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A genome-wide association study of fetal response to type 2 porcine reproductive and respiratory syndrome virus challenge

Control of porcine reproductive and respiratory syndrome (PRRS) is economically important for the swine industry worldwide. As current PRRS vaccines do not completely protect against heterologous challenge, alternative means of control, including enhanced genetic resilience, are needed. For reproduc...

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Autores principales: Yang, Tianfu, Wilkinson, James, Wang, Zhiquan, Ladinig, Andrea, Harding, John, Plastow, Graham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742883/
https://www.ncbi.nlm.nih.gov/pubmed/26846722
http://dx.doi.org/10.1038/srep20305
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author Yang, Tianfu
Wilkinson, James
Wang, Zhiquan
Ladinig, Andrea
Harding, John
Plastow, Graham
author_facet Yang, Tianfu
Wilkinson, James
Wang, Zhiquan
Ladinig, Andrea
Harding, John
Plastow, Graham
author_sort Yang, Tianfu
collection PubMed
description Control of porcine reproductive and respiratory syndrome (PRRS) is economically important for the swine industry worldwide. As current PRRS vaccines do not completely protect against heterologous challenge, alternative means of control, including enhanced genetic resilience, are needed. For reproductive PRRS, the genetic basis of fetal response to PRRS virus (PRRSV) infection is poorly understood. Genome-wide association studies (GWAS) were done here using data from 928 fetuses from pregnant gilts experimentally challenged with type 2 PRRSV. Fetuses were assessed for viral load in thymus (VLT), viral load in endometrium (VLE), fetal death (FD) and fetal viability (FV), and genotyped at a medium density. Collectively, 21 candidate genomic regions were found associated with these traits, seven of which overlap with previously reported QTLs for pig health and reproduction. A comparison with ongoing and related transcriptomic analyses of fetal response to PRRSV infection found differentially expressed genes within 18 candidate regions. Some of these genes have immune system functions, and have been reported to contribute to host response to PRRSV infection. The results provide new evidence about the genetic basis of fetal response to PRRSV challenge, and may ultimately lead to alternative control strategies to reduce the impact of reproductive PRRS.
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spelling pubmed-47428832016-02-09 A genome-wide association study of fetal response to type 2 porcine reproductive and respiratory syndrome virus challenge Yang, Tianfu Wilkinson, James Wang, Zhiquan Ladinig, Andrea Harding, John Plastow, Graham Sci Rep Article Control of porcine reproductive and respiratory syndrome (PRRS) is economically important for the swine industry worldwide. As current PRRS vaccines do not completely protect against heterologous challenge, alternative means of control, including enhanced genetic resilience, are needed. For reproductive PRRS, the genetic basis of fetal response to PRRS virus (PRRSV) infection is poorly understood. Genome-wide association studies (GWAS) were done here using data from 928 fetuses from pregnant gilts experimentally challenged with type 2 PRRSV. Fetuses were assessed for viral load in thymus (VLT), viral load in endometrium (VLE), fetal death (FD) and fetal viability (FV), and genotyped at a medium density. Collectively, 21 candidate genomic regions were found associated with these traits, seven of which overlap with previously reported QTLs for pig health and reproduction. A comparison with ongoing and related transcriptomic analyses of fetal response to PRRSV infection found differentially expressed genes within 18 candidate regions. Some of these genes have immune system functions, and have been reported to contribute to host response to PRRSV infection. The results provide new evidence about the genetic basis of fetal response to PRRSV challenge, and may ultimately lead to alternative control strategies to reduce the impact of reproductive PRRS. Nature Publishing Group 2016-02-05 /pmc/articles/PMC4742883/ /pubmed/26846722 http://dx.doi.org/10.1038/srep20305 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yang, Tianfu
Wilkinson, James
Wang, Zhiquan
Ladinig, Andrea
Harding, John
Plastow, Graham
A genome-wide association study of fetal response to type 2 porcine reproductive and respiratory syndrome virus challenge
title A genome-wide association study of fetal response to type 2 porcine reproductive and respiratory syndrome virus challenge
title_full A genome-wide association study of fetal response to type 2 porcine reproductive and respiratory syndrome virus challenge
title_fullStr A genome-wide association study of fetal response to type 2 porcine reproductive and respiratory syndrome virus challenge
title_full_unstemmed A genome-wide association study of fetal response to type 2 porcine reproductive and respiratory syndrome virus challenge
title_short A genome-wide association study of fetal response to type 2 porcine reproductive and respiratory syndrome virus challenge
title_sort genome-wide association study of fetal response to type 2 porcine reproductive and respiratory syndrome virus challenge
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742883/
https://www.ncbi.nlm.nih.gov/pubmed/26846722
http://dx.doi.org/10.1038/srep20305
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