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Suppressing NRIP1 inhibits growth of breast cancer cells in vitro and in vivo
Earlier age at menarche is a major risk factor for breast cancer. Our previous study identified Nrip1 (also known as Rip140) as a candidate gene for delaying female sexual maturation (FSM) and found that knocking out Nrip1 could significantly delay FSM in mice. To investigate the effects of NRIP1 in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741857/ https://www.ncbi.nlm.nih.gov/pubmed/26492163 |
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author | Aziz, Moammir H. Chen, Xundi Zhang, Qi DeFrain, Chad Osland, Jared Luo, Yizhou Shi, Xin Yuan, Rong |
author_facet | Aziz, Moammir H. Chen, Xundi Zhang, Qi DeFrain, Chad Osland, Jared Luo, Yizhou Shi, Xin Yuan, Rong |
author_sort | Aziz, Moammir H. |
collection | PubMed |
description | Earlier age at menarche is a major risk factor for breast cancer. Our previous study identified Nrip1 (also known as Rip140) as a candidate gene for delaying female sexual maturation (FSM) and found that knocking out Nrip1 could significantly delay FSM in mice. To investigate the effects of NRIP1 in breast cancer we used human cell lines and tissue arrays along with an in vivo study of DMBA-induced carcinogenesis in Nrip1 knockout mice. Analysis of tissue arrays found that NRIP1 is elevated in tumors compared to cancer adjacent normal tissue. Interestingly, in benign tumors NRIP1 levels are higher in the cytosol of stromal cells, but NRIP1 levels are higher in the nuclei of epithelial cells in malignancies. We also found overexpression of NRIP1 in breast cancer cell lines, and that suppression of NRIP1 by siRNA in these cells significantly induced apoptosis and inhibited cell growth. Furthermore, in vivo data suggests that NRIP1 is upregulated in DMBA-induced breast cancer. Importantly, we found that DMBA-induced carcinogenesis is suppressed in Nrip1 knockdown mice. These findings suggest that NRIP1 plays a critical role in promoting the progression and development of breast cancer and that it may be a potential therapeutic target for the new breast cancer treatments. |
format | Online Article Text |
id | pubmed-4741857 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-47418572016-03-23 Suppressing NRIP1 inhibits growth of breast cancer cells in vitro and in vivo Aziz, Moammir H. Chen, Xundi Zhang, Qi DeFrain, Chad Osland, Jared Luo, Yizhou Shi, Xin Yuan, Rong Oncotarget Research Paper Earlier age at menarche is a major risk factor for breast cancer. Our previous study identified Nrip1 (also known as Rip140) as a candidate gene for delaying female sexual maturation (FSM) and found that knocking out Nrip1 could significantly delay FSM in mice. To investigate the effects of NRIP1 in breast cancer we used human cell lines and tissue arrays along with an in vivo study of DMBA-induced carcinogenesis in Nrip1 knockout mice. Analysis of tissue arrays found that NRIP1 is elevated in tumors compared to cancer adjacent normal tissue. Interestingly, in benign tumors NRIP1 levels are higher in the cytosol of stromal cells, but NRIP1 levels are higher in the nuclei of epithelial cells in malignancies. We also found overexpression of NRIP1 in breast cancer cell lines, and that suppression of NRIP1 by siRNA in these cells significantly induced apoptosis and inhibited cell growth. Furthermore, in vivo data suggests that NRIP1 is upregulated in DMBA-induced breast cancer. Importantly, we found that DMBA-induced carcinogenesis is suppressed in Nrip1 knockdown mice. These findings suggest that NRIP1 plays a critical role in promoting the progression and development of breast cancer and that it may be a potential therapeutic target for the new breast cancer treatments. Impact Journals LLC 2015-10-15 /pmc/articles/PMC4741857/ /pubmed/26492163 Text en Copyright: © 2015 Aziz et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Aziz, Moammir H. Chen, Xundi Zhang, Qi DeFrain, Chad Osland, Jared Luo, Yizhou Shi, Xin Yuan, Rong Suppressing NRIP1 inhibits growth of breast cancer cells in vitro and in vivo |
title | Suppressing NRIP1 inhibits growth of breast cancer cells in vitro and in vivo |
title_full | Suppressing NRIP1 inhibits growth of breast cancer cells in vitro and in vivo |
title_fullStr | Suppressing NRIP1 inhibits growth of breast cancer cells in vitro and in vivo |
title_full_unstemmed | Suppressing NRIP1 inhibits growth of breast cancer cells in vitro and in vivo |
title_short | Suppressing NRIP1 inhibits growth of breast cancer cells in vitro and in vivo |
title_sort | suppressing nrip1 inhibits growth of breast cancer cells in vitro and in vivo |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741857/ https://www.ncbi.nlm.nih.gov/pubmed/26492163 |
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