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An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression

The Drosophila olfactory system is highly stereotyped in form and function; olfactory sensory neurons (OSNs) expressing a specific odorant receptor (OR) always appear in the same antennal location and the axons of OSNs expressing the same OR converge on the same antennal lobe glomeruli. Although som...

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Detalles Bibliográficos
Autores principales: Bhat, Shreelatha, Jones, Walton D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742857/
https://www.ncbi.nlm.nih.gov/pubmed/26848073
http://dx.doi.org/10.1038/srep20109
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author Bhat, Shreelatha
Jones, Walton D.
author_facet Bhat, Shreelatha
Jones, Walton D.
author_sort Bhat, Shreelatha
collection PubMed
description The Drosophila olfactory system is highly stereotyped in form and function; olfactory sensory neurons (OSNs) expressing a specific odorant receptor (OR) always appear in the same antennal location and the axons of OSNs expressing the same OR converge on the same antennal lobe glomeruli. Although some transcription factors have been implicated in a combinatorial code specifying OR expression and OSN identity, it is clear other players remain unidentified. In hopes of mitigating the challenges of genome-wide screening, we examined the feasibility of a two-tiered approach comprising a primary “pooling” screen for miRNAs whose tissue-specific over-expression causes a phenotype of interest followed by a focused secondary screen using gene-specific RNAi. Since miRNAs down-regulate their targets, miRNA over-expression phenotypes should be attributable to target loss-of-function. It is the sequence-dependence of miRNA-target pairing that suggests candidates for the secondary screen. Since miRNAs are short, however, miRNA misexpression will likely uncover non-biological miRNA-target relationships. Rather than focusing on miRNA function itself where these non-biological relationships could be misleading, we propose using miRNAs as tools to focus a more traditional RNAi-based screen. Here we describe such a screen that uncovers a role for Atf3 in the expression of the odorant receptor Or47b.
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spelling pubmed-47428572016-02-09 An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression Bhat, Shreelatha Jones, Walton D. Sci Rep Article The Drosophila olfactory system is highly stereotyped in form and function; olfactory sensory neurons (OSNs) expressing a specific odorant receptor (OR) always appear in the same antennal location and the axons of OSNs expressing the same OR converge on the same antennal lobe glomeruli. Although some transcription factors have been implicated in a combinatorial code specifying OR expression and OSN identity, it is clear other players remain unidentified. In hopes of mitigating the challenges of genome-wide screening, we examined the feasibility of a two-tiered approach comprising a primary “pooling” screen for miRNAs whose tissue-specific over-expression causes a phenotype of interest followed by a focused secondary screen using gene-specific RNAi. Since miRNAs down-regulate their targets, miRNA over-expression phenotypes should be attributable to target loss-of-function. It is the sequence-dependence of miRNA-target pairing that suggests candidates for the secondary screen. Since miRNAs are short, however, miRNA misexpression will likely uncover non-biological miRNA-target relationships. Rather than focusing on miRNA function itself where these non-biological relationships could be misleading, we propose using miRNAs as tools to focus a more traditional RNAi-based screen. Here we describe such a screen that uncovers a role for Atf3 in the expression of the odorant receptor Or47b. Nature Publishing Group 2016-02-05 /pmc/articles/PMC4742857/ /pubmed/26848073 http://dx.doi.org/10.1038/srep20109 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Bhat, Shreelatha
Jones, Walton D.
An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
title An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
title_full An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
title_fullStr An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
title_full_unstemmed An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
title_short An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression
title_sort accelerated mirna-based screen implicates atf-3 in drosophila odorant receptor expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742857/
https://www.ncbi.nlm.nih.gov/pubmed/26848073
http://dx.doi.org/10.1038/srep20109
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