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miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1
miR-124 and miR-506 are reportedly down-regulated and associated with tumor progression in many cancers, but little is known about their intrinsic regulatory mechanisms in colorectal cancer (CRC). In this study, we found that the miR-124 and miR-506 levels were significantly lower in human CRC tissu...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741989/ https://www.ncbi.nlm.nih.gov/pubmed/26497367 |
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author | Chen, Zhiheng Liu, Shaojun Tian, Li Wu, Minghao Ai, Feiyan Tang, Wuliang Zhao, Lian Ding, Juan Zhang, Liyang Tang, Anliu |
author_facet | Chen, Zhiheng Liu, Shaojun Tian, Li Wu, Minghao Ai, Feiyan Tang, Wuliang Zhao, Lian Ding, Juan Zhang, Liyang Tang, Anliu |
author_sort | Chen, Zhiheng |
collection | PubMed |
description | miR-124 and miR-506 are reportedly down-regulated and associated with tumor progression in many cancers, but little is known about their intrinsic regulatory mechanisms in colorectal cancer (CRC). In this study, we found that the miR-124 and miR-506 levels were significantly lower in human CRC tissues than in controls, as indicated by qRT-PCR and in situ hybridization histochemistry. We also found that the overexpression of miR-124 or miR-506 inhibited tumor cell progression and increased sensitivity to chemotherapy in vitro. Increased miR-124 or miR-506 expression also inhibited tumor cell proliferation and invasion in vivo. Luciferase reporter assays and western blotting were used to determine the association between miR-124, miR-506 and their target genes, DNMTs. We further identified that miR-124 and miR-506 directly targeted DNMT3B and indirectly targeted DNMT1. The overexpression of miR-124 and miR-506 reduced global DNA methylation and restored the expression of E-cadherin, MGMT and P16. In conclusion, our data showed that miR-124 and miR-506 inhibit progression and increase sensitivity to chemotherapy by targeting DNMT3B and DNMT1 in CRC. These findings may provide novel avenues for the development of targeted therapies. |
format | Online Article Text |
id | pubmed-4741989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-47419892016-03-17 miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1 Chen, Zhiheng Liu, Shaojun Tian, Li Wu, Minghao Ai, Feiyan Tang, Wuliang Zhao, Lian Ding, Juan Zhang, Liyang Tang, Anliu Oncotarget Research Paper miR-124 and miR-506 are reportedly down-regulated and associated with tumor progression in many cancers, but little is known about their intrinsic regulatory mechanisms in colorectal cancer (CRC). In this study, we found that the miR-124 and miR-506 levels were significantly lower in human CRC tissues than in controls, as indicated by qRT-PCR and in situ hybridization histochemistry. We also found that the overexpression of miR-124 or miR-506 inhibited tumor cell progression and increased sensitivity to chemotherapy in vitro. Increased miR-124 or miR-506 expression also inhibited tumor cell proliferation and invasion in vivo. Luciferase reporter assays and western blotting were used to determine the association between miR-124, miR-506 and their target genes, DNMTs. We further identified that miR-124 and miR-506 directly targeted DNMT3B and indirectly targeted DNMT1. The overexpression of miR-124 and miR-506 reduced global DNA methylation and restored the expression of E-cadherin, MGMT and P16. In conclusion, our data showed that miR-124 and miR-506 inhibit progression and increase sensitivity to chemotherapy by targeting DNMT3B and DNMT1 in CRC. These findings may provide novel avenues for the development of targeted therapies. Impact Journals LLC 2015-10-15 /pmc/articles/PMC4741989/ /pubmed/26497367 Text en Copyright: © 2015 Chen et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Chen, Zhiheng Liu, Shaojun Tian, Li Wu, Minghao Ai, Feiyan Tang, Wuliang Zhao, Lian Ding, Juan Zhang, Liyang Tang, Anliu miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1 |
title | miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1 |
title_full | miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1 |
title_fullStr | miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1 |
title_full_unstemmed | miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1 |
title_short | miR-124 and miR-506 inhibit colorectal cancer progression by targeting DNMT3B and DNMT1 |
title_sort | mir-124 and mir-506 inhibit colorectal cancer progression by targeting dnmt3b and dnmt1 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741989/ https://www.ncbi.nlm.nih.gov/pubmed/26497367 |
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