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Comparative transcriptomics and comprehensive marker resource development in mulberry
BACKGROUND: High potential of Morus laevigata and Morus serrata has been proposed in the breeding programs for Morus sp. However, due to the lack of dense molecular markers this goal is still in its nascent stage and not yet realized. We thus, sequenced the transcriptomes of these two wild Morus spe...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743097/ https://www.ncbi.nlm.nih.gov/pubmed/26846165 http://dx.doi.org/10.1186/s12864-016-2417-8 |
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author | Saeed, Bushra Baranwal, Vinay K. Khurana, Paramjit |
author_facet | Saeed, Bushra Baranwal, Vinay K. Khurana, Paramjit |
author_sort | Saeed, Bushra |
collection | PubMed |
description | BACKGROUND: High potential of Morus laevigata and Morus serrata has been proposed in the breeding programs for Morus sp. However, due to the lack of dense molecular markers this goal is still in its nascent stage and not yet realized. We thus, sequenced the transcriptomes of these two wild Morus species and utilized the data for marker development. RESULTS: We generated 87.0 and 80.3 Mb of transcriptome data from M. laevigata and M. serrata, respectively. The transcriptomes from M. laevigata and M. serrata, were assembled into 95,181 and 85,269 transcripts, respectively, and annotated. We identified around 24,049 Simple Sequence Repeats (SSRs), 1,201,326 Single Nucleotide Polymorphisms (SNPs) and 67,875 Insertion-Deletions (InDels). The variants having a higher impact were also identified and their effect was further investigated. CONCLUSIONS: The transcriptome resource from the wildly growing mulberry species developed in this study can find wide applicability in gene identification and/or characterization. It can also contribute immensely in the existing mulberry improvement programs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2417-8) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4743097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47430972016-02-06 Comparative transcriptomics and comprehensive marker resource development in mulberry Saeed, Bushra Baranwal, Vinay K. Khurana, Paramjit BMC Genomics Research Article BACKGROUND: High potential of Morus laevigata and Morus serrata has been proposed in the breeding programs for Morus sp. However, due to the lack of dense molecular markers this goal is still in its nascent stage and not yet realized. We thus, sequenced the transcriptomes of these two wild Morus species and utilized the data for marker development. RESULTS: We generated 87.0 and 80.3 Mb of transcriptome data from M. laevigata and M. serrata, respectively. The transcriptomes from M. laevigata and M. serrata, were assembled into 95,181 and 85,269 transcripts, respectively, and annotated. We identified around 24,049 Simple Sequence Repeats (SSRs), 1,201,326 Single Nucleotide Polymorphisms (SNPs) and 67,875 Insertion-Deletions (InDels). The variants having a higher impact were also identified and their effect was further investigated. CONCLUSIONS: The transcriptome resource from the wildly growing mulberry species developed in this study can find wide applicability in gene identification and/or characterization. It can also contribute immensely in the existing mulberry improvement programs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2417-8) contains supplementary material, which is available to authorized users. BioMed Central 2016-02-04 /pmc/articles/PMC4743097/ /pubmed/26846165 http://dx.doi.org/10.1186/s12864-016-2417-8 Text en © Saeed et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Saeed, Bushra Baranwal, Vinay K. Khurana, Paramjit Comparative transcriptomics and comprehensive marker resource development in mulberry |
title | Comparative transcriptomics and comprehensive marker resource development in mulberry |
title_full | Comparative transcriptomics and comprehensive marker resource development in mulberry |
title_fullStr | Comparative transcriptomics and comprehensive marker resource development in mulberry |
title_full_unstemmed | Comparative transcriptomics and comprehensive marker resource development in mulberry |
title_short | Comparative transcriptomics and comprehensive marker resource development in mulberry |
title_sort | comparative transcriptomics and comprehensive marker resource development in mulberry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743097/ https://www.ncbi.nlm.nih.gov/pubmed/26846165 http://dx.doi.org/10.1186/s12864-016-2417-8 |
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