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Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli
Disulfide bonds occurred in majority of secreted protein. Formation of correct disulfide bonds are must for achieving native conformation, solubility and activity. Production of recombinant proteins containing disulfide bond for therapeutic, diagnostic and various other purposes is a challenging tas...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Springer Berlin Heidelberg
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742420/ https://www.ncbi.nlm.nih.gov/pubmed/28330113 http://dx.doi.org/10.1007/s13205-016-0397-7 |