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Parvovirus B19 Replication and Expression in Differentiating Erythroid Progenitor Cells
The pathogenic Parvovirus B19 (B19V) is characterized by a strict adaptation to erythroid progenitor cells (EPCs), a heterogeneous population of differentiating cells with diverse phenotypic and functional properties. In our work, we studied the dynamics of B19V infection in EPCs in dependence on th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742074/ https://www.ncbi.nlm.nih.gov/pubmed/26845771 http://dx.doi.org/10.1371/journal.pone.0148547 |
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author | Bua, Gloria Manaresi, Elisabetta Bonvicini, Francesca Gallinella, Giorgio |
author_facet | Bua, Gloria Manaresi, Elisabetta Bonvicini, Francesca Gallinella, Giorgio |
author_sort | Bua, Gloria |
collection | PubMed |
description | The pathogenic Parvovirus B19 (B19V) is characterized by a strict adaptation to erythroid progenitor cells (EPCs), a heterogeneous population of differentiating cells with diverse phenotypic and functional properties. In our work, we studied the dynamics of B19V infection in EPCs in dependence on the cell differentiation stage, in terms of distribution of infected cells, synthesis of viral nucleic acids and production of infectious virus. EPCs at early differentiation stage led to an abortive infection, without viral genome replication and a very low transcriptional activity. EPCs at later stages were permissive, with highest levels of viral replicative activity at day 9 (+3.0 Log from 2 to 48 hpi) and lower levels at day 18 (+1.5 Log from 2 to 48 hpi). B19V DNA increment was in accordance with the percentage of cells positive to flow-FISH assay (41.4% at day 9, 1.1% at day 18). Quantitation of total RNA indicated a close association of genome replication and transcription with viral RNA accumulation within infected cells related to viral DNA increase during the course of infection. Analysis of the different classes of mRNAs revealed two distinct pattern of genome expression profile with a fine regulation in the frequency utilization of RNA processing signals: an early phase, when cleavage at the proximal site leading to a higher relative production of mRNA for NS protein, and a late phase, when cleavage at the distal site was more frequent leading to higher relative abundance of mRNA for VP and 11 kDA proteins. Infectious virus was released from cells at day 6–15, but not at day 18. Our results, providing a detailed description of B19V replication and expression profile in differentiating EPCs, highlight the very tight adaptation of B19V to a specific cellular target defined both by its erythroid lineage and its differentiation stage. |
format | Online Article Text |
id | pubmed-4742074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47420742016-02-11 Parvovirus B19 Replication and Expression in Differentiating Erythroid Progenitor Cells Bua, Gloria Manaresi, Elisabetta Bonvicini, Francesca Gallinella, Giorgio PLoS One Research Article The pathogenic Parvovirus B19 (B19V) is characterized by a strict adaptation to erythroid progenitor cells (EPCs), a heterogeneous population of differentiating cells with diverse phenotypic and functional properties. In our work, we studied the dynamics of B19V infection in EPCs in dependence on the cell differentiation stage, in terms of distribution of infected cells, synthesis of viral nucleic acids and production of infectious virus. EPCs at early differentiation stage led to an abortive infection, without viral genome replication and a very low transcriptional activity. EPCs at later stages were permissive, with highest levels of viral replicative activity at day 9 (+3.0 Log from 2 to 48 hpi) and lower levels at day 18 (+1.5 Log from 2 to 48 hpi). B19V DNA increment was in accordance with the percentage of cells positive to flow-FISH assay (41.4% at day 9, 1.1% at day 18). Quantitation of total RNA indicated a close association of genome replication and transcription with viral RNA accumulation within infected cells related to viral DNA increase during the course of infection. Analysis of the different classes of mRNAs revealed two distinct pattern of genome expression profile with a fine regulation in the frequency utilization of RNA processing signals: an early phase, when cleavage at the proximal site leading to a higher relative production of mRNA for NS protein, and a late phase, when cleavage at the distal site was more frequent leading to higher relative abundance of mRNA for VP and 11 kDA proteins. Infectious virus was released from cells at day 6–15, but not at day 18. Our results, providing a detailed description of B19V replication and expression profile in differentiating EPCs, highlight the very tight adaptation of B19V to a specific cellular target defined both by its erythroid lineage and its differentiation stage. Public Library of Science 2016-02-04 /pmc/articles/PMC4742074/ /pubmed/26845771 http://dx.doi.org/10.1371/journal.pone.0148547 Text en © 2016 Bua et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bua, Gloria Manaresi, Elisabetta Bonvicini, Francesca Gallinella, Giorgio Parvovirus B19 Replication and Expression in Differentiating Erythroid Progenitor Cells |
title | Parvovirus B19 Replication and Expression in Differentiating Erythroid Progenitor Cells |
title_full | Parvovirus B19 Replication and Expression in Differentiating Erythroid Progenitor Cells |
title_fullStr | Parvovirus B19 Replication and Expression in Differentiating Erythroid Progenitor Cells |
title_full_unstemmed | Parvovirus B19 Replication and Expression in Differentiating Erythroid Progenitor Cells |
title_short | Parvovirus B19 Replication and Expression in Differentiating Erythroid Progenitor Cells |
title_sort | parvovirus b19 replication and expression in differentiating erythroid progenitor cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742074/ https://www.ncbi.nlm.nih.gov/pubmed/26845771 http://dx.doi.org/10.1371/journal.pone.0148547 |
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