NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force

In vertebrate cells, chromosomes oscillate to align precisely during metaphase. NuSAP, a microtubule-associated protein, plays a critical role in stabilizing spindle microtubules. In this study, we utilize 3D time-lapse live-cell imaging to monitor the role of NuSAP in chromosome oscillation and ide...

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Detalles Bibliográficos
Autores principales: Li, Chenyu, Xue, Chenyi, Yang, Qiaoyun, Low, Boon Chuan, Liou, Yih-Cherng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742958/
https://www.ncbi.nlm.nih.gov/pubmed/26839278
http://dx.doi.org/10.1038/ncomms10597
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author Li, Chenyu
Xue, Chenyi
Yang, Qiaoyun
Low, Boon Chuan
Liou, Yih-Cherng
author_facet Li, Chenyu
Xue, Chenyi
Yang, Qiaoyun
Low, Boon Chuan
Liou, Yih-Cherng
author_sort Li, Chenyu
collection PubMed
description In vertebrate cells, chromosomes oscillate to align precisely during metaphase. NuSAP, a microtubule-associated protein, plays a critical role in stabilizing spindle microtubules. In this study, we utilize 3D time-lapse live-cell imaging to monitor the role of NuSAP in chromosome oscillation and identify NuSAP as a novel regulator of the chromokinesin, Kid. Depletion of NuSAP significantly suppresses the amplitude and velocity of chromosome oscillation. We analyse the effects of NuSAP and Kid depletion in monopolar and bipolar cells with or without kinetochore microtubule depletion. Twelve postulated conditions are deciphered to reveal the contribution of NuSAP to the polar force generated at kinetochore microtubules and to the regulation of the polar ejection force generated by Kid, thus revealing a pivotal role of NuSAP in chromosome oscillation.
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spelling pubmed-47429582016-03-04 NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force Li, Chenyu Xue, Chenyi Yang, Qiaoyun Low, Boon Chuan Liou, Yih-Cherng Nat Commun Article In vertebrate cells, chromosomes oscillate to align precisely during metaphase. NuSAP, a microtubule-associated protein, plays a critical role in stabilizing spindle microtubules. In this study, we utilize 3D time-lapse live-cell imaging to monitor the role of NuSAP in chromosome oscillation and identify NuSAP as a novel regulator of the chromokinesin, Kid. Depletion of NuSAP significantly suppresses the amplitude and velocity of chromosome oscillation. We analyse the effects of NuSAP and Kid depletion in monopolar and bipolar cells with or without kinetochore microtubule depletion. Twelve postulated conditions are deciphered to reveal the contribution of NuSAP to the polar force generated at kinetochore microtubules and to the regulation of the polar ejection force generated by Kid, thus revealing a pivotal role of NuSAP in chromosome oscillation. Nature Publishing Group 2016-02-03 /pmc/articles/PMC4742958/ /pubmed/26839278 http://dx.doi.org/10.1038/ncomms10597 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Li, Chenyu
Xue, Chenyi
Yang, Qiaoyun
Low, Boon Chuan
Liou, Yih-Cherng
NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force
title NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force
title_full NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force
title_fullStr NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force
title_full_unstemmed NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force
title_short NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force
title_sort nusap governs chromosome oscillation by facilitating the kid-generated polar ejection force
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4742958/
https://www.ncbi.nlm.nih.gov/pubmed/26839278
http://dx.doi.org/10.1038/ncomms10597
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