Cargando…

Ideal number of biopsy tumor fragments for predicting HER2 status in gastric carcinoma resection specimens

Intratumoral heterogeneity of HER2 expression is common in gastric cancers and pose a challenge for identifying patients who would benefit from anti-HER2 therapy. The aim of this study is to compare HER2 expression in biopsy and resection specimens of gastric carcinoma by immunohistochemistry (IHC)...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahn, Sangjeong, Ahn, Soomin, Van Vrancken, Michael, Lee, Minju, Ha, Sang Yun, Lee, Hyuk, Min, Byung-Hoon, Lee, Jun Haeng, Kim, Jae J., Choi, Sunkyu, Jung, Sin-Ho, Choi, Min Gew, Lee, Jun-Ho, Sohn, Tae Sung, Bae, Jae Moon, Kim, Sung, Kim, Kyoung-Mee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742006/
https://www.ncbi.nlm.nih.gov/pubmed/26460823
_version_ 1782414119850213376
author Ahn, Sangjeong
Ahn, Soomin
Van Vrancken, Michael
Lee, Minju
Ha, Sang Yun
Lee, Hyuk
Min, Byung-Hoon
Lee, Jun Haeng
Kim, Jae J.
Choi, Sunkyu
Jung, Sin-Ho
Choi, Min Gew
Lee, Jun-Ho
Sohn, Tae Sung
Bae, Jae Moon
Kim, Sung
Kim, Kyoung-Mee
author_facet Ahn, Sangjeong
Ahn, Soomin
Van Vrancken, Michael
Lee, Minju
Ha, Sang Yun
Lee, Hyuk
Min, Byung-Hoon
Lee, Jun Haeng
Kim, Jae J.
Choi, Sunkyu
Jung, Sin-Ho
Choi, Min Gew
Lee, Jun-Ho
Sohn, Tae Sung
Bae, Jae Moon
Kim, Sung
Kim, Kyoung-Mee
author_sort Ahn, Sangjeong
collection PubMed
description Intratumoral heterogeneity of HER2 expression is common in gastric cancers and pose a challenge for identifying patients who would benefit from anti-HER2 therapy. The aim of this study is to compare HER2 expression in biopsy and resection specimens of gastric carcinoma by immunohistochemistry (IHC) and to find the ideal number of biopsy tumor fragments that can accurately predict HER2 overexpression in the corresponding surgically resected specimen. The HER2 IHC results of 702 paired biopsy and resection specimens of gastric cancer were compared. The mean number of biopsy fragments among all cases was 4.3 (range 1–11). HER2 was positive in 130 (18.5%) endoscopic biopsies and in 102 (14.5%) gastrectomy specimens. Intratumoral heterogeneity of HER2 was found in 80 (61.5%) biopsies and 70 (68.6%) resection specimens. Out of the 70 surgical specimens with intratumoral heterogeneity, 24 (34.3%) of the corresponding biopsies were categorized as negative (positive conversion). In the 86 (12.3%) discrepant cases, negative conversion was observed in 57 (66.3%) cases and positive conversion in 29 (33.7%). The fragment numbers were significantly correlated with the discrepancy of results and positive predictability (P = 0.0315 and P = 0.0052). ROC curve analysis and positive predictability showed that 4 fragments should be obtained to minimize the differences in HER2 scores between biopsy and resection specimen. In gastric carcinomas with discrepant HER2 results between biopsy and surgical resection specimens, intratumoral heterogeneity is common with most of them showing positive conversion. To predict HER2 status precisely, at least 4 biopsy fragments containing tumor cells are required.
