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Lysing bloom-causing alga Phaeocystis globosa with microbial algicide: An efficient process that decreases the toxicity of algal exudates

Algicidal microbes could effectively remove the harmful algae from the waters. In this study, we were concerned with the ecological influence of an algicide extracted from Streptomyces alboflavus RPS, which could completely lyse the Phaeocystis globosa cells within two days. In microcosms, 4 μg/mL o...

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Autores principales: Cai, Guanjing, Yang, Xujun, Lai, Qiliang, Yu, Xiaoqi, Zhang, Huajun, Li, Yi, Chen, Zhangran, Lei, Xueqian, Zheng, Wei, Xu, Hong, Zheng, Tianling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742815/
https://www.ncbi.nlm.nih.gov/pubmed/26847810
http://dx.doi.org/10.1038/srep20081
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author Cai, Guanjing
Yang, Xujun
Lai, Qiliang
Yu, Xiaoqi
Zhang, Huajun
Li, Yi
Chen, Zhangran
Lei, Xueqian
Zheng, Wei
Xu, Hong
Zheng, Tianling
author_facet Cai, Guanjing
Yang, Xujun
Lai, Qiliang
Yu, Xiaoqi
Zhang, Huajun
Li, Yi
Chen, Zhangran
Lei, Xueqian
Zheng, Wei
Xu, Hong
Zheng, Tianling
author_sort Cai, Guanjing
collection PubMed
description Algicidal microbes could effectively remove the harmful algae from the waters. In this study, we were concerned with the ecological influence of an algicide extracted from Streptomyces alboflavus RPS, which could completely lyse the Phaeocystis globosa cells within two days. In microcosms, 4 μg/mL of the microbial algicide could efficiently remove P. globosa cells without suppressing other aquatic organisms. Bioluminescent assays confirmed that the toxicity of microbial algicide at this concentration was negligible. Interestingly, the toxicity of P. globosa exudates was also significantly reduced after being treated with the algicide. Further experiments revealed that the microbial algicide could instantly increase the permeability of the plasma membrane and disturb the photosynthetic system, followed by the deformation of organelles, vacuolization and increasing oxidative stress. The pre-incubation of N-acetyl cysteine (NAC) verified that the rapid damages to the plasma membrane and photosynthetic system caused the algal death in the early phase, and the increasing oxidative stress killed the rest. The late accumulation and possible release of CAT also explained the decreasing toxicity of the algal culture. These results indicated that this microbial algicide has great potential in controlling the growth of P. globosa on site.
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spelling pubmed-47428152016-02-09 Lysing bloom-causing alga Phaeocystis globosa with microbial algicide: An efficient process that decreases the toxicity of algal exudates Cai, Guanjing Yang, Xujun Lai, Qiliang Yu, Xiaoqi Zhang, Huajun Li, Yi Chen, Zhangran Lei, Xueqian Zheng, Wei Xu, Hong Zheng, Tianling Sci Rep Article Algicidal microbes could effectively remove the harmful algae from the waters. In this study, we were concerned with the ecological influence of an algicide extracted from Streptomyces alboflavus RPS, which could completely lyse the Phaeocystis globosa cells within two days. In microcosms, 4 μg/mL of the microbial algicide could efficiently remove P. globosa cells without suppressing other aquatic organisms. Bioluminescent assays confirmed that the toxicity of microbial algicide at this concentration was negligible. Interestingly, the toxicity of P. globosa exudates was also significantly reduced after being treated with the algicide. Further experiments revealed that the microbial algicide could instantly increase the permeability of the plasma membrane and disturb the photosynthetic system, followed by the deformation of organelles, vacuolization and increasing oxidative stress. The pre-incubation of N-acetyl cysteine (NAC) verified that the rapid damages to the plasma membrane and photosynthetic system caused the algal death in the early phase, and the increasing oxidative stress killed the rest. The late accumulation and possible release of CAT also explained the decreasing toxicity of the algal culture. These results indicated that this microbial algicide has great potential in controlling the growth of P. globosa on site. Nature Publishing Group 2016-02-05 /pmc/articles/PMC4742815/ /pubmed/26847810 http://dx.doi.org/10.1038/srep20081 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Cai, Guanjing
Yang, Xujun
Lai, Qiliang
Yu, Xiaoqi
Zhang, Huajun
Li, Yi
Chen, Zhangran
Lei, Xueqian
Zheng, Wei
Xu, Hong
Zheng, Tianling
Lysing bloom-causing alga Phaeocystis globosa with microbial algicide: An efficient process that decreases the toxicity of algal exudates
title Lysing bloom-causing alga Phaeocystis globosa with microbial algicide: An efficient process that decreases the toxicity of algal exudates
title_full Lysing bloom-causing alga Phaeocystis globosa with microbial algicide: An efficient process that decreases the toxicity of algal exudates
title_fullStr Lysing bloom-causing alga Phaeocystis globosa with microbial algicide: An efficient process that decreases the toxicity of algal exudates
title_full_unstemmed Lysing bloom-causing alga Phaeocystis globosa with microbial algicide: An efficient process that decreases the toxicity of algal exudates
title_short Lysing bloom-causing alga Phaeocystis globosa with microbial algicide: An efficient process that decreases the toxicity of algal exudates
title_sort lysing bloom-causing alga phaeocystis globosa with microbial algicide: an efficient process that decreases the toxicity of algal exudates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742815/
https://www.ncbi.nlm.nih.gov/pubmed/26847810
http://dx.doi.org/10.1038/srep20081
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