Amino acid changes in PB2 and HA affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein

Influenza viruses that express reporter proteins are useful tools, but are often attenuated. Recently, we found that an influenza virus encoding the Venus fluorescent protein acquired two mutations in its PB2 and HA proteins upon mouse adaptation. Here, we demonstrate that the enhanced viral replica...

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Detalles Bibliográficos
Autores principales: Katsura, Hiroaki, Fukuyama, Satoshi, Watanabe, Shinji, Ozawa, Makoto, Neumann, Gabriele, Kawaoka, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742795/
https://www.ncbi.nlm.nih.gov/pubmed/26847414
http://dx.doi.org/10.1038/srep19933
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author Katsura, Hiroaki
Fukuyama, Satoshi
Watanabe, Shinji
Ozawa, Makoto
Neumann, Gabriele
Kawaoka, Yoshihiro
author_facet Katsura, Hiroaki
Fukuyama, Satoshi
Watanabe, Shinji
Ozawa, Makoto
Neumann, Gabriele
Kawaoka, Yoshihiro
author_sort Katsura, Hiroaki
collection PubMed
description Influenza viruses that express reporter proteins are useful tools, but are often attenuated. Recently, we found that an influenza virus encoding the Venus fluorescent protein acquired two mutations in its PB2 and HA proteins upon mouse adaptation. Here, we demonstrate that the enhanced viral replication and virulence in mice of this Venus-expressing influenza virus are primarily conferred by the PB2-E712D mutation, with only a minor contribution by the HA-T380A mutation.
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spelling pubmed-47427952016-02-09 Amino acid changes in PB2 and HA affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein Katsura, Hiroaki Fukuyama, Satoshi Watanabe, Shinji Ozawa, Makoto Neumann, Gabriele Kawaoka, Yoshihiro Sci Rep Article Influenza viruses that express reporter proteins are useful tools, but are often attenuated. Recently, we found that an influenza virus encoding the Venus fluorescent protein acquired two mutations in its PB2 and HA proteins upon mouse adaptation. Here, we demonstrate that the enhanced viral replication and virulence in mice of this Venus-expressing influenza virus are primarily conferred by the PB2-E712D mutation, with only a minor contribution by the HA-T380A mutation. Nature Publishing Group 2016-02-05 /pmc/articles/PMC4742795/ /pubmed/26847414 http://dx.doi.org/10.1038/srep19933 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Katsura, Hiroaki
Fukuyama, Satoshi
Watanabe, Shinji
Ozawa, Makoto
Neumann, Gabriele
Kawaoka, Yoshihiro
Amino acid changes in PB2 and HA affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein
title Amino acid changes in PB2 and HA affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein
title_full Amino acid changes in PB2 and HA affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein
title_fullStr Amino acid changes in PB2 and HA affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein
title_full_unstemmed Amino acid changes in PB2 and HA affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein
title_short Amino acid changes in PB2 and HA affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein
title_sort amino acid changes in pb2 and ha affect the growth of a recombinant influenza virus expressing a fluorescent reporter protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742795/
https://www.ncbi.nlm.nih.gov/pubmed/26847414
http://dx.doi.org/10.1038/srep19933
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