format Online
Article
Text
id pubmed-4742006
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-47420062016-03-17 Ideal number of biopsy tumor fragments for predicting HER2 status in gastric carcinoma resection specimens Ahn, Sangjeong Ahn, Soomin Van Vrancken, Michael Lee, Minju Ha, Sang Yun Lee, Hyuk Min, Byung-Hoon Lee, Jun Haeng Kim, Jae J. Choi, Sunkyu Jung, Sin-Ho Choi, Min Gew Lee, Jun-Ho Sohn, Tae Sung Bae, Jae Moon Kim, Sung Kim, Kyoung-Mee Oncotarget Clinical Research Paper Intratumoral heterogeneity of HER2 expression is common in gastric cancers and pose a challenge for identifying patients who would benefit from anti-HER2 therapy. The aim of this study is to compare HER2 expression in biopsy and resection specimens of gastric carcinoma by immunohistochemistry (IHC) and to find the ideal number of biopsy tumor fragments that can accurately predict HER2 overexpression in the corresponding surgically resected specimen. The HER2 IHC results of 702 paired biopsy and resection specimens of gastric cancer were compared. The mean number of biopsy fragments among all cases was 4.3 (range 1–11). HER2 was positive in 130 (18.5%) endoscopic biopsies and in 102 (14.5%) gastrectomy specimens. Intratumoral heterogeneity of HER2 was found in 80 (61.5%) biopsies and 70 (68.6%) resection specimens. Out of the 70 surgical specimens with intratumoral heterogeneity, 24 (34.3%) of the corresponding biopsies were categorized as negative (positive conversion). In the 86 (12.3%) discrepant cases, negative conversion was observed in 57 (66.3%) cases and positive conversion in 29 (33.7%). The fragment numbers were significantly correlated with the discrepancy of results and positive predictability (P = 0.0315 and P = 0.0052). ROC curve analysis and positive predictability showed that 4 fragments should be obtained to minimize the differences in HER2 scores between biopsy and resection specimen. In gastric carcinomas with discrepant HER2 results between biopsy and surgical resection specimens, intratumoral heterogeneity is common with most of them showing positive conversion. To predict HER2 status precisely, at least 4 biopsy fragments containing tumor cells are required. Impact Journals LLC 2015-10-09 /pmc/articles/PMC4742006/ /pubmed/26460823 Text en Copyright: © 2015 Ahn et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Clinical Research Paper
Ahn, Sangjeong
Ahn, Soomin
Van Vrancken, Michael
Lee, Minju
Ha, Sang Yun
Lee, Hyuk
Min, Byung-Hoon
Lee, Jun Haeng
Kim, Jae J.
Choi, Sunkyu
Jung, Sin-Ho
Choi, Min Gew
Lee, Jun-Ho
Sohn, Tae Sung
Bae, Jae Moon
Kim, Sung
Kim, Kyoung-Mee
Ideal number of biopsy tumor fragments for predicting HER2 status in gastric carcinoma resection specimens
title Ideal number of biopsy tumor fragments for predicting HER2 status in gastric carcinoma resection specimens
title_full Ideal number of biopsy tumor fragments for predicting HER2 status in gastric carcinoma resection specimens
title_fullStr Ideal number of biopsy tumor fragments for predicting HER2 status in gastric carcinoma resection specimens
title_full_unstemmed Ideal number of biopsy tumor fragments for predicting HER2 status in gastric carcinoma resection specimens
title_short Ideal number of biopsy tumor fragments for predicting HER2 status in gastric carcinoma resection specimens
title_sort ideal number of biopsy tumor fragments for predicting her2 status in gastric carcinoma resection specimens
topic Clinical Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742006/
https://www.ncbi.nlm.nih.gov/pubmed/26460823
work_keys_str_mv AT ahnsangjeong idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT ahnsoomin idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT vanvranckenmichael idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT leeminju idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT hasangyun idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT leehyuk idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT minbyunghoon idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT leejunhaeng idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT kimjaej idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT choisunkyu idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT jungsinho idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT choimingew idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT leejunho idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT sohntaesung idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT baejaemoon idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT kimsung idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens
AT kimkyoungmee idealnumberofbiopsytumorfragmentsforpredictingher2statusingastriccarcinomaresectionspecimens