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Clinical value of integrated-signature miRNAs in colorectal cancer: miRNA expression profiling analysis and experimental validation

MicroRNA (miRNA) expression profiling of colorectal cancer (CRC) are often inconsistent among different studies. To determine candidate miRNA biomarkers for CRC, we performed an integrative analysis of miRNA expression profiling compared CRC tissues and paired neighboring noncancerous colorectal tis...

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Detalles Bibliográficos
Autores principales: Chen, XiangJian, Shi, KeQing, Wang, YuQun, Song, Mei, Zhou, Wu, Tu, HongXiang, Lin, Zhuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741947/
https://www.ncbi.nlm.nih.gov/pubmed/26462034
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author Chen, XiangJian
Shi, KeQing
Wang, YuQun
Song, Mei
Zhou, Wu
Tu, HongXiang
Lin, Zhuo
author_facet Chen, XiangJian
Shi, KeQing
Wang, YuQun
Song, Mei
Zhou, Wu
Tu, HongXiang
Lin, Zhuo
author_sort Chen, XiangJian
collection PubMed
description MicroRNA (miRNA) expression profiling of colorectal cancer (CRC) are often inconsistent among different studies. To determine candidate miRNA biomarkers for CRC, we performed an integrative analysis of miRNA expression profiling compared CRC tissues and paired neighboring noncancerous colorectal tissues. Using robust rank aggregation method, we identified a miRNA set of 10 integrated-signature miRNAs. In addition, the qRT-PCR validation demonstrated that 9 miRNAs were consistent dysregulated with the integrative analysis in CRC tissues, 4 miRNAs (miR-21-5p, miR-183-5p, miR-17-5p and miR-20a-5p) were up-regulated expression, and 5 miRNAs (miR-145-5p, miR-195-5p, miR-139-5p, miR-378a-5p and miR-143-3p) were down-regulated expression (all p < 0.05). Consistent with the initial analysis, 7 miRNAs were found to be significantly dysregulated in CRC tissues in TCGA data base, 4 miRNAs (miR-21-5p, miR-183-5p, miR-17-5p and miR-20a-5p) were significantly up-regulated expression, and 3 miRNAs (miR-145-5p, miR-139-5p and miR-378a-5p) were significantly down-regulated expression in CRC tissues (all p < 0.001). Furthermore, miR-17-5p (p = 0.011) and miR-20a-5p (p = 0.003) were up-regulated expression in the III/IV tumor stage, miR-145-5p (p = 0.028) and miR-195-5p (p = 0.001) were significantly increased expression with microscopic vascular invasion in CRC tissues, miR-17-5p (p = 0.037) and miR-145-5p (p = 0.023) were significantly increased expression with lymphovascular invasion. Moreover, Cox regression analysis of CRC patients in TCGA data base showed miR-20a-5p was correlated with survival (hazard ratio: 1.875, 95%CI: 1.088–3.232, p = 0.024). Hence, the finding of current study provides a basic implication of these miRNAs for further clinical application in CRC.
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spelling pubmed-47419472016-03-17 Clinical value of integrated-signature miRNAs in colorectal cancer: miRNA expression profiling analysis and experimental validation Chen, XiangJian Shi, KeQing Wang, YuQun Song, Mei Zhou, Wu Tu, HongXiang Lin, Zhuo Oncotarget Research Paper MicroRNA (miRNA) expression profiling of colorectal cancer (CRC) are often inconsistent among different studies. To determine candidate miRNA biomarkers for CRC, we performed an integrative analysis of miRNA expression profiling compared CRC tissues and paired neighboring noncancerous colorectal tissues. Using robust rank aggregation method, we identified a miRNA set of 10 integrated-signature miRNAs. In addition, the qRT-PCR validation demonstrated that 9 miRNAs were consistent dysregulated with the integrative analysis in CRC tissues, 4 miRNAs (miR-21-5p, miR-183-5p, miR-17-5p and miR-20a-5p) were up-regulated expression, and 5 miRNAs (miR-145-5p, miR-195-5p, miR-139-5p, miR-378a-5p and miR-143-3p) were down-regulated expression (all p < 0.05). Consistent with the initial analysis, 7 miRNAs were found to be significantly dysregulated in CRC tissues in TCGA data base, 4 miRNAs (miR-21-5p, miR-183-5p, miR-17-5p and miR-20a-5p) were significantly up-regulated expression, and 3 miRNAs (miR-145-5p, miR-139-5p and miR-378a-5p) were significantly down-regulated expression in CRC tissues (all p < 0.001). Furthermore, miR-17-5p (p = 0.011) and miR-20a-5p (p = 0.003) were up-regulated expression in the III/IV tumor stage, miR-145-5p (p = 0.028) and miR-195-5p (p = 0.001) were significantly increased expression with microscopic vascular invasion in CRC tissues, miR-17-5p (p = 0.037) and miR-145-5p (p = 0.023) were significantly increased expression with lymphovascular invasion. Moreover, Cox regression analysis of CRC patients in TCGA data base showed miR-20a-5p was correlated with survival (hazard ratio: 1.875, 95%CI: 1.088–3.232, p = 0.024). Hence, the finding of current study provides a basic implication of these miRNAs for further clinical application in CRC. Impact Journals LLC 2015-10-10 /pmc/articles/PMC4741947/ /pubmed/26462034 Text en Copyright: © 2015 Chen et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Chen, XiangJian
Shi, KeQing
Wang, YuQun
Song, Mei
Zhou, Wu
Tu, HongXiang
Lin, Zhuo
Clinical value of integrated-signature miRNAs in colorectal cancer: miRNA expression profiling analysis and experimental validation
title Clinical value of integrated-signature miRNAs in colorectal cancer: miRNA expression profiling analysis and experimental validation
title_full Clinical value of integrated-signature miRNAs in colorectal cancer: miRNA expression profiling analysis and experimental validation
title_fullStr Clinical value of integrated-signature miRNAs in colorectal cancer: miRNA expression profiling analysis and experimental validation
title_full_unstemmed Clinical value of integrated-signature miRNAs in colorectal cancer: miRNA expression profiling analysis and experimental validation
title_short Clinical value of integrated-signature miRNAs in colorectal cancer: miRNA expression profiling analysis and experimental validation
title_sort clinical value of integrated-signature mirnas in colorectal cancer: mirna expression profiling analysis and experimental validation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741947/
https://www.ncbi.nlm.nih.gov/pubmed/26462034
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