CREB1). Furthermore, we cloned the promoter of miR-320a and identified the core promoter of miR-320a in the upstream −16 to −130 region of pre-miR-320a. Moreover, CREB1 was found to bind to the promoter of miR-320a to activate its expression and to induce mitophagy during serum starvation. Collectively, our results reveal a new mechanism underlying serum starvation-induced mitophagy in which serum starvation induces CREB1 expression, in turn activating miR-320a expression, which then down-regulates VDAC1 expression to facilitate mitophagy. These findings may provide new insights into cancer cell survival in response to environmental stresses. Impact Journals LLC 2015-10-14 /pmc/articles/PMC4741499/ /pubmed/26472185 Text en Copyright: © 2015 Li et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Li, Qin-qin Zhang, Le Wan, Hai-ying Liu, Min Li, Xin Tang, Hua CREB1-driven expression of miR-320a promotes mitophagy by down-regulating VDAC1 expression during serum starvation in cervical cancer cells |
title | CREB1-driven expression of miR-320a promotes mitophagy by down-regulating VDAC1 expression during serum starvation in cervical cancer cells |
title_full | CREB1-driven expression of miR-320a promotes mitophagy by down-regulating VDAC1 expression during serum starvation in cervical cancer cells |
title_fullStr | CREB1-driven expression of miR-320a promotes mitophagy by down-regulating VDAC1 expression during serum starvation in cervical cancer cells |
title_full_unstemmed | CREB1-driven expression of miR-320a promotes mitophagy by down-regulating VDAC1 expression during serum starvation in cervical cancer cells |
title_short | CREB1-driven expression of miR-320a promotes mitophagy by down-regulating VDAC1 expression during serum starvation in cervical cancer cells |
title_sort | creb1-driven expression of mir-320a promotes mitophagy by down-regulating vdac1 expression during serum starvation in cervical cancer cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741499/ https://www.ncbi.nlm.nih.gov/pubmed/26472185 |
work_keys_str_mv | AT liqinqin creb1drivenexpressionofmir320apromotesmitophagybydownregulatingvdac1expressionduringserumstarvationincervicalcancercells AT zhangle creb1drivenexpressionofmir320apromotesmitophagybydownregulatingvdac1expressionduringserumstarvationincervicalcancercells AT wanhaiying creb1drivenexpressionofmir320apromotesmitophagybydownregulatingvdac1expressionduringserumstarvationincervicalcancercells AT liumin creb1drivenexpressionofmir320apromotesmitophagybydownregulatingvdac1expressionduringserumstarvationincervicalcancercells AT lixin creb1drivenexpressionofmir320apromotesmitophagybydownregulatingvdac1expressionduringserumstarvationincervicalcancercells AT tanghua creb1drivenexpressionofmir320apromotesmitophagybydownregulatingvdac1expressionduringserumstarvationincervicalcancercells